RESUMO
The protein profile and antigenic properties of lung-stage larvae of Ascaris lumbricoides and A. suum were studied using 2-dimensional electrophoresis and immunoblot analysis, respectively. The protein profiles of the 2 parasites were identical except for the presence of only 1 major protein spot specific for each. There was a complete cross-reactivity between the 2 parasites at the immunological level, and no specific antigen was recognized using specific antibody raised against the 2 parasites in rabbits.
Assuntos
Antígenos de Helmintos/análise , Ascaris lumbricoides/química , Ascaris suum/química , Proteínas de Helminto/análise , Animais , Ascaris lumbricoides/imunologia , Ascaris suum/imunologia , Reações Cruzadas , Eletroforese em Gel Bidimensional , Feminino , Humanos , Immunoblotting , Larva/química , Larva/imunologia , Pulmão/parasitologia , Especificidade da Espécie , SuínosRESUMO
The study was carried out from July 2007 to June 2008 in Wolaita Sodo Abattoir to assess the status of hydatidosis in cattle. Routine meat inspection, hydatid cyst count and characterization were conducted. Out of 400 cattle slaughtered in Wolaita Sodo Abattoir 64 (16%) animals were found harboring hydatid cysts. Thorough meat inspection in the abattoir revealed that 74 visceral organ were found harboring one or more hydatid cysts. The infection of the lung, liver, spleen and kidney were found to be 45.94% 45.94%, 6.75% and 1.35% respectively. From the total of 283 hydatid cysts counted 153(54.06%), 17(6.00%), 5(1.76%), 108(38.16%) were found to be small, medium, large and calcified cysts respectively and 170(60.28%), 5(1.76%) and 108(38.16%) were sterile, fertile and calcified cysts respectively. The rate of cyst calcification was higher in the liver than in the lung while fertility rate was higher among the cysts of the lung. Hydatid cyst viability rate of 40% was observed.