[Absorbed components analysis of Zhitong Huazheng Capsules in rat serum by UPLC-Q-TOF/MS].

To identify and analyze the constituents in rat serum after oral administration of Zhitong Huazheng capsule (ZTHZC), and provide a reference for its further research on pharmacodynamics material basis. Female Wistar rats were selected as experimental animals, and received intragastric administration of ZTHZC at a dose of 1.5 g·kg⁻¹. After the serum samples were collected, the absorbed prototype components in rat serum were identified and analyzed by using ultra-high performance liquid chromatography-quadrupole-time-of-flight/mass spectrometry (UPLC-Q-TOF/MS) combined with multivariate statistical analysis．The results showed, a total of fifteen absorbed constituents were identified, all of which were prototype components, including Danshensu, salvianolic acid A, B, C, D, 9,12-dihydroxy-15-nonadecanoicacid, linoleic acid, ethyl palmitoleate, tetrahydropalmatine, fumarate A, astragaloside A, astragaloside II, saponin, locustin and luteolin. This experiment showed that these fifteen components absorbed into blood may be the potential bioactive components in ZTHZC, providing a scientific basis for clarifying its material basis in pharmacodynamics.

[Determination of Heavy Metal Baseline Values and Analysis of Its Accumulation Characteristics in Agricultural Land in Chongqing].

In order to understand the accumulation of heavy metals of agricultural land in Chongqing and make clear priority control elements, the soil heavy metal baseline values need to be determined. Based on geochemical baselines, a total of 214 surface soil samples of six soil types were collected according to stratified sampling. Under strict quality control, the As, Cd, Cr, Cu, Hg, Ni, Pb, and Zn content in each sample was measured. The baseline values of eight heavy metals were determined using mathematical statistics, iterative culling, and cumulative frequency curve methods. The accumulation of heavy metals in agricultural soils in Chongqing was evaluated using the geochemical baseline pollution and geological accumulation index methods. The mean baseline values of As, Cd, Cr, Cu, Hg, Ni, Pb, and Zn in agricultural land in Chongqing were 5.83, 0.25, 66.78, 25.45, 0.069, 29.90, 26.18, and 78.44 mg·kg-1, respectively, which agreed with the historical findings. Evaluation of the geochemical baseline pollution index method showed that the accumulation of As, Hg, and Zn in soil was the most prominent, with 14.65%, 11.82%, and 3.88% of the samples reaching severe accumulation, respectively. The proportions of moderate accumulation of As, Cd, Cr, Cu, Hg, Ni, Pb, and Zn were 12.12%, 6.50%, 3.79%, 6.50%, 15.27%, 5.29%, 5.45%, and 17.96%, respectively, of which As, Hg, and Zn were relatively large. The geological accumulation index indicated that more than 93% of the samples of Cd, Cr, Cu, Ni, and Pb were in a state of no accumulation. The proportions of mild accumulation of Hg, Zn, and As were 26.60%, 21.84%, and 21.21%, respectively. Only 5.56% and 0.49% of the samples had moderate accumulation of As and Zn. Different soil types have different properties. All soil types in the sample had different degrees of accumulation of the eight kinds of heavy metals. The accumulation of heavy metals in paddy and purple soils was lower than that in other soil types. For the soils of different geological and geomorphic types, the Ki values of As in the limestone middle and low mountain area of Daba Mountain in Northeast Chongqing and the low mountain area of the limestone of Wuling Mountain in Southeast Chongqing were relatively large; the rest were not significantly different, and all belonged to the non-accumulation and mild accumulation ranges. Both evaluation methods showed that the main accumulated heavy metals in agricultural land soil in Chongqing was As, followed by Hg and Zn, and management and prevention of the accumulation of these elements should be strengthened.

MicroRNA-139-5p inhibits cell proliferation and invasion by targeting insulin-like growth factor 1 receptor in human non-small cell lung cancer.

INTRODUCTION: Increasing evidence suggested that microRNAs (miRNAs) play a critical role in tumorigenesis. Decreased expression of miRNA-139-5p has been observed in various types of cancers. However, the biological function of miRNA-139-5p in non-small cell lung cancer (NSCLC) is still largely unknown. METHODS: Quantitative real-time PCR (qRT-PCR) was used to explore the expression level of miRNA-139-5p in NSCLC tissues and cell lines. Then, we investigated the role of miRNA-139-5p to determine its potential roles on lung cancer cell proliferation, migration and invasion in vitro. A luciferase reporter assay was performed to confirm the target gene of miRNA-139-5p and the results were validated in renal cancer cells. RESULTS: miRNA-139-5p was significantly decreased in NSCLC tissues and cell lines. Over-expression of miRNA-139-5p could inhibit lung cancer cell proliferation, migration, and invasion in vitro. Furthermore, we identified insulin-like growth factor 1 receptor (IGF1R) as a target of miR-139-5p and miR-139-5p function as a tumor suppressor via targeting IGF1R in NSCLC. CONCLUSIONS: Our results indicated that miR-139-5p acts as a tumor suppressor in NSCLC partially via down-regulating IGF1R expression.