Assessing and predicting the changes for inorganic mercury and methylmercury concentrations in surface waters of a tidal estuary (Adour Estuary, SW France).

Total and dissolved concentrations of inorganic mercury (IHg) and methylmercury (MeHg) in water (Adour Estuary) were determined during three sampling campaigns and related to biogeochemical variables (nutrients, organic matter). Factors (sampling time, sample type) were included in analysis of covariance with effect separation. The urban estuary suffered historically from anthropogenic sources, however, decreased emissions have reduced Hg concentrations. Total IHg (0.51-3.42 ng L-1) and MeHg (25-81 pg L-1) concentrations are additively described by suspended particulate matter and particulate organic carbon. Higher total concentrations, carried by organic-rich particles, were found near specific discharge points (0.79-8.02 ng L-1 and 34-235 pg L-1 for IHg and MeHg, respectively). The associated high dissolved MeHg concentrations could not be explained only by biogeochemical variables. Better efficiency of the models is found for total than for dissolved concentrations. Models should be checked with other contaminants or with estuaries, suffering from downstream contamination.

Keystone microbial taxa organize micropollutant-related modules shaping the microbial community structure in estuarine sediments.

The fluctuation of environmental conditions drives the structure of microbial communities in estuaries, highly dynamic ecosystems. Microorganisms inhabiting estuarine sediments play a key role in ecosystem functioning. They are well adapted to the changing conditions, also threatened by the presence of pollutants. In order to determine the environmental characteristics driving the organization of the microbial assemblages, we conducted a seasonal survey along the Adour Estuary (Bay of Biscay, France) using 16S rRNA gene Illumina sequencing. Microbial diversity data were combined with a set of chemical analyses targeting metals and pharmaceuticals. Microbial communities were largely dominated by Proteobacteria (41 %) and Bacteroidota (32 %), showing a strong organization according to season, with an important shift in winter. The composition of microbial communities showed spatial distribution according to three main areas (upstream, middle, and downstream estuary) revealing the influence of the Adour River. Further analyses indicated that the microbial community was influenced by biogeochemical parameters (Corg/Norg and δ13C) and micropollutants, including metals (As, Cu, Mn, Sn, Ti, and Zn) and pharmaceuticals (norfloxacin, oxolinic acid and trimethoprim). Network analysis revealed specific modules, organized around keystone taxa, linked to a pollutant type, providing information of paramount importance to understand the microbial ecology in estuarine ecosystems.