Fluorinated bamboo-structure carbon nanotubes: as attractive substrates for the cathodes of lithium-sulfur batteries.

Lithium-sulfur (Li-S) batteries have gained considerable attention for high theoretical specific capacity and energy density. However, their development is hampered by the poor electrical conductivity of sulfur and the shuttle of polysulfides. Herein, the acidified bamboo-structure carbon nanotubes (BCNTs) were mixed with polyvinylidene difluoride and pyrolyzed at high-temperature to obtain the fluorinated bamboo-structure carbon nanotubes (FBCNTs), which were compounded with sulfur as the cathode. The prepared S@FBCNTs with sulfur loading reaching 74.2 wt.% shows a high initial specific capacity of 1407.5 mAh·g-1at the discharge rate of 0.1 C. When the discharge rate was increased to 5 C, the capacity could be maintained at 622.3 mAh·g-1. The electrical conductivity of carbon nanotubes is effectively improved by semi-ionic C-F bonds formed by the doped F atoms and carbon atoms. Simultaneously, the surface of the F-containing carbon tubes exhibits strong polarity and strong chemisorption effect on polysulfides, which inhibits the shuttle effect of Li-S batteries.

Preparation and Performance of Lignin-Based Multifunctional Superhydrophobic Coating.

Superhydrophobic coatings have drawn much attention in recent years for their widespread potential applications. However, there are challenges to find a simple and cost-effective approach to prepare superhydrophobic materials and coatings using natural polymer. Herein, we prepared a kraft lignin-based superhydrophobic powder via modifying kraft lignin through 1H, 1H, 2H, 2H-perfluorodecyl-triethoxysilane (PFDTES) substitution reaction, and constructed superhydrophobic coatings by direct spraying the suspended PFDTES-Lignin powder on different substrates, including glass, wood, metal and paper. The prepared lignin-based coatings have excellent repellency to water, with a water contact angle of 164.7°, as well as good friction resistance, acid resistance, alkali resistance, salt resistance properties and quite good self-cleaning performance. After 30 cycles of sand friction or being stayed in 2 mol/L HCl, 0.25 mol/L NaOH and 2 mol/L NaCl solution for 30 min, the coatings still retain super hydrophobic capability, with contact angles higher than 150°. The superhydrophobic performance of PFDTES-Lignin coatings is mainly attributed to the constructed high surface roughness and the low surface energy afforded by modified lignin. This lignin-based polymer coating is low-cost, scalable, and has huge potential application in different fields, providing a simple way for the value-added utilization of kraft lignin.

Calixarene-Protected Titanium-Oxo Clusters and Their Photocurrent Responses and Photocatalytic Performances.

Three photosensitive tert-butylcalix[n]arene (TBC[n], n = 4, 6, 8)-protected titanium-oxo clusters (TOCs), formulated as [Ti4(µ3-O)2(TBC[4])2(OiPr)4(DEF)2]·DEF (1, TBC[4]-Ti4, DEF = N,N-diethylformamide), [Ti4(µ4-O)TBC[6](OCH3)9]·H2O (2, TBC[6]-Ti4), and [Ti4(µ3-O)2(OiPr)4TBC[8](DEF)2]·DEF (3, TBC[8]-Ti4), were successfully synthesized and characterized. Because of the generation of charge transfer from TBC[n] to the TiO core, the three TBC[n]-decorated TOCs show a broadened visible-light absorption and narrowed optical band gap based on the UV-visible spectra and density functional theory calculations. The corresponding photosensitive electrodes prepared using these three TOCs exhibit stable photocurrent responses. Furthermore, their photocatalytic performances for hydrogen evolution and methylene blue degradation were evaluated, and all of the materials display excellent photocatalytic activity and stability. The calixarene-Ti coordination is therefore an effective strategy to enlarge the visible-light absorption band of Ti-O materials and improve their photoelectric/photocatalytic performances.

Screening of Beauveria bassiana with high biocontrol potential based on ARTP mutagenesis and high-throughput FACS.

Beauveria bassiana is a promising biocontrol agent due to its entomopathogenic activities and residue-free characteristics. However, its susceptibility to abiotic stresses and naturally low virulence limit the effective application of this fungus. To effectively obtain fungal strains with high biocontrol potential, fluorescence-activated cell sorting (FACS) was used to screen mutant libraries generated by atmospheric and room temperature plasma (ARTP). Among about 8000 mutants obtained by ARTP mutagenesis, six candidate mutants were selected according to the forward scatter (FSC) signal readings of FACS. B6, with a 37.4% higher FSC reading than wild-type (WT), showed a 32.6% increase in virulence. It also presented a 13.5% decrease in median germinating time (GT50) and a 12.1% increase in blastospore production. Comparative analysis between insect transcriptional responses to B6 and WT infection showed that the immune response coupled with protein digestion and absorption progress was highly activated in B6-infected Galleria mellonella larvae, while fatty acid synthesis was suppressed after 3 days of infection. Our results confirmed the feasibility of sorting B. bassiana with high biocontrol potential via the combination of ARTP and FACS and facilitated the understanding of insect-pathogen interactions, highlighting a new strategy for modifying entomopathogenic fungi to improve the efficiency of biological control.

Characterization of trehalose-6-phosphate phosphatase in trehalose biosynthesis, asexual development, stress resistance and virulence of an insect mycopathogen.

Biological control potential of entomopathogenic fungi depending on conidiation capacity, conidial stress tolerance and virulence can be improved through genetic engineering. To explore a possible role of trehalose biosynthesis pathway in improving fungal pest-control potential, we characterized biological functions of trehalose-6-phosphate phosphatase (BbTPP) in Beauveria bassiana, an insect mycopathogen that serves as a main source of fungal insecticides. Deletion of BbTPP resulted in abolished trehalose biosynthesis, reduced conidiation capacity, decreases in conidial thermotolerance and UV-B resistance, increased hyphal sensitivities to chemical stresses, and attenuated virulence. By contrast, over-expression of BbTPP led to increased trehalose accumulation, decreased T6P accumulation, and enhanced stress tolerance and virulence despite little impact on growth and conidiation under normal conditions. These results indicate that BbTPP serves as not only a key player in control of trehalose biosynthesis required for multiple cellular functions but also a potential candidate to be exploited for genetic improvement of fungal potential against insect pests.

Construction of a novel cell-trappable fluorescent probe for hydrogen sulfide (H2S) and its bio-imaging application.

Fluorescence detection of H2S in living organisms is greatly advantageous because it is nondestructive and can be used for in situ analysis. We have constructed a novel rhodamine analogue dye (Rho630) by extending the conjugated system of rhodamine to create a novel cell-trappable H2S fluorescent probe Rho630-AM-H2S with red light emission. Its application for H2S fluorescence detection in living HeLa cells and zebrafish was investigated. As expected, Rho630-AM-H2S showed a huge fluorescence turn-on response of about 20-fold at 630 nm and good selectivity toward H2S in solution. An MTT assay demonstrated that the probe showed negligible cytotoxicity in the concentrations typically used in fluorescence imaging experiments. Cell imaging experiments revealed that compared with compound 4 without cell-trappable unit modification, Rho630-AM-H2S exhibited remarkably enhanced cell penetration ability, as an enormous fluorescence signal increase was observed at the red channel within 5 min after Rho630-AM-H2S was incubated with HeLa cells. Finally, the probe Rho630-AM-H2S was used to detect H2S in living HeLa cells and zebrafish with great fluorescence enhancement in the red channel. Graphical abstract.

Cationic High Molecular Weight Lignin Polymer: A Flocculant for the Removal of Anionic Azo-Dyes from Simulated Wastewater.

The presence of dyes in wastewater effluents made from the textile industry is a major environmental problem due to their complex structure and poor biodegradability. In this study, a cationic lignin polymer was synthesized via the free radical polymerization of lignin with [2-(methacryloyloxy) ethyl] trimethyl ammonium chloride (METAC) and used to remove anionic azo-dyes (reactive black 5, RB5, and reactive orange 16, RO16) from simulated wastewater. The effects of pH, salt, and concentration of dyes, as well as the charge density and molecular weight of lignin-METAC polymer on dye removal were examined. Results demonstrated that lignin-METAC was an effective flocculant for the removal of dye via charge neutralization and bridging mechanisms. The dye removal efficiency of lignin-METAC polymer was independent of pH. The dosage of the lignin polymer required for reaching the maximum removal had a linear relationship with the dye concentration. The presence of inorganic salts including NaCl, NaNO3, and Na2SO4 had a marginal effect on the dye removal. Under the optimized conditions, greater than 98% of RB5 and 94% of RO16 were removed at lignin-METAC concentrations of 120 mg/L and 105 mg/L in the dye solutions, respectively.

In situ facile synthesis and antibacterial activity of Ag-MOFs/cellulose filter paper composites for fruit fresh-keeping.

A large number of fresh fruits are wasted in the supply chain due to spoilage, so it is crucial to develop fruit preservation materials. Herein, two novel Ag-MOFs/carboxymethyl filter paper (Ag-MOFs/CMFP) composites were successfully synthesized by in situ facile synthesis, which can be used as packaging materials to delay fruit spoilage. The synthesis process is simple and environmentally friendly, and the reaction conditions are mild. The mechanical property, water stability, and antibacterial activity of the as-synthesized Ag-MOFs/CMFP composites were investigated. Specifically, the composites exhibited high mechanical performance and the tensile strength was >10.00 MPa. Moreover, the composites displayed good water stability and can remain stable in water environment for >7 days, which can be attributed to the strong interaction between Ag-MOFs and CMFP. Significantly, Ag-MOF particles endow the composite papers with excellent antibacterial activity, which can inactivate 99.9 % of the bacteria. Attributed to these characteristics, these composite papers were used as fruit fresh-keeping materials and can prolong the shelf-life of cherry tomatoes and peaches for >10 days. This research not only provides a facile synthesis strategy for the flexible MOFs paper, but also provides instructive guidance for related research on fruit preservation materials.

Strategies for measuring concentrations and forms of amyloid-ß peptides.

Alzheimer's disease (AD) is affecting more and more people worldwide without the effective treatment, while the existed pathological mechanism has been confirmed barely useful in the treatment. Amyloid-ß peptide (Aß), a main component of senile plaque, is regarded as the most promising target in AD treatment. Aß clearance from AD brain seems to be a reliably therapeutic strategy, as the two exited drugs, GV-971 and aducanumab, are both developed based on it. However, doubt still exists. To exhaustive expound on the pathological mechanism of Aß, rigorous analyses on the concentrations and aggregation forms are essential. Thus, it is attracting broad attention these years. However, most of the sensors have not been used in pathological studies, as the lack of the bridge between analytical chemist and pathologists. In this review, we made a brief introduce on Aß-related pathological mechanism included in ß-amyloid hypothesis to elucidate the detection conditions of sensor methods. Furthermore, a summary of the sensor methods was made, which were based on Aß concentrations and form detections that have been developed in the past 10 years. As the greatest number of the sensors were built on fluorescent spectroscopy, electrochemistry, and Roman spectroscopy, detailed elucidation on them was made. Notably, the aggregation process is another important factor in revealing the progress of AD and developing the treatment methods, so the sensors on monitoring Aß aggregation processes were also summarized.

A fluorescent probe for detecting H2O2 and delivering H2S in lysosomes and its application in maintaining the redox environments.

Hydrogen peroxide (H2O2) is a reactive oxygen species (ROS) that can be used as a marker for the occurrence of oxidative stress in the organism. Lysosomes serve as intracellular digestive sites, and when the concentration of H2O2 in them is abnormal, lysosomal function is often impaired, leading to the development of diseases. Hydrogen sulfide (H2S) acts as a gaseous signaling molecule that scavenges H2O2 from cells and tissues, thereby maintaining the redox environment of the body. However, most of the reported hydrogen peroxide fluorescent probes so far can only detect H2O2, but cannot maintain the intracellular redox environment. In this paper, an H2O2 fluorescent probe LN-HOD with lysosomal targeting properties was designed and synthesized by combining the H2O2 recognition site with a naphthylamine fluorophore via a thiocarbamate moiety. The probe has the advantages of large Stokes shift (110 nm), high sensitivity and good H2S release capability. The probe LN-HOD can be used to detect H2O2 in cells, zebrafish and plant roots. In addition, LN-HOD detects changes in the concentration of H2O2 in plant roots when Arabidopsis is stressed by cadmium ion (Cd2+). And through its ability to release H2S, it can help to remove excess H2O2 and maintain the redox environment in cells, zebrafish and plant roots. The present work provides new ideas for the detection and assisted removal of H2O2, which contributes to the in-depth study of the cellular microenvironment in organisms.

Eco-friendly food packaging based on paper coated with a bio-based antibacterial coating composed of carbamate starch, calcium lignosulfonate, cellulose nanofibrils, and silver nanoparticles.

Traditional paper-based packaging commonly needs to be coated to achieve sufficient mechanical and barrier performances. In this research, a bio-based coating for paper was developed from carbamate starch (Sc), calcium lignosulfonate (CL), and cellulose nanofibrils (CNF). Controlling the electrostatic and hydrogen-bonding interactions among the components of the coating was conducive to tailoring the structure and performance of the coated paper. When the degree of substitution (Ds) of Sc was 0.10, the amount of CL was 1.00 g, and the amount of CNF was 0.65 % of the weight of Sc, the paper coated with the resulting 0.10Sc-1.00CL-0.65CNF coating exhibited increased hydrophobicity and excellent mechanical, air-barrier, and UV-light-barrier properties. After the addition of 0.10 % of silver nano-particles (AgNPs) to the 0.10Sc-1.00CL-0.65CNF coating, the paper coated with the resulting 0.10Sc-1.00CL-0.65CNF-0.10AgNPs coating exhibited good antibacterial activity against Escherichia coli and Staphylococcus aureus. The coated paper was used as the packaging for cherry tomatoes stored under ambient conditions. Due to the synergistic preservation effects of the Sc-CL-CNF coating and AgNPs, the shelf life of the cherry tomatoes was at least 7 days. The coated paper described herein has the potential for applications in the food packaging sector.

N-lauric-O-carboxymethyl chitosan: Synthesis, characterization and application as a pH-responsive carrier for curcumin particles.

A pH-responsive amphiphilic chitosan derivative, N-lauric-O-carboxymethyl chitosan (LA-CMCh), is synthesized. Its molecular structures are characterized by FTIR, 1H NMR, and XRD methods. The influencing factors are investigated, including the amount of lauric acid (LA), carboxymethyl chitosan (CMCh), N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC), and N-hydroxysuccinimide (NHS), and their molar ratio, reaction time, and reaction temperature on the substitution. The degrees of substitution (DS) of the lauric groups on the -NH2 groups are calculated based on the integrated data of 1H NMR spectra. The optimum reaction condition is obtained as a reaction time of 6 h, a reaction temperature of 80 °C, and a molar ratio of lauric acid to O-carboxymethyl chitosan to N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride to N-hydroxysuccinimide of 1:3:4.5:4.5, respectively. The crystallinity and initial decomposition temperature of LA-CMCh decrease, but the maximum decomposition temperature increases. The crystallinity is reduced due to the introduction of LA and the degree of hydrogen bonding among LA-CMCh molecules. LA-CMCh could self-aggregate into particles, which size and critical aggregation concentration depend on the degree of substitution and medium pH. LA-CMCh aggregates could load curcumin up to 21.70 %, and continuously release curcumin for >200 min. LA-CMCh shows nontoxicity to fibroblast HFF-1 cells and good antibacterial activity against S. aureus and E. coli, indicating that it could be used as an oil-soluble-drug carrier.

Halloysite nanotubes enhanced polyimide/oxidized-lignin nanofiber separators for long-cycling lithium metal batteries.

The high energy density and robust cycle properties of lithium-ion batteries contribute to their extensive range of applications. Polyolefin separators are often used for the purpose of storing electrolytes, hence ensuring the efficient internal ion transport. Nevertheless, the electrochemical performance of lithium-ion batteries is constrained by its limited interaction with electrolytes and poor capacity for cation transport. This work presents the preparation of a new bio-based nanofiber separator by combining oxidized lignin (OL) and halloysite nanotubes (HNTs) with polyimide (PI) using an electrospinning technique. Analysis was conducted to examine and compare the structure, morphology, thermal characteristics, and EIS of the separator with those of commercially available polypropylene separator (PP). The results indicate that the PI@OL and PI-OL@ 10 % HNTs separators exhibit higher lithium ion transference number and ionic conductivity. Moreover, the use of HNTs successfully impeded the proliferation of lithium dendrites, hence exerting a beneficial impact on both the cycle performance and multiplier performance of the battery. Consequently, after undergoing 300 iterations, the battery capacity of LiFePO4|PI-OL@ 10 % HNTs|Li stays at 92.1 %, surpassing that of PP (86.8 %) and PI@OL (89.6 %). These findings indicate that this new bio-based battery separator (PI-OL@HNTs) has the great potential to serve as a substitute for the commonly used PP separator in lithium metal batteries.

Gelatin/carboxymethyl chitosan/aloe juice hydrogels with skin-like endurance and quick recovery: Preparation, characterization, and properties.

Gelatin-based hydrogels have gained considerable attention due to their resemblance to the extracellular matrix and hydrophilic three-dimensional network structure. Apart from providing an air-permeable and moist environment, these hydrogels optimize the inflammatory microenvironment of the wounds. These properties make gelatin-based hydrogels highly competitive in the field of wound dressings. In this study, a series of composite hydrogels were prepared using gelatin (Gel) and carboxymethyl chitosan (CMCh) as primary materials, glutaraldehyde as a crosslinker, and aloe vera juice as an anti-inflammatory component. The properties of the hydrogel, including its rheological properties, microscopic structures, mechanical properties, swelling ratios, thermal stability, antibacterial properties, and biocompatibility, were investigated. The results demonstrate that the gelatin-based hydrogels exhibit good elasticity and rapid self-healing ability. The hydrogels exhibited slight shear behavior, which is advantageous for skin care applications. Furthermore, the inclusion of aloe vera juice into the hydrogel resulted in a dense structure, improved mechanical properties and enhanced swelling ratio. The Gel/CMCh/Aloe hydrogels tolerate a compressive strength similar to that of human skin. Moreover, the hydrogels displayed excellent cytocompatibility with HFF-1 cells, and exhibited antibacterial activity against E. coli and S. aureus. Lomefloxacin was used as a model drug to study the releasing behavior of the Gel/CMCh/aloe hydrogels. The results showed that the drug was released rapidly at the initial stage, and could continue to be released for 12 h, the maximum releasing rate exceeded 20 %. These findings suggest that the gelatin-based hydrogels hold great promise as effective wound dressings.

Guluronic acid can inhibit copper(II) and amyloid - ß peptide coordination and reduce copper-related reactive oxygen species formation associated with Alzheimer's disease.

Copper-related reactive oxygen species (ROS) formation can lead to neuropathologic degradation associated with Alzheimer's disease (AD) according to amyloid cascade hypothesis. A complexing agent that can selectively chelate with copper ions and capture copper ions from the complex formed by copper ions and amyloid-ß (Cu - Aß complex) may be available in reducing ROS formation. Herein, we described applications of guluronic acid (GA), a natural oligosaccharide complexing agent obtained from enzymatic hydrolysis of brown algae, in reducing copper-related ROS formation. UV-vis absorption spectra demonstrated the coordination between GA and Cu(II). Ascorbic acid consumption and coumarin-3-carboxylic acid fluorescence assays confirmed the viability of GA in reducing ROS formation in solutions containing other metal ions and Aß. Fluorescence kinetics, DPPH radical clearance and high resolution X - ray photoelectron spectroscopy results revealed the reductivity of GA. Human liver hepatocellular carcinoma (HepG2) cell viability demonstrated the biocompatibility of GA at concentrations lower than 320 µM. Cytotoxic results of human neuroblastoma (SH-SY5Y) cells verified that GA can inhibit copper-related ROS damage in neuronal cells. Our findings, combined with the advantages of marine drugs, make GA a promising candidate in reducing copper-related ROS formation associated with AD therapy.

Influence of structure and functional group of modified kraft lignin on adsorption behavior of dye.

Utilization of kraft lignin to produce bio-based adsorptive material for effective dye adsorption from industrial wastewater is essential to fulfilling the significant environmental protection needs. Lignin is the most abundant byproduct material with a chemical structure containing various functional groups. However, the complicated chemical structure makes it somewhat hydrophobic and incompatible, which limits its direct application as an adsorption material. Chemical modification is a common way to enhance lignin properties. In this work, the kraft lignin was modified through direct amination using Mannich reaction and oxidization followed by amination as new route of lignin modification. The prepared lignins, including aminated lignin (AL), oxidized lignin (OL), and aminated-oxidized lignin (AOL), as well as unmodified kraft lignin, were analyzed by Fourier transform infrared spectroscopy (FT-IR), thermogravimetric analysis (TGA), scanning electron microscope (SEM), X-ray photoelectron spectroscopy (XPS), elemental analysis and 1H-nuclear magnetic resonance measurements (1HNMR). The adsorption behaviors of modified lignins for the malachite green in aqueous solution were investigated well and discussed, as well as the adsorption kinetics and thermodynamic equations. Compared with other aminated lignin (AL), the AOL displayed a high adsorption capacity of 99.1 % dye removal, due to its more effective functional groups. The change in structure and functional groups on the lignin molecules during oxidation and amination had no effect on its adsorption mechanisms. The adsorption process of malachite green on different kinds of lignin belongs to endothermic chemical adsorption, which mainly consists of monolayer adsorption. The modification of lignin through oxidation followed by amination process, afforded kraft lignin a broad potential application in the field of wastewater treatment.

A highly sensitive and low toxicity cellulose-based fluorescent polymer for H2S detection in cells, zebrafish and food samples.

A cellulose based polymer probe (HC-HS) was prepared for the detection of H2S. HC-HS can be applied to fluorescence imaging of H2S in living cells and zebrafish, and HC-HS was made into test strips to detect H2S produced in the process of food corruption.

A novel high-performance electrospun of polyimide/lignin nanofibers with unique electrochemical properties and its application as lithium-ion batteries separators.

Polypropylene is currently one of the most widely used separators in lithium batteries because of its low cost and chemical stability. However, it also has some intrinsic flaws that hamper the battery performance, such as poor wettability, low ionic conductivity, and some safety issues. This work introduces a novel electrospun nanofibrous consisting of polyimide (PI) blended with lignin (L) to serve as a new class of bio-based separators for lithium-ion batteries. The morphology and properties of the prepared membranes were studied in detail and compared with those of a commercial polypropylene separator. Interestingly, the polar groups in lignin promoted the affinity to the electrolytes and improved the liquid absorption properties of the PI-L membrane. Besides, the PI-L separator showed a higher ionic conductivity (1.78 × 10-3 S/cm) and Li+ transference number (0.787). Furthermore, the battery's cycle and rate performance improved due to adding of lignin. The capacity retention of the assembled LiFePO4 | PI-L | Li Battery was 95.1 % after 100 cycles at 1C current density, which was higher than that of the PP (90 %). Based on the results, PI-L, a bio-based battery separator, can potentially replace the current PP separators in lithium metal batteries.

3D Printing of Solar Crystallizer with Polylactic Acid/Carbon Composites for Zero Liquid Discharge of High-Salinity Brine.

Zero liquid discharge (ZLD) is a technique for treating high-salinity brine to obtain freshwater and/or salt using a solar interface evaporator. However, salt accumulation on the surface of the evaporator is a big challenge to maintaining stable water evaporation. In this study, a simple and easy-to-manufacture evaporator, also called a crystallizer, was designed and fabricated by 3D printing. The photothermal layer printed with polylactic acid/carbon composites had acceptable light absorption (93%) within the wavelength zone of 250 nm-2500 nm. The micron-sized voids formed during 3D printing provided abundant water transportation channels inside the crystallizer. After surface hydrophilic modification, the crystallizer had an ultra-hydrophilic channel structure and gravity-assisted salt recovery function. The results revealed that the angles between the photothermal layers affected the efficacy of solar evaporation and the yield of solid salt. The crystallizer with the angle of 90° between two photothermal layers could collect more solid salt than the three other designs with angles of 30°, 60°, and 120°, respectively. The crystallizer has high evaporation and salt crystallization efficiency in a high-salinity brine environment, which is expected to have application potentials in the zero liquid discharge of wastewater and valuable salt recovery.

MXene/bacterial cellulose/Fe3O4/methyltrimethoxylsilane flexible film with hydrophobic for effective electromagnetic shielding.

Electromagnetic (EM) pollution has become a serious problem in modern society as it affects human lives. The fabrication of strong and highly flexible materials for electromagnetic interference (EMI) shielding applications is extremely urgent. Herein, a MXene Ti3C2Tx/Fe3O4 & bacterial cellulose (BC)/Fe3O4&Methyltrimethoxysilane (MTMS) flexible hydrophobic electromagnetic shielding film (SBTFX-Y, X and Y were the number of layers of BC/Fe3O4 and the layers of Ti3C2Tx/Fe3O4), was fabricated. In the prepared film, MXene Ti3C2Tx absorbs a large amount of radio waves through polarization relaxation and conduction loss. Because of its extremely low reflectance of electromagnetic waves, BC@Fe3O4, as the outermost layer of the material, allows more electromagnetic waves to incident inside the material. The maximum electromagnetic interference (EMI) shielding efficiency (SE) of 68 dB was achieved for the composite film at 45 µm thickness. What's more, the SBTFX-Y films show excellent mechanical properties, hydrophobicity and flexibility. The unique stratified structure of the film provides a new strategy for designing high-performance EMI shielding films with excellent surface and mechanical properties.