Circulating white blood cell traits and prolonged night shifts: a cross-sectional study based on nurses in Guangxi.

This study aimed to elucidate the effects of long day and night shifts on immune cells in a population of nurses. This cross-sectional study in December 2019 was based on a group of nurses. 1568 physically healthy caregivers were included, including 1540 women and 28 men. 1093 nurses had long-term shift work (working in a rotating system for > 1 year). The receiver operating characteristic curve, Ensemble Learning, and Logistic regression analyses were used to evaluate factors related to long-term shift work. The night shift group nurses had significantly higher MPV, PLCR, and WBC and significantly lower BASO%, ELR, MCHC, PLR, RDW-CV, and RDW-SD (P < 0.01). ROC curves showed that WBC, PLR, ELR, RDW_CV, and BASO% were more related to the night shift. Ensemble Learning, combined with the LASSO model, finally filtered out three indicators of night shifts related to ELR, WBC, and RDW_SD. Finally, logistic regression analysis showed that the nurses' night shift situation greatly influenced two peripheral blood ELR and WBC indicators (ELR: log (OR) = - 3.9, 95% CI: - 5.8- - 2.0; WBC: log (OR) = 0.25, 95% CI: 0.18-0.32). Finally, we showed that, unlike WBC, the relative riskiness of ELR showed opposite results among junior nurses and middle-senior nurses (log (OR) 6.5 (95% CI: 1.2, 13) and - 7.1 (95% CI: - 10, - 3.8), respectively). Our study found that prolonged night shifts were associated with abnormal WBC and ELR, but after strict age matching, WBC remained significantly different. These findings help to confirm that COVID-19 and tumorigenesis (e.g., breast cancer) are significantly associated with circadian rhythm disruption. However, more detailed studies are needed to confirm this.

Numerical Solution of the Electric Field and Dielectrophoresis Force of Electrostatic Traveling Wave System.

Electrostatic traveling wave (ETW) methods have shown promising performance in dust mitigation of solar panels, particle transport and separation in in situ space resource utilization, cell manipulation, and separation in biology. The ETW field distribution is required to analyze the forces applied to particles and to evaluate ETW design parameters. This study presents the numerical results of the ETW field distribution generated by a parallel electrode array using both the charge simulation method (CSM) and the boundary element method (BEM). A low accumulated error of the CSM is achieved by properly arranging the positions and numbers of contour points and fictitious charges. The BEM can avoid the inconvenience of the charge position required in the CSM. The numerical results show extremely close agreement between the CSM and BEM. For simplification, the method of images is introduced in the implementation of the CSM and BEM. Moreover, analytical formulas are obtained for the integral of Green's function along boundary elements. For further validation, the results are cross-checked using the finite element method (FEM). It is found that discrepancies occur at the ends of the electrode array. Finally, analyses are provided of the electric field and dielectrophoretic (DEP) components. Emphasis is given to the regions close to the electrode surfaces. These results provide guidance for the fabrication of ETW systems for various applications.

Peripheral blood lipid and liver and kidney function test results in long-term night shift nurses: a cross-sectional study in South China.

Purpose: This study aimed to elucidate the effects of long-term day and night shifts on liver function and lipid metabolism in a group of nurses. Methods: This cross-sectional study in December 2019 was based on a group of nurses. A total of 1,253 physically healthy caregivers were included, including 1231 women and 22 men. A total of 886 nurses had long-term shift work (working in a rotating system for >1 year). The receiver operating characteristic (ROC) curve and logistic regression analyses were used to evaluate factors related to long-term shift work. Results: We observed differences in liver and kidney indicators between the non-night and night shift groups. The ROC curve revealed that CHO (AUC: 62.4%), LDLC (AUC: 62%), and GLUO (AUC: 61.5%) were more related to the night shift. Logistic regression analysis showed that night shift work was associated significantly with CREA (log (OR) = -0.02, 95% CI: -0.04 to -0.01), CHO (log (OR) = -0.38, 95% CI: -0.67 to -0.09), and GLUO (log (OR) = -0.35, 95% CI: -0.56 to -0.17). This correlation was observed only for CHO and LDHC (CHO: log (OR) = -0.55, 95% CI: -0.98 to -0.12; LDLC: log (OR) = 0.83, 95% CI: 0.32, 1.4) after age standardization. After using propensity score matching, we did not find evidence to support that the indicators differed between night and non-night shift groups. Conclusion: Our study observed an association of long-term night work with abnormal liver and kidney function and dyslipidemia, but the difference was not significant after strict age matching. Although these findings may support interventions for long-term night shift nurses, more detailed studies are needed to confirm.

Lithospheric delamination and upwelling asthenosphere in the Longmenshan area: insight from teleseismic P-wave tomography.

We apply teleseismic P-wave tomography to reconstruct the velocity structure of the Longmenshan area. Our results show possible large-scale delamination beneath the Songpan-Ganzi and Qiangtang terranes, which induced upwelling asthenosphere. Upwelling asthenosphere might have led to lower crust heating, facilitating eastward extrusion of the Songpan Ganzi terrane resulting in localized deformation and uplift along the Longmenshan orogenic belt. We suggest that the eastward extrusion of the Songpan-Ganzi terrane against the rigid lithospheric root of the Sichuan Basin results in stress accumulation and release, leading to large earthquakes in the Longmenshan area.

Seismic waveform simulation for models with fluctuating interfaces.

The contrast of elastic properties across a subsurface interface imposes a dominant influence on the seismic wavefield, which includes transmitted and reflected waves from the interface. Therefore, for an accurate waveform simulation, it is necessary to have an accurate representation of the subsurface interfaces within the numerical model. Accordingly, body-fitted gridding is used to partition subsurface models so that the grids coincide well with both the irregular surface and fluctuating interfaces of the Earth. However, non-rectangular meshes inevitably exist across fluctuating interfaces. This non-orthogonality degrades the accuracy of the waveform simulation when using a conventional finite-difference method. Here, we find that a summation-by-parts (SBP) finite-difference method can be used for models with non-rectangular meshes across fluctuating interfaces, and can achieve desirable simulation accuracy. The acute angle of non-rectangular meshes can be relaxed to as low as 47°. The cell size rate of change between neighbouring grids can be relaxed to as much as 30%. Because the non-orthogonality of grids has a much smaller impact on the waveform simulation accuracy, the model discretisation can be relatively flexible for fitting fluctuating boundaries within any complex problem. Consequently, seismic waveform inversion can explicitly include fluctuating interfaces within a subsurface velocity model.

Seismic waveform tomography with shot-encoding using a restarted L-BFGS algorithm.

In seismic waveform tomography, or full-waveform inversion (FWI), one effective strategy used to reduce the computational cost is shot-encoding, which encodes all shots randomly and sums them into one super shot to significantly reduce the number of wavefield simulations in the inversion. However, this process will induce instability in the iterative inversion regardless of whether it uses a robust limited-memory BFGS (L-BFGS) algorithm. The restarted L-BFGS algorithm proposed here is both stable and efficient. This breakthrough ensures, for the first time, the applicability of advanced FWI methods to three-dimensional seismic field data. In a standard L-BFGS algorithm, if the shot-encoding remains unchanged, it will generate a crosstalk effect between different shots. This crosstalk effect can only be suppressed by employing sufficient randomness in the shot-encoding. Therefore, the implementation of the L-BFGS algorithm is restarted at every segment. Each segment consists of a number of iterations; the first few iterations use an invariant encoding, while the remainder use random re-coding. This restarted L-BFGS algorithm balances the computational efficiency of shot-encoding, the convergence stability of the L-BFGS algorithm, and the inversion quality characteristic of random encoding in FWI.

Vertical crustal motions across Eastern Tibet revealed by topography-dependent seismic tomography.

Using a topography-dependent tomographic scheme, the seismic velocity structure of the Eastern Tibetan Plateau, including the uplifted Longmenshan (LMS) orogenic belt, is accurately imaged in spite of the extreme topographic relief in the LMS region and thick sedimentary covers in the neighbouring Sichuan Basin. The obtained image shows a high-resolution upper crustal structure on a 500 km-long profile that is perpendicular to the LMS. The image clearly shows that the crystalline basement was uplifted within the LMS orogenic belt, and that the neighbouring Songpan-Ganzi Terrane was covered by a thick flysch belt, with evidence of near-surface thrust faults caused by convergence between Eastern Tibet and the Sichuan Basin. The indication that the lower crust beneath the LMS was folded and pushed upwards and the upper crust was removed by exhumation, supports the concept of a lower crustal channel flow beneath Eastern Tibet. The image also reveals that the destructive Wenchuan earthquake of year 2008 occurred in the upper crust, directly at the structural discontinuity between Eastern Tibet Plateau and the Sichuan Basin.

Fibroblasts in Head and Neck Squamous Cell Carcinoma Associated With Perineural Invasion Have High-Level Nuclear Yes-Associated Protein (YAP) Expression.

We retrospectively studied the expression of Yes-associated protein (YAP) using immunohistochemical staining in 10 cases of head and neck squamous cell carcinoma with associated perineural invasion. We find that fibroblasts in areas associated with perineural invasion show higher levels of nuclear YAP compared to fibroblasts in the stroma of normal mucosa, with a median cell count of 35.4 per high-power field in the former and 3.9 in the latter. No differences were observed between the expression of YAP phosphorylated at Ser127 in the tumoral stroma compared to that in the normal mucosa, with a median cell count expression of 4.9 in the former versus 5.0 in the latter. Therefore, a strong and increased nuclear YAP expression in fibroblasts associated with perineural invasion in head and neck squamous cell carcinoma suggests that YAP-mediated transcription programs in these fibroblasts may contribute to perineural invasion.