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Transfusion ; 55(5): 1028-32, 2015 May.
Artigo em Inglês | MEDLINE | ID: mdl-25472857

RESUMO

BACKGROUND: Processed umbilical cord blood (UCB) must be stored at cryogenic temperature at all times to maintain the quality and viability of the cells. However, a challenge is presented in the form of moving a large number of cryopreserved UCB samples to a new location. In this report, we share our experience on relocating more than 100,000 units of cryopreserved UCB samples stored in 12 liquid nitrogen freezers (LNFs) to our new laboratory. STUDY DESIGN AND METHODS: For quality control purposes, 2 weeks before relocation, donor UCB samples were processed, cryopreserved, and stored in each LNF. On relocation day, half of the samples were retrieved to determine total nucleated cell count, percentage of CD34+ cells, and cell viability as controls for later comparison. UCB samples were transferred into dry shippers before being relocated to the new laboratory. Upon arrival, LNFs were serviced before transferring UCB samples back into its original location within the LNF. The remaining donor UCB samples were retrieved and analyzed for the same tests mentioned. RESULTS: We found no significant differences in pre- and postrelocation values of the tests performed. CONCLUSION: All UCB samples were successfully relocated into the new laboratory without affecting the quality.


Assuntos
Criopreservação/métodos , Sangue Fetal , Antígenos CD34/metabolismo , Humanos , Temperatura
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