RESUMO
We perform Monte Carlo simulations to show that the dual-axes (DA) confocal architecture has superior rejection of multiply scattered photons in tissue to that of single axis. As a result, the DA configuration provides improved signal-to-noise ratio and dynamic range, and thus is sensitive to ballistic photons from deeper within tissue, features that are particularly useful for performing vertical cross-sectional reflectance images in tissue.
Assuntos
Microscopia Confocal/instrumentação , Óptica e Fotônica , Anisotropia , Fenômenos Químicos , Físico-Química , Desenho de Equipamento , Microscopia Confocal/métodos , Modelos Estatísticos , Modelos Teóricos , Método de Monte Carlo , Fótons , Espalhamento de RadiaçãoRESUMO
The first, to our knowledge, miniature dual-axes confocal microscope has been developed, with an outer diameter of 10 mm, for subsurface imaging of biological tissues with 5-7 microm resolution. Depth-resolved en face images are obtained at 30 frames per second, with a field of view of 800 x 100 microm, by employing a two-dimensional scanning microelectromechanical systems mirror. Reflectance and fluorescence images are obtained with a laser source at 785 nm, demonstrating the ability to perform real-time optical biopsy.