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1.
PLoS Biol ; 15(6): e2001414, 2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28662064

RESUMO

In many disciplines, data are highly decentralized across thousands of online databases (repositories, registries, and knowledgebases). Wringing value from such databases depends on the discipline of data science and on the humble bricks and mortar that make integration possible; identifiers are a core component of this integration infrastructure. Drawing on our experience and on work by other groups, we outline 10 lessons we have learned about the identifier qualities and best practices that facilitate large-scale data integration. Specifically, we propose actions that identifier practitioners (database providers) should take in the design, provision and reuse of identifiers. We also outline the important considerations for those referencing identifiers in various circumstances, including by authors and data generators. While the importance and relevance of each lesson will vary by context, there is a need for increased awareness about how to avoid and manage common identifier problems, especially those related to persistence and web-accessibility/resolvability. We focus strongly on web-based identifiers in the life sciences; however, the principles are broadly relevant to other disciplines.


Assuntos
Disciplinas das Ciências Biológicas/métodos , Biologia Computacional/métodos , Mineração de Dados/métodos , Design de Software , Software , Disciplinas das Ciências Biológicas/estatística & dados numéricos , Disciplinas das Ciências Biológicas/tendências , Biologia Computacional/tendências , Mineração de Dados/estatística & dados numéricos , Mineração de Dados/tendências , Bases de Dados Factuais/estatística & dados numéricos , Bases de Dados Factuais/tendências , Previsões , Humanos , Internet
2.
Cell Death Dis ; 8(2): e2623, 2017 02 23.
Artigo em Inglês | MEDLINE | ID: mdl-28230857

RESUMO

Neuronal damage induced by injury, stroke, or neurodegenerative disease elicits swift immune responses from glial cells, including altered gene expression, directed migration to injury sites, and glial clearance of damaged neurons through phagocytic engulfment. Collectively, these responses hinder further cellular damage, but the mechanisms that underlie these important protective glial reactions are still unclear. Here, we show that the evolutionarily conserved trimeric protein phosphatase 4 (PP4) serine/threonine phosphatase complex is a novel set of factors required for proper glial responses to nerve injury in the adult Drosophila brain. Glial-specific knockdown of PP4 results in reduced recruitment of glia to severed axons and delayed glial clearance of degenerating axonal debris. We show that PP4 functions downstream of the the glial engulfment receptor Draper to drive glial morphogenesis through the guanine nucleotide exchange factor SOS and the Rho GTPase Rac1, revealing that PP4 molecularly couples Draper to Rac1-mediated cytoskeletal remodeling to ensure glial infiltration of injury sites and timely removal of damaged neurons from the CNS.


Assuntos
Axônios/metabolismo , Proteínas de Drosophila/metabolismo , Drosophila/metabolismo , Neuroglia/metabolismo , Fagócitos/metabolismo , Fosfoproteínas Fosfatases/metabolismo , Animais , Encéfalo/metabolismo , Regulação da Expressão Gênica/fisiologia , Proteínas de Membrana/metabolismo , Neurônios/metabolismo , Fagocitose/fisiologia , Transdução de Sinais/fisiologia
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