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1.
Public Health Nurs ; 41(2): 233-244, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38111292

RESUMO

OBJECTIVES: Despite receiving cardiopulmonary resuscitation (CPR) training, over 50% of bystanders were unable to actually perform CPR. Understanding public willingness and attitudes toward bystander CPR is crucial in explaining whether people initiate CPR. This study aimed to develop a theoretical understanding of factors that influence the public's willingness and attitudes to perform CPR. DESIGN: This was a qualitative study using the grounded theory method. METHODS: The data were collected from semi-structured interviews with 28 participants between August 2022 and November 2022. Purposive sampling and theoretical sampling were used to recruit participants. Interviews were recorded and transcribed. Data were analyzed using open, axial, and selective coding. RESULTS: Nine categories and 24 subcategories were summarized from four aspects: willingness, attitudes, implementation, and training. Willingness included self-willingness, self-perception, and societal factors; attitudes covered personality traits, reactions to patients and environment; implementation comprised knowledge and skills, situational coping, and risk perception; training included CPR training accessibility and barriers to CPR training. A theoretical framework of public CPR willingness, attitudes, and their influencing factors was developed. CONCLUSION: The public's CPR willingness, attitudes, training, and implementation were interrelated and influential. The findings may have significant implications for the development of legislation and policy related to CPR popularization and training.


Assuntos
Reanimação Cardiopulmonar , Parada Cardíaca Extra-Hospitalar , Humanos , Reanimação Cardiopulmonar/educação , Reanimação Cardiopulmonar/métodos , Teoria Fundamentada , Conhecimentos, Atitudes e Prática em Saúde , Inquéritos e Questionários , Pesquisa Qualitativa , Parada Cardíaca Extra-Hospitalar/terapia
2.
J Am Psychiatr Nurses Assoc ; 25(4): 305-313, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30295107

RESUMO

INTRODUCTION: There is considerable need for effective and accessible treatment for opioid use disorder. AIMS: Our study explored differences in buprenorphine treatment retention and duration, with a focus on selected sociodemographic factors and treatment indicators, in two different settings: an office-based buprenorphine induction and stabilization clinic (OBIC) and a community-based primary care clinic (CPC). METHOD: This nonexperimental retrospective chart review compared demographic information and buprenorphine treatment details, including treatment retention and duration. RESULTS: There were no statistically significant differences in buprenorphine treatment indicators between the OBIC and CPC groups, with two exceptions: the number of written buprenorphine prescriptions was significantly greater for the OBIC group, as was the number of filled buprenorphine prescriptions. CONCLUSIONS: Given similar treatment retention and duration in two different buprenorphine treatment settings, our findings suggest that access to buprenorphine treatment in standard integrated care settings can be supplemented by novel treatment structures such as the OBIC in order to increase access to care during the current opioid epidemic.


Assuntos
Analgésicos Opioides/uso terapêutico , Buprenorfina/uso terapêutico , Acessibilidade aos Serviços de Saúde/estatística & dados numéricos , Transtornos Relacionados ao Uso de Opioides/tratamento farmacológico , Retenção nos Cuidados/estatística & dados numéricos , Adulto , Feminino , Humanos , Masculino , Estudos Retrospectivos , Resultado do Tratamento , Adulto Jovem
3.
J Nurs Scholarsh ; 50(2): 163-171, 2018 03.
Artigo em Inglês | MEDLINE | ID: mdl-29193636

RESUMO

PURPOSE: Displacement is traumatic and often an unwanted social change that can lead to a loss of identity and socioeconomic, physical, and psychological livelihood. The purpose of the study was to describe the association of perceived health and right-of-return hopefulness to depressive symptom severity in Palestinian refugees, taking into consideration gender and poverty. DESIGN: The descriptive, correlational study was framed within a socioecological lens. Data collection occurred between October 2015 and November 2015 in Amman, Jordan, which hosts the most Palestinian refugees in the world. The participants in the sample (N = 177) had a mean age of 36.9 years. METHODS: Participants responded to the Patient Health Questionnaire for depressive symptom severity, the RAND-36 perceived health item, and a statement about hopefulness to return to Palestine. Descriptive, correlation, and logistic and linear regression analyses were computed. FINDINGS: Results showed that 43% of participants had moderate to severe depressive symptoms, 42% lived in poverty, and 20% had fair or poor health; yet, 60% were hopeful about returning to Palestine. Participants who had better perceived health and right-of-return hopefulness were less likely to have symptoms of major depression. Perceived health was the only factor-not gender, poverty, or right-of-return hopefulness-that explained the variance in depressive symptoms. CONCLUSIONS: Palestinian adult refugees in Jordan exhibited symptoms of major depression that were associated with poorer perceived health and less hopefulness about repatriation to Palestine. CLINICAL RELEVANCE: Nurses with community or mental health specialization can play a major role by systematically screening refugees for depression using worldwide, evidence-based tools and by advocating for policies that can improve the health and living conditions of refugees.


Assuntos
Árabes , Depressão/diagnóstico , Pobreza , Refugiados , Adulto , Estudos Transversais , Depressão/etiologia , Feminino , Nível de Saúde , Esperança , Humanos , Jordânia , Masculino , Pessoa de Meia-Idade , Sociologia , Inquéritos e Questionários
4.
Am J Public Health ; 112(S3): S268, 2022 06.
Artigo em Inglês | MEDLINE | ID: mdl-35679557
8.
Matern Child Health J ; 21(4): 863-872, 2017 04.
Artigo em Inglês | MEDLINE | ID: mdl-27565664

RESUMO

Background While breast milk is considered the gold standard of infant feeding, a majority of African American mothers are not exclusively breastfeeding their newborn infants. Objective The overall goal of this critical ethnographic research study was to describe infant feeding perceptions and experiences of African American mothers and their support persons. Methods Twenty-two participants (14 pregnant women and eight support persons) were recruited from public health programs and community based organizations in northern California. Data were collected through field observations, demographic questionnaires, and multiple in-person interviews. Thematic analysis was used to identify key themes. Results Half of the mothers noted an intention to exclusively breastfeed during the antepartum period. However, few mothers exclusively breastfed during the postpartum period. Many participants expressed guilt and shame for not being able to accomplish their antepartum goals. Life experiences and stressors, lack of breastfeeding role models, limited experiences with breastfeeding and lactation, and changes to the family dynamic played a major role in the infant feeding decision making process and breastfeeding duration. Conclusions for Practice Our observations suggest that while exclusivity goals were not being met, a considerable proportion of African American women were breastfeeding. Future interventions geared towards this population should include social media interventions, messaging around combination feeding, and increased education for identified social support persons. Public health measures aimed at reducing the current infant feeding inequities would benefit by also incorporating more culturally inclusive messaging around breastfeeding and lactation.


Assuntos
Negro ou Afro-Americano/psicologia , Aleitamento Materno/etnologia , Aleitamento Materno/psicologia , Tomada de Decisões , Mães/psicologia , Apoio Social , Adulto , Negro ou Afro-Americano/estatística & dados numéricos , Aleitamento Materno/estatística & dados numéricos , California/etnologia , Feminino , Grupos Focais , Conhecimentos, Atitudes e Prática em Saúde/etnologia , Humanos , Recém-Nascido , Mães/estatística & dados numéricos , Gravidez
9.
Nurs Outlook ; 65(4): 436-443, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28622883

RESUMO

BACKGROUND: Jordan hosts more Palestinian refugees than any country in the world. Conditions under which people in a community live influence their health-related quality of life (HRQOL). PURPOSE: The purpose of this descriptive comparative cross-sectional study was to compare HRQOL of Palestinian refugees in Jordan who live inside camps with those who live outside camps. METHODS: Participants, recruited from inside the Baqa'a camp (n = 86) and the surrounding Abu Nsair community (n = 91), completed the World Health Organization Quality of Life Brief questionnaire. FINDINGS: There were disparities in education and social relations and environment HRQOL related to income and residency, but not gender, among refugees. CONCLUSION: Refugees living inside camps, particularly if poorer, fared worse than refugees living outside camps. Enhanced programs and policies may be needed to improve HRQOL, education, and socioeconomics for camp refugees. Nursing's perspective on refugee health could make an important contribution to humanitarian efforts and health diplomacy.


Assuntos
Árabes/psicologia , Qualidade de Vida/psicologia , Refugiados/psicologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Estudos Transversais , Feminino , Humanos , Jordânia , Masculino , Pessoa de Meia-Idade , Fatores Socioeconômicos , Inquéritos e Questionários
10.
Nurs Outlook ; 64(5): 440-9, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27390082

RESUMO

BACKGROUND: Individual health behaviors affect whether U.S. Air Force (USAF) service members are fit and ready to deploy. PURPOSE: The purpose of this study was to understand health behaviors of USAF members to guide future interventions to reduce cardiovascular risks. METHODS: A qualitative descriptive study was conducted with a purposive sample of 24 active duty USAF participants. Conventional content analysis was used to derive data-driven themes that were compared with the Health Promotion Model (HPM). DISCUSSION: Participants defined health in a multifactorial way that covered physical, emotional, and spiritual dimensions. The three themes that contributed to participants' health behaviors addressed: "who I am," "what works for me," and the USAF culture. There was a poor fit between findings as expressed by these participants and the HPM. CONCLUSION: Although these findings were derived from a sample of USAF participants, the findings have implications for members of other military services. The findings also have relevance for nurses and other providers within the civilian work environments who can promote health and wellness by integrating a client's personal history into a plan for developing and sustaining a healthy lifestyle.


Assuntos
Atitude Frente a Saúde , Comportamentos Relacionados com a Saúde , Promoção da Saúde/métodos , Militares/psicologia , Adulto , California , Feminino , Humanos , Masculino
11.
Res Nurs Health ; 36(4): 359-72, 2013 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-23606271

RESUMO

Chinese Americans demonstrate greater prevalence of diabetes than non-Hispanic whites and find standard diabetes care disregards their cultural health beliefs. Academic researchers and Chinatown agencies collaborated to culturally adapt and test an efficacious cognitive-behavioral intervention using community-based participatory research. Using a delayed-treatment repeated-measures design, 145 adult Chinese immigrants with Type 2 diabetes completed treatment. Immediate benefits of treatment were evident in the improvement (p < .05) in diabetes self-efficacy, diabetes knowledge, bicultural efficacy, family emotional and instrumental support, diabetes quality of life, and diabetes distress. Prolonged benefits were evident in all changed variables 2 months post-intervention. The CBPR approach enabled the development of a culturally acceptable, efficacious behavioral intervention, and provides a model for working with communities that demonstrate health disparities.


Assuntos
Asiático/psicologia , Pesquisa Participativa Baseada na Comunidade , Diabetes Mellitus Tipo 2/etnologia , Diabetes Mellitus Tipo 2/psicologia , Conhecimentos, Atitudes e Prática em Saúde , Aceitação pelo Paciente de Cuidados de Saúde/psicologia , Cooperação do Paciente/etnologia , Adaptação Psicológica , Adulto , Idoso , Idoso de 80 Anos ou mais , Atitude Frente a Saúde , Características Culturais , Diabetes Mellitus Tipo 2/terapia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Cooperação do Paciente/psicologia , Avaliação de Programas e Projetos de Saúde
12.
Health Care Women Int ; 34(2): 103-21, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23311905

RESUMO

Malawi women are in the ironic juxtaposition of being socially disempowered while, at the same time, thought to hold the key to shaping an effective community response to the HIV crisis. Based on this juxtaposition, a descriptive, qualitative study was conducted in Malawi and the United States where 26 participants from nongovernmental organizations (NGOs) and community-based organizations (CBOs) discussed the roles of Malawi women. Interviews were audiotaped, transcribed, and analyzed. We identified an improvement in women's economic status as the strongest factor in reducing gender inequities. Through providing stipends for rural Malawi women, one NGO created unintended changes in gender roles.


Assuntos
Identidade de Gênero , Infecções por HIV/prevenção & controle , Organizações sem Fins Lucrativos/organização & administração , Poder Psicológico , Instituições Filantrópicas de Saúde/organização & administração , Adulto , Idoso , Antropologia Cultural , Pesquisa Participativa Baseada na Comunidade , Características Culturais , Feminino , Infecções por HIV/etnologia , Humanos , Entrevistas como Assunto , Malaui , Masculino , Pessoa de Meia-Idade , Pesquisa Qualitativa , Meio Social , Fatores Socioeconômicos , Inquéritos e Questionários , Gravação em Fita , Estados Unidos
13.
Nurs Res ; 61(1): 66-9, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22166911

RESUMO

BACKGROUND: Accurate estimation of coronary heart disease (CHD) risk is requisite for effective primary prevention of the disease. The Framingham Risk Score is the most commonly used method for estimating 10-year risk for CHD in asymptomatic individuals. Further noninvasive tests of atherosclerosis are widely available and may be added to enhance risk estimation. However, the ability to combine different test results explicitly in a quantitative way is limited, and a substantial gap remains in identification of those at high risk for future CHD. OBJECTIVES: The aims of this paper are to present information about and examples of how to estimate 10-year risk of developing CHD with the Framingham Risk Score and to demonstrate how to combine two different test results with Bayes' theorem. METHOD: Bayes' theorem of conditional probability is presented as a method by which to combine two different test results in a quantitative way to better identify high-risk asymptomatic individuals. DISCUSSION: Applying Bayes' theorem will help nurses to better estimate CHD risk, leading to optimal intervention plans. This method of refining risk estimation is especially useful for individuals who would fall into an intermediate-risk category based on the Framingham Risk Score.


Assuntos
Teorema de Bayes , Doença das Coronárias/prevenção & controle , Medição de Risco/métodos , Adulto , Fatores Etários , Pressão Sanguínea , Colesterol/sangue , Humanos , Funções Verossimilhança , Fatores Sexuais , Fumar , Triglicerídeos/sangue
14.
Workplace Health Saf ; 70(2): 97-119, 2022 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-35014589

RESUMO

BACKGROUND: Many American workers spend over 7 hours a day at work in primarily sedentary office work. Physical activity is a key aspect of optimizing health and preventing disease; yet, 80% of American adults do not meet the recommended guidelines for physical activity. In this systematic review, the relationship between physical work environment and physical activity among office workers was explored. METHODS: Of the 321 studies screened, 26 studies met the eligibility criteria and were included for evaluation in this systematic review. RESULTS: Of the 26 studies, four were cross-sectional studies, 14 were quasi-experimental studies, and eight were randomized control trials. Physical activity during the workday was measured using self-report surveys and electromechanical devices such as accelerometers. Physical work environments examined by the studies included different types of desks (n = 16), office arrangements (n = 5), and building design (n = 5). In nine studies, office environments and building work environments designed to promote activity using active design principles such as stairs and flexible workspaces were associated with increased physical activity. Sit-stand desks reduced overall sitting time, but had a minimal effect on physical activity. CONCLUSION/APPLICATION TO PRACTICE: Offices and buildings designed for activity had the largest impact on physical activity among office workers. To increase physical activity in office workers, focus should be placed on opportunities to increase incidental movement that can increase physical activity throughout the workday. Occupational health nurses should advocate workspace designs that can increase physical activity in workers.


Assuntos
Saúde Ocupacional , Postura , Adulto , Exercício Físico , Humanos , Autorrelato , Local de Trabalho
15.
Public Health Nurs ; 27(6): 552-60, 2010.
Artigo em Inglês | MEDLINE | ID: mdl-21087309

RESUMO

OBJECTIVE: The purpose of this study was to understand the processes and interactions that African American tobacco control organizations use to engage African American communities in tobacco control efforts. DESIGN AND SAMPLE: The study used grounded theory methods to interpret participant's perspectives on tobacco control. The study sample consisted of African American tobacco control program directors from African American tobacco control organizations throughout the United States. MEASURES: Data collection involved 1 interview per participant using a semistructured interview at a location selected by the participant. Each interview lasted approximately 30-90 min. RESULTS: The results showed that organizations used specific strategies to involve African Americans in tobacco control. The tobacco control organizations built community capacity using 3 processes: developing relationships and partnerships, raising awareness, and creating collective power. CONCLUSION: Contextual, cultural processes, and historical references used by African American tobacco control organizations provide insight into how to engage African American communities in tobacco control efforts and achieve tobacco-related health parity. Public health professionals and nurses should be aware of these and other strategies that may increase the involvement of African American communities in tobacco control.


Assuntos
Negro ou Afro-Americano/estatística & dados numéricos , Competência Cultural , Educação em Saúde/organização & administração , Disparidades nos Níveis de Saúde , Saúde Pública , Abandono do Hábito de Fumar/etnologia , Fumar/etnologia , Conscientização , Comportamento Cooperativo , Educação em Saúde/métodos , Promoção da Saúde/métodos , Humanos , Entrevista Psicológica , Modelos Psicológicos , Fumar/epidemiologia , Fumar/psicologia , Abandono do Hábito de Fumar/métodos , Abandono do Hábito de Fumar/psicologia , Prevenção do Hábito de Fumar , Marketing Social , Gravação em Fita , Estados Unidos/epidemiologia
16.
Ethn Dis ; 30(Suppl 2): 719-734, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33250619

RESUMO

Black/African American populations are underrepresented as participants in dementia research. A major barrier to participation of African American older adults in dementia research is a tendency to distrust research institutions owing to both historical and contemporary racism. Building on the Ford framework, the objective of our study was to examine factors that influence participation in dementia research among African American older adults and caregivers, with an emphasis on understanding factors related to trust. Data were collected during January 2019 and March 2020 from 10 focus groups with African American older adults (n=91), 5 focus groups with caregivers (n=44), and interviews with administrators of community-based organizations (n=11), and meetings with our Community Advisory Board. Inductive/deductive content analysis was used to identify themes. The results identified an overall tension between distrust of researchers and a compelling desire to engage in dementia research. This overarching theme was supported by six themes that provided insights about the multiple layers of distrust, as well as expectations about the appropriate conduct of researchers and academic institutions. Strong commitment to the community was identified as a priority. The findings suggest that a paradigm shift is needed to increase the representation of African Americans in dementia research. In this new paradigm, earning the trust of African American communities becomes a systemic endeavor, with academic, state, and national institutions deeply committed to earning the trust of African American communities and guiding researchers in this endeavor. The findings also generated actionable recommendations to help improve representation of African American older adults in dementia research.


Assuntos
Negro ou Afro-Americano/psicologia , Ensaios Clínicos como Assunto/psicologia , Demência , Seleção de Pacientes , Confiança , Negro ou Afro-Americano/estatística & dados numéricos , Idoso , Beneficência , Feminino , Grupos Focais , Humanos , Relações Interpessoais , Masculino , Pessoa de Meia-Idade
17.
Nurs Outlook ; 56(6): 322-9, 2008.
Artigo em Inglês | MEDLINE | ID: mdl-19041454

RESUMO

People can live longer and healthier lives by engaging in physical activity (PA). The purpose of this article is to assess the social cognitive theory (SCT) in relation to its relevance to produce cultural-specific directions for gerontological nursing practice in order to guide the design of PA interventions for Korean-American elders. SCT is compared to the Korean cultural, social, and health belief system and is analyzed and evaluated based on 3 criteria: assumptions of the theory, completeness and consistency, and essence of nursing. Within the Korean culture, as presumed in the SCT and the nursing paradigm, health-promoting behavior, such as PA, is conceptualized as the desire for a higher level of health rather than a fear of disease as is proposed by other health behavior theories. SCT with the integration of Korean culture recognizes cultural, developmental, societal, and other external constraints that may help in formulating interventions and better understanding of the limits faced by older Korean-Americans (OKAs) in their pursuit of routine PA.


Assuntos
Idoso/psicologia , Asiático/etnologia , Atitude Frente a Saúde/etnologia , Exercício Físico/psicologia , Comportamentos Relacionados com a Saúde/etnologia , Teoria Psicológica , Budismo/psicologia , Confucionismo/psicologia , Competência Cultural , Enfermagem Geriátrica , Conhecimentos, Atitudes e Prática em Saúde , Humanos , Coreia (Geográfico)/etnologia , Papel do Profissional de Enfermagem/psicologia , Filosofias Religiosas/psicologia , Autoimagem , Comportamento Social , Identificação Social , Valores Sociais , Enfermagem Transcultural , Estados Unidos
18.
J Transcult Nurs ; 29(3): 249-257, 2018 05.
Artigo em Inglês | MEDLINE | ID: mdl-28826340

RESUMO

INTRODUCTION: Nearly half of Americans have a chronic health condition related to unhealthful behavior. One in four Americans is an immigrant; yet immigrants' health has been studied little, particularly among Asian American subpopulations. METHODOLOGY: Years lived in United States, hypertension, diabetes, smoking, walking, adiposity, and fruit/vegetable variables in the 2011-2012 California Health Interview Survey were analyzed to examine the influence of nativity on chronic health conditions and health behaviors in 555 adult Filipinos, the second largest Asian American immigrant subpopulation. RESULTS: Recent and long-term immigrant Filipinos had higher odds of having hypertension and diabetes, but lower odds of smoking and overweight/obesity compared with second-generation Filipinos. DISCUSSION: Being born in the United States may be protective against chronic health conditions, but not for healthful behaviors among Filipinos. Chronic disease prevention and health promotion strategies should consider nativity/length of residence, which may be a more consequential health determinant than other immigration and acculturation characteristics.


Assuntos
Aculturação , Asiático/psicologia , Doença Crônica/psicologia , Comportamentos Relacionados com a Saúde/etnologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Asiático/estatística & dados numéricos , California/epidemiologia , California/etnologia , Doença Crônica/epidemiologia , Doença Crônica/etnologia , Estudos Transversais , Emigrantes e Imigrantes/psicologia , Emigrantes e Imigrantes/estatística & dados numéricos , Feminino , Humanos , Renda/estatística & dados numéricos , Masculino , Pessoa de Meia-Idade , Fatores de Risco , Fatores Socioeconômicos
19.
J Assoc Nurses AIDS Care ; 29(5): 737-748, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29773302

RESUMO

In the United States, Black men who have sex with men (MSM), between the ages of 18 and 34 years, have the highest rates of new HIV infections. The prevalence of HIV in this population is three to four times higher than their White MSM counterparts. Twelve Black MSM from the Bay Area, nine with HIV and three without HIV, were interviewed regarding their experiences and perceived risks of acquiring HIV. Narrative analysis revealed these themes: (a) tested regularly for HIV, (b) HIV knowledge varied before arriving in San Francisco, (c) condom use typically nonexistent when under the influence of alcohol and other drugs, (d) inability to negotiate sex and condom usage, and (e) sense of anticipation, resignation, and acceptance about acquiring HIV. Implications of this study highlight the need for Black MSM to have earlier HIV prevention education, including condom negotiation skills, particularly when under the influence of drugs and/or alcohol.


Assuntos
População Negra/psicologia , Infecções por HIV/diagnóstico , Conhecimentos, Atitudes e Prática em Saúde , Homossexualidade Masculina/etnologia , Parceiros Sexuais/psicologia , Transtornos Relacionados ao Uso de Substâncias/complicações , Adolescente , Adulto , Negro ou Afro-Americano/psicologia , Preservativos/estatística & dados numéricos , Infecções por HIV/psicologia , Homossexualidade Masculina/psicologia , Humanos , Entrevistas como Assunto , Masculino , Narração , Percepção , Pesquisa Qualitativa , Assunção de Riscos , São Francisco/epidemiologia , Sexo sem Proteção/estatística & dados numéricos , Adulto Jovem
20.
FASEB J ; 20(3): 509-11, 2006 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-16319133

RESUMO

We have previously identified a novel complex between the platelet-derived growth factor (PDGF)beta receptor and the sphingosine 1-phosphate receptor-1 (S1P1). The complex permits the utilization of active G-protein subunits (made available by constitutively active S1P1 receptor) by the PDGFbeta receptor kinase to transmit signals to p42/p44 MAPK in response to PDGF. Therefore, an inverse agonist of the S1P1 receptor is predicted to reduce signal transduction from PDGFbeta receptor tyrosine kinase by blocking the constitutive activity of the G-protein coupled receptor. SB649146 is a novel inverse agonist of the S1P1 receptor. First, SB649146 displaced the S1P1 receptor agonist dihydrosphingosine 1-phosphate from membranes expressing the recombinant S1P1 receptor. Second, SB649146 reduced basal recombinant S1P1 receptor-induced GTPgammaS binding and S1P-induced GTPgammaS binding in membranes. Third, SB649146 blocked the S1P-induced activation of p42/p44 MAPK in airway smooth muscle cells, a response that is mediated by the S1P1 receptor. We now report that inverse agonism of the S1P1 receptor with SB649146 reduced the endocytosis of the PDGFbeta receptor-S1P1 receptor complex and the stimulation of p42/p44 MAPK and cell migration in response to PDGF. These findings are the first to report that a GPCR inverse-agonist reduces growth factor-induced receptor tyrosine kinase signaling, fundamentally broadening their mechanism of action. The data obtained with SB649146 also suggest that the constitutively active endogenous S1P1 receptor enhances PDGF-induced cell migration.


Assuntos
Movimento Celular/fisiologia , Fator de Crescimento Derivado de Plaquetas/farmacologia , Receptor beta de Fator de Crescimento Derivado de Plaquetas/fisiologia , Receptores de Lisoesfingolipídeo/agonistas , Animais , Linhagem Celular , Movimento Celular/efeitos dos fármacos , Células Cultivadas , Flavonoides/farmacologia , Cobaias , Humanos , Rim , Sistema de Sinalização das MAP Quinases/efeitos dos fármacos , Células PC12 , Ratos , Receptores de Lisoesfingolipídeo/fisiologia , Transfecção , Tirfostinas/farmacologia
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