Treatment and recovery of phosphate from submerged anaerobic membrane bioreactor effluent using thermally treated biowaste and powder activated carbon.

This study presents a novel treatment system using a submerged anaerobic membrane bioreactor (SAnMBR) followed by adsorption onto thermally treated biowaste, and ending with a final treatment using powdered activated carbon (PAC). Despite limited phosphate and ammonium ion removal during SAnMBR operation, thermally treated eggshell (EGSL) and seagrass (SG) received SAnMBR effluent and enhanced phosphate recovery, achieving removal rates of 71.8-99.9% and 60.5-78.0%, respectively. The SAnMBR achieved an 85% COD removal, which was slightly reduced further by biowaste treatment. However, significant further reductions in COD to 20.2 ± 5.2 mg/L for EGSL effluent and 57.0 ± 13.3 mg/L for SG effluent were achieved with PAC. Phytotoxicity tests showed the SAnMBR effluent after PAC treatment notably improved seed growth compared to untreated wastewater. In addition, volatile organic compounds (VOCs) were significantly reduced in the system, including common wastewater contaminants such as dimethyl disulfide, dimethyl trisulfide, phenol, p-cresol, nonanal, and decanal. Fractionation analysis of the solid fraction, post-adsorption from both synthetic and domestic wastewater, indicated that for SG, 77.3%-94% of the total phosphorus (TP) was inorganically bound, while for EGSL, it ranged from 94% to 95.3%. This study represents the first attempt at a proof-of-concept for simultaneous treatment of domestic wastewater and phosphorus recovery using this integrated system.

Combined use of strictly anaerobic MBBR and aerobic MBR for municipal wastewater treatment and removal of pharmaceuticals.

An integrated lab-scale wastewater treatment system consisting of an anaerobic Moving Bed Biofilm Reactor (AnMBBR) and an aerobic Membrane Bioreactor (AeMBR) in series was used to study the removal and fate of pharmaceuticals during wastewater treatment. Continuous-flow experiments were conducted applying different temperatures to the AnMBBR (Phase A: 35 °C; Phase B: 20 °C), while batch experiments were performed for calculating sorption and biotransformation kinetics. The total removal of major pollutants and target pharmaceuticals was not affected by the temperature of the AnMBBR. In Phase A, the average removal of dissolved chemical oxygen demand (COD), biological oxygen demand (BOD), and ammonium nitrogen (NH4-N) was 86%, 91% and 96% while in Phase B, 91%, 96% and 96%, respectively. Removal efficiencies ranging between 65% and 100% were observed for metronidazole (MTZ), trimethoprim (TMP), sulfamethoxazole (SMX), and valsartan (VAL), while slight (<30%) or no removal was observed for carbamazepine (CBZ) and diclofenac (DCF), respectively. Application of a mass balance model showed that the predominant mechanism for the removal of pharmaceuticals was biotransformation, while the role of sorption was of minor importance. The AeMBR was critical for VAL, SMX and TMP biodegradation; the elimination of MTZ was strongly enhanced by the AnMBBR. In both bioreactors, Bacteroidetes was the dominant phylum in both bioreactors over time. In the AnMBBR, Cloacibacterium and Bacteroides had a higher abundance in the biocarriers compared to the suspended biomass.

Dairy wastewater management in EU: Produced amounts, existing legislation, applied treatment processes and future challenges.

Dairy industry consumes high water amounts and generates highly contaminated wastewater. EU-27 is the second largest milk producer and the main cheese exporter in the world. The main objectives of the current study was to estimate the amounts of dairy wastewater (DWW) that are produced annually in different EU countries and to present the relevant existing EU legislation. The main treatment practices currently applied as well as the future opportunities for sustainable DWW management were also discussed. According to the results a total amount of 192.5 × 106 m3 of DWW are annually produced in EU-27 countries, 49% of them are due to the production of cheeses, while 19%, 18% and 13% are due to the production of drinking milk, acidified milk and butterfat products, respectively. Six countries (Germany, France, Italy, Poland, Spain and Netherlands) contribute to the generation of more than 73% of DWW, while the annual per capita DWW production ranges between 36 L (Luxembourg) and 1441 L (Ireland). Since 2019, EU has established best available techniques (BAT) for the dairy industry in order to achieve efficient monitoring of the produced wastewater, reduced water consumption and increased resource efficiency. The main on-site treatment processes that are currently applied include in series wastewater pretreatment for the removal of fat and pH adjustment, anaerobic or/and aerobic biological processes for the decrease of organic loading and nutrients and use of membranes for the cases that recovered water is going to be reused. Limited information is so far available for the operational treatment cost of the different processes. Data originated from a large dairy industry in Cyprus showed an operational cost equal to 1.21 €/m3 of treated wastewater. The main future challenge for the dairy industry and water treatment sector is the adoption of novel processes aiming to DWW valorization under the frame of circular economy.

In situ biogas upgrading and enhancement of anaerobic digestion of cheese whey by addition of scrap or powder zero-valent iron (ZVI).

Cheese whey is an easily biodegradable substrate with high organic matter that can be anaerobically digested to biogas; however, the process is often inhibited by excess acidification due to the presence of undissociated volatile fatty acids and requires considerable concentration of alkaline buffer. The current study investigates a new approach for biogas upgrading, and increase of total CH4 in conjunction with buffering acidification by using zero-valent iron (powder and scrap metals at concentrations 25, 50, and 100 g/L) in anaerobic granular sludge and cheese whey under mesophilic batch conditions. During the first 2 cycles (total 34 days), a high performance was found in anaerobic bottles with 25 g/L powder zero valent iron (PZVI) and 50 g/L scrap zero valent iron (SZVI) since they had a higher total CH4 production compared to anaerobic bottles free of ZVI, as well as 97% CH4 composition in produced biogas compared to 74% CH4 for anaerobic bottles free of ZVI. Under these conditions, no additional NaOH was added to anaerobic bottles with 25 g/L PZVI and 50 g/L SZVI to increase the pH and at the end of 2nd cycle the concentration of VFAs was substantially lower compared to the anaerobic bottles free of ZVI. However, no positive effects of ZVI in terms of alkaline buffer were found at the 3rd and 4th cycle probably due to ZVI inactivation outer surface layer. Based on the experimental findings (anaerobic bottles: (a) 25 g/L PZVI, (b) 50 g/L SZVI and (c) free of ZVI) an economic comparison for anaerobic digestion of cheese whey by large scale was contacted and pointed out that the best scenario was the anaerobic digestion by addition of 50 g/L SZVI, followed by anaerobic digestion free of ZVI and last was the anaerobic digestion by addition of 25 g/L PZVI. This study highlights a new proof of concept for in-situ biogas upgrading and alleviation of acidification by addition of 50 g/L SZVI or 25 g/L PZVI during anaerobic digestion of cheese whey.

Recovery of phosphate from dewatered anaerobic sludge and wastewater by thermally treated P.oceanica residues and its potential application as a fertilizer.

A process for the valorization of seagrass residues has been developed, aiming to investigate its potential as a phosphate adsorbent and the production of added-value products, which can be assessed in agricultural applications. Posidonia oceanica residues were thermally treated (500 °C, 1 h) and were tested as an adsorbent of phosphate from real wastewater. Chemical leaching experiments of phosphate from dewatered anaerobic sludge (DWAS) were conducted, evaluating sonication and inorganic acids (sulfuric acid (SA), thermal-sulfuric acid (TSA), and nitric acid (NA)) as extraction methods. Τhe extraction efficiency of each method tested demonstrated that the most suitable acid medium to leach out phosphate were SA and TSA processes with 84.9 and 93.2% removal efficiency, respectively. Moreover, the saturation capacity of thermally treated P. oceanica was assessed, and the results confirmed the high phosphate adsorption capacity (179.1 mg g-1). Adsorption batch experiments with real wastes (anaerobic effluent wastewater and leached solution from DWAS), demonstrated that thermally treated seagrass could have a high adsorption efficiency and selectivity towards phosphate. After phosphate adsorption, the solid residues were mixed with compost in different ratio and then tested as fertilizer substitutes on plant growth. The solid residue after adsorption produced from anaerobic effluent or synthetic solutions imposed a positive effect on plant growth with germination index (GI) values 96.7-111.14%, for all types of seeds tested (Solanum Lycopersicum, Lepidium sativum, and Sinapis alba), while the solid residue after adsorption produced from DWAS leached solution negatively affected the germination of plants, probably due to potentially refractory compounds contained in DWAS.

A Citrus Peel Waste Biorefinery for Ethanol and Methane Production.

This paper deals with the development of a citrus peel waste (CPW) biorefinery that employs low environmental impact technologies for production of ethanol and methane. Three major yeasts were compared for ethanol production in batch fermentations using CPW pretreated through acid hydrolysis and a combination of acid and enzyme hydrolysis. The most efficient conditions for production of CPW-based hydrolyzates included processing at 116 °C for 10 min. Pichia kudriavzevii KVMP10 achieved the highest ethanol production that reached 30.7 g L-1 in fermentations conducted at elevated temperatures (42 °C). A zero-waste biorefinery was introduced by using solid biorefinery residues in repeated batch anaerobic digestion fermentations achieving methane formation of 342 mL gVS-1 (volatile solids). Methane production applying untreated and dried CPW reached a similar level (339-356 mL gVS-1) to the use of the side stream, demonstrating that the developed bioprocess constitutes an advanced alternative to energy intensive methods for biofuel production.

Biodegradation of bilge water: Batch test under anaerobic and aerobic conditions and performance of three pilot aerobic Moving Bed Biofilm Reactors (MBBRs) at different filling fractions.

The bilge water that is stored at the bottom of the ships is saline and greasy wastewater with a high Chemical Oxygen Demand (COD) fluctuations (2-12 g COD L-1). The aim of this study was to examine at a laboratory scale the biodegradation of bilge water using first anaerobic granular sludge followed by aerobic microbial consortium (consisted of 5 strains) and vice versa and then based on this to implement a pilot scale study. Batch results showed that granular sludge and aerobic consortium can remove up to 28% of COD in 13 days and 65% of COD removal in 4 days, respectively. The post treatment of anaerobic and aerobic effluent with aerobic consortium and granular sludge resulted in further 35% and 5% COD removal, respectively. The addition of glycine betaine or nitrates to the aerobic consortium did not enhance significantly its ability to remove COD from bilge water. The aerobic microbial consortium was inoculated in 3 pilot (200 L) Moving Bed Biofilm Reactors (MBBRs) under filling fractions of 10%, 20% and 40% and treated real bilge water for 165 days under 36 h HRT. The MBBR with a filling fraction of 40% resulted in the highest COD decrease (60%) compared to the operation of the MBBRs with a filling fraction of 10% and 20%. GC-MS analysis on 165 day pointed out the main organic compounds presence in the influent and in the MBBR (10% filling fraction) effluent.

Compatible solute addition to biological systems treating waste/wastewater to counteract osmotic and other environmental stresses: a review.

This study reviews the addition of compatible solutes to biological systems as a strategy to counteract osmolarity and other environmental stresses. At high osmolarity many microorganisms accumulate organic solutes called "compatible solutes" in order to balance osmotic pressure between the cytoplasm and the environment. These organic compounds are called compatible solutes because they can function inside the cell without the need for special adaptation of the intracellular enzymes, and also serve as protein stabilizers in the presence of high ionic strength. Moreover, the compatible solutes strategy is regularly being employed by the cell, not only under osmotic stress at high salinity, but also under other extreme environmental conditions such as low temperature, freezing, heat, starvation, dryness, recalcitrant compounds and solvent stresses. The accumulation of these solutes from the environment has energetically a lower cost than de novo synthesis. Based on this cell mechanism several studies in the field of environmental biotechnology (most of them on biological wastewater treatment) employed this strategy by exogenously adding compatible solutes to the wastewater or medium in order to alleviate environmental stress. This current paper critically reviews and evaluates these studies, and examines the future potential of this approach. In addition to this, a strategy for the successful implementation of compatible solutes in biological systems is proposed.

Novel Halomonas sp. B15 isolated from Larnaca Salt Lake in Cyprus that generates vanillin and vanillic acid from ferulic acid.

Vanillin is a high value added product with many applications in the food, fragrance and pharmaceutical industries. A natural and low-cost method to produce vanillin is by microbial bioconversions through ferulic acid. Until now, limited microorganisms have been found capable of bioconverting ferulic acid to vanillin at high yield. This study aimed to screen halotolerant strains of bacteria from Larnaca Salt Lake which generate vanillin and vanillic acid from ferulic acid. From a total of 50 halotolenant/halophilic strains 8 grew in 1 g/L ferulic acid and only 1 Halomonas sp. B15 and 3 Halomonas elognata strains were capable of bioconverting ferulic acid to vanillic acid at 100 g NaCl/L. The highest vanillic acid (365 mg/L) at these conditions generated by Halomonas sp. B15 which corresponds to ferulic acid bioconversion yield of 36.5%. Using the resting cell technique with an initial ferulic acid concentration of 0.5 g/L at low salinity, the highest production of vanillin (245 mg/L) took place after 48 h, corresponding to a bioconversion yield of 49%. This is the first reported Halomonas sp. with high yield of vanillin production from ferulic acid at low salinity.

Effect of bicarbonate on hydrogen generation by Zero-Valent iron and its impact on generation of acetic acid by seven different inocula.

This study demonstrates the substantial role of bicarbonate within a zero-valent iron (ZVI) system in hydrogen evolution, demonstrating that heightened concentration levels notably enhance hydrogen output. The acetic acid performance production of seven different inocula was examined when exposed to ZVI and CO2 as the sole carbon source, separately. Along the seven inocula, river and constructed wetland sludges show the highest production rates at 300 mg/L day-1 and 269 mg/L day-1, respectively. Acetobacterium levels significantly rose in CO2-enriched environments, particularly in river and wetland sludges. Moreover, bacteria attached to ZVI showed accelerated hydrogen consumption and acetic acid production compared to their freely suspended or ZVI-detached counterparts when hydrogen was primarily added externally. This study highlighted the positive effect of high concentrations of soluble CO2, which acted both as a reactant with ZVI for hydrogen production and as a substrate for homoacetogens, leading to high acetic acid generation.

Study on the fate of per- and polyfluoroalkyl substances during thermophilic anaerobic digestion of sewage sludge and the role of granular activated carbon addition.

Limited information is available on the removal of per- and polyfluoroalkyl substances (PFAS) in anaerobic digestion (AD). Τhe fate of six PFAS was studied in thermophilic bioreactors in the presence of granular activated carbon (GAC) and voltage application. Reactors with GAC exhibited lower concentrations of volatile fatty acids and higher methane production compared to those with and without the application of voltage. Analysis of PFAS in dissolved and solid phase showed that their distribution was dependent on perfluorocarbon chain length and functional group. Mass balances showed that PFAS were not removed during conventional AD or after applying voltage; however, significant removal (up to 61 ± 8 %) was observed in bioreactors with GAC for perfluoroheptanoic acid (PFHpA), perfluorooctanoic acid (PFOA), perfluorononanoic acid (PFNA), and perfluorooctane sulfonate (PFOS). Biomass characterization showed that in these bioreactors, the relative abundance of Acinetobacter and Pseudomonas was higher, indicating their potential role in PFAS biotransformation.

Treatment of high-strength saline bilge wastewater by four pilot-scale aerobic moving bed biofilm reactors and comparison of the microbial communities.

Four Pilot-scale Moving Bed Biofilm Reactors (MBBRs) were operated for the treatment of real, saline, bilge wastewater. The MBBRs were connected in pairs to create two system configurations with different filling ratios (20%, 40%) and were operated in parallel. The inflow organic loading rate (OLR) varied from 3.6 ± 0.2 to 7.8 ± 0.6 g COD L-1 d-1, salinity was >15 ppt and three hydraulic residence times (HRTs) were tested 48, 30 and 24 h. In both systems, the first-stage bioreactors (R1 and R3) eliminated the higher part of the organic load (57%-65%). The second-stage bioreactors (R2 and R4) removed an additional fraction (18%-31%) of the organic load received by the effluent of R1 and R3, respectively. The microbial communities of the influent wastewater, suspended, and attached biomass were determined using 16S rRNA gene amplicon sequencing analysis. The evolution of the microbial communities was investigated and compared over the different operational phases. The microbial communities of the biofilm presented higher diversity and greater stability in composition over time, while the suspended biomass exhibited intense and rapid changes in the dominance of genera. Proteobacteria, Bacteroidetes and Firmicutes were highly present in the biofilm. The genera Celeribacter, Novispirillum, Roseovarius (class: Alphaproteobacteria) and Formosa (class: Flavobacteriia) were highly present during all operational phases. Principal Component Analysis (PCA) was used to identify similarities between samples, exhibiting high relation of samples according to the series of the bioreactor (1st, 2nd).

Microbial electrolysis cell coupled with anaerobic granular sludge: A novel technology for real bilge water treatment.

In the current study, treatment of undiluted real bilge water (BW) and the production of methane was examined for the first time using a membraneless single chamber Microbial Electrolysis Cell (MEC) with Anaerobic Granular Sludge (AGS) for its biodegradation. Initially, Anaerobic Toxicity Assays (ATAs) were used to evaluate the effect of undiluted real BW on the methanogenic activity of AGS. According to the results, BW shown higher impact to acetoclastics compared to hydrogenotrophic methanogens which proved to be more tolerant. However, dilution of BW caused lower inhibition allowing BW biodegradation. Maximum methane production (142.2 ± 4.8 mL) was observed at 50% of BW. Operation of MEC coupled with AGS, seemed to be very promising technology for BW treatment. During 80 days of operation in increasing levels of BW, R2 (1 V) reactor resulted in better performance than AGS alone. Exposure of AGS to gradual increase of BW content revealed that CH4 production was possible and reached 51% in five days even after feeding with 90% of BW using simple commercial iron electrodes. Successful chemical oxygen demand (sCOD) removal (up to 70%) was observed after gradual biomass acclimatization. Among the different monitored volatile fatty acids (VFAs), acetic and valeric acids were the most frequently detected compounds with concentrations up to 2.79 and 1.81 g L-1, respectively. The recalcitrant nature of BW did not allow the MEC-AD (anaerobic digester) to balance the consumed energy. Microbial profile analysis confirmed the existence of several methanogenic microorganisms of which Desulfovibrio and Methanobacterium presented significantly higher abundance in the cathodes compared to anodes and AGS.

Microbial Electrosynthesis Inoculated with Anaerobic Granular Sludge and Carbon Cloth Electrodes Functionalized with Copper Nanoparticles for Conversion of CO2 to CH4.

Microbial electrosynthesis (MES) can sustainably convert CO2 to products and significant research is currently being conducted towards this end, mainly in laboratory-scale studies. The high-cost ion exchange membrane, however, is one of the main reasons hindering the industrialization of MES. This study investigates the conversion of CO2 (as a sole external carbon source) to CH4 using membraneless MES inoculated with anaerobic granular sludge. Three types of electrodes were tested: carbon cloth (CC) and CC functionalized with Cu NPs, where Cu NPs were deposited for 15 and 45 min, respectively. During the MES experiment, which lasted for 144 days (six cycles), methane was consistently higher in the serum bottles with CC electrodes and applied voltage. The highest CH4 (around 46%) was found in the second cycle after 16 days. The system's performance declined during the following cycles; nevertheless, the CH4 composition was twice as high compared to the serum bottles without voltage. The MES with Cu NPs functionalized CC electrodes had a higher performance than the MES with plain CC electrodes. Microbial profile analysis showed that the Methanobacterium was the most dominant genus in all samples and it was found in higher abundance on the cathodes, followed by the anodes, and then in the suspended biomass. The genus Geobacter was identified only on the anodes regarding relative bacterial abundance at around 6-10%. Desulfovibrio was the most dominant genus in the cathodes; however, its relative abundance was significantly higher for the cathodes with Cu NPs.

Biogas upgrading to methane and removal of volatile organic compounds in a system of zero-valent iron and anaerobic granular sludge.

The current study presented a novel process of biogas upgrading to biomethane (higher than 97%) based on anaerobic sludge and zero-valent iron (ZVI) system. When ZVI was added into an aquatic system with anaerobic granular sludge (AnGrSl) under anaerobic abiotic conditions, H2 was generated. Then, the H2 and CO2 were converted by the hydrogenotrophic methanogens to CH4. Biogas upgrading to biomethane was achieved in 4 days in the AnGrSl system (50 g L-1 ZVI, initial pH 5 and 20 g L-1 NaHCO3). In this system, when zero-valent scrap iron (ZVSI) was added instead of ZVI, a more extended period (21 days) was required to achieve biogas upgrading. X-ray diffraction (XRD) analysis revealed that the materials in a reactor with CO2 or biogas headspace, exhibited a mixture of ferrite and the iron carbonate phase of siderite (FeCO3), with the latter being the dominant phase. VOCs analysis in raw biogas (in the system of anaerobic sludge and ZVI) highlighted the reduction of low mass straight- and branched-chain alkanes (C6-C10). Also, H2S and NH3 were found to be substantially reduced when the anaerobic sludge was exposed to ZVI compared to the cases where ZVI was not added. This study found that simultaneously with biogas upgrading, VOCs, H2S and NH3 can be removed in a system of ZVI or ZVSI and AnGrSl under aquatic anaerobic conditions.

Oil biodesulfurization: A review of applied analytical techniques.

The wide use of fossil fuels and their associated environmental concerns, highlighted the importance of affordable and clean energy (goal 7), as adopted by the Sustainable Development Goals of the United Nations for 2030. For years now, the detection of sulfur components in liquid fuels is performed mainly for environmental and health purposes in compliance with the respective legislations. Towards this, the aerobic and anaerobic biodesulfurization (BDS) process, which entails the use of microorganisms to limit the sulfur concentration is followed. To ensure effective BDS, several traditional analytical methods are utilized, although they require bench-top, bulky, costly, and time-consuming instruments along with skilled personnel. The currently employed analytical methods are mostly chromatographic techniques (e.g. liquid and gas) coupled with various detectors. To start with, high-performance liquid chromatography with ultraviolet detector (HPLC-UV), as well as electrospray ionization-LC-mass spectrometry (ESI-LC-MS) were mostly reported. Additionally, many detectors were coupled to gas chromatography (CG) including atomic emission detector (GC-AED), flame ionization detector (GC-FID), flame photometric detector (GC-FPD), sulfur fluorescence detector (GC-SFD), mass selective detector (GC-MS), etc. The solid-phase microextraction (SPME) technique provides extra capabilities when added to the separation techniques. Towards the continuous interest in oil supercomplex synthesis, other atmospheric and surface desorption ionization techniques, as well as the multidimensional 2D chromatographic systems (GC × GC and LC × LC) were also investigated, due to their unsurpassed resolution power. The current review ends with final remarks per applied methodology and the necessity to respect and protect the human environment and life.

Comparison of different cultures and culturing conditions for the biological deterioration of organic load from real saline bilge wastewater: microbial diversity insights and ecotoxicity assessment.

Bilge wastewater is a high strength, typically saline wastewater, originating from operation of ships. In this study, the treatment of real bilge wastewater was tested using pure isolated aerobic strains and mixed cultures (aerobic and anaerobic). The Chemical Oxygen Demand (COD) and ecotoxicity decrease were monitored over time, while the microbial dynamics alterations in mixed cultures were also recorded. The isolated strains Pseudodonghicola xiamenensis, Halomonas alkaliphila and Vibrio antiquaries were shown to significantly biodegrade bilge wastewater. Reasonable COD removal rates were achieved by aerobic mixed cultures (59%, 9 days), while anaerobic mixed cultures showed lower performance (34%, 51 days). The genus Pseudodonghicola was identified as dominant under aerobic conditions both in the mixed cultures and in the control sample (raw wastewater), after exposure to bilge wastewater, demonstrating natural proliferation of the genus and potential contribution to COD reduction. Biodegradation rates were higher when initial organic load was high, while the toxicity of raw wastewater partially decreased after treatment.

Enhanced biodegradation and valorization of drilling wastewater via simultaneous production of biosurfactants and polyhydroxyalkanoates by Pseudomonas citronellolis SJTE-3.

Pseudomonas citronellolis SJTE-3 was isolated as a highly efficient microorganism for biodegradation and valorization of drilling fluids (DF) wastewater. The strain metabolised DF and oily mud exhibiting up to 93%, 86%, 85% and 88% of chemical oxygen demand (COD), n-dodecane, n-tetradecane and naphthalene removal efficiency respectively. Enhanced bioconversion was enabled through production of biosurfactants that reduced the surface tension of water by 53% and resulted in 43.3% emulsification index (E24), while synthesizing 24% of dry cell weight (DCW) as medium-chain-length polyhydroxyalkanoates (PHA). Expression from the main pathways for alkanes and naphthalene biodegradation as well as biosurfactants and PHA biosynthesis revealed that although the alkanes and naphthalene biodegradation routes were actively expressed even at stationary phase, PHA production was stimulated at late stationary phase and putisolvin could comprise the biosurfactant synthesized. The bioconversion of toxic petrochemical residues to added-value thermoelastomers and biosurfactants indicate the high industrial significance of P. citronellolis SJTE-3.

Improving Biological Treatment of Real Bilge Wastewater With Zero Valent Iron and Activated Charcoal Addition.

From the ships engine rooms a recalcitrant wastewater is produced called "bilge" which contains oil, metal working fluids, surfactants, and salinity. This study investigated the treatment of real bilge wastewater in short experiments using the following processes: (i) anaerobic digestion with granular sludge and ZVI addition for enhancement of methane production, (ii) activated charcoal addition to biological treatment (aerobic and anaerobic) for Chemical Oxygen Demand (COD) significant reduction and (iii) combination of ZVI and anaerobic charcoal addition for high performance treatment. The addition of ZVI in anaerobic sludge resulted in higher performance mostly in cumulative CH4 production. The microbial profile of anaerobic granular sludge exposed to ZVI was determined and Acetobacterium and Arcobacter were the most dominant bacteria genera. Activated charcoal achieved higher COD removal, compared to biological degradation (aerobic and anaerobic). The combination of the two mechanisms, activated charcoal and biomass, had higher COD removal only for aerobic biomass. The combination of ZVI and activated charcoal to anaerobic digestion resulted in higher CH4 production and significant COD removal in short contact time.

Selection and assembly of indigenous bacteria and methanogens from spent metalworking fluids and their potential as a starting culture in a fluidized bed reactor.

Waste metalworking fluids (MWFs) are highly biocidal resulting in real difficulties in the, otherwise favoured, bioremediation of these high chemical oxygen deman (COD) wastes anaerobically in bioreactors. We have shown, as a proof of concept, that it is possible to establish an anaerobic starter culture using strains isolated from spent MWFs which are capable of reducing COD or, most significantly, methanogenesis in this biocidal waste stream. Bacterial strains (n = 99) and archaeal methanogens (n = 28) were isolated from spent MWFs. The most common bacterial strains were Clostridium species (n = 45). All methanogens were identified as Methanosarcina mazei. Using a random partitions design (RPD) mesocosm experiment, we found that bacterial diversity and species-species interactions had significant effects on COD reduction but that bacterial composition did not. The RPD study showed similar effects on methanogenesis, except that composition was also significant. We identified bacterial species with positive and negative effects on methane production. A consortium of 16 bacterial species and three methanogens was used to initiate a fluidized bed bioreactor (FBR), in batch mode. COD reduction and methane production were variable, and the reactor was oscillated between continuous and batch feeds. In both microcosm and FBR experiments, periodic inconsistencies in bacterial reduction in fermentative products to formic and acetic acids were identified as a key issue.