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We present a comprehensive theoretical and experimental investigation of the hydrogenation and dehydrogenation of dibenzyltoluene (DBT) using Pd-, Pt-, Ru-, and Rh-supported metal catalysts to identify the optimal catalysts for hydrogen storage and release processes. Our results demonstrated significant variation in the catalytic activity of the metal catalysts. 5 wt % Rh/Al2O3 and 5 wt % Pt/Al2O3 showed the highest activity for hydrogenation and dehydrogenation with the highest selectivity and turnover frequency (TOF), respectively. Conversely, 5 wt % Pd/Al2O3 and 5 wt % Ru/Al2O3 exhibited lower catalytic activity toward full hydrogenation and dehydrogenation. Rh/Al2O3 showed the best catalytic hydrogenation activity with a TOF of 26.49 h-1 and a hydrogenation degree of 92.69% in 2 h, while Pt/Al2O3 exhibited the best catalytic dehydrogenation activity with a released H2 volume of 3755 mL, a dehydrogenation degree of 78.23%, and a TOF of 39.56 h-1 in 2 h. Additionally, we estimated the activation energies for hydrogenation and dehydrogenation to be 67.20 and 82.78 kJ/mol, respectively. Notably, the produced hydrogen gas was of high purity and suitable for use in fuel cells. Density functional theory (DFT) calculations were used to analyze the adsorption structure and reaction energy changes of all intermediate products of DBT on the surface of the chosen catalysts. Our research provides valuable insights into developing efficient catalysts for liquid organic hydrogen carriers.
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BACKGROUND: The bitter taste receptor gene TAS2R38 is a member of the human TAS2R gene family. Polymorphisms in TAS2R38 affect the ability to taste the bitterness of phenylthiourea (PTC) compounds, thus affecting an individual's food preference and health status. METHODS: We investigated polymorphisms in the TAS2R38 gene and the sensitivity to PTC bitterness among healthy Chinese college students in Hubei province. The association of TAS2R38 polymorphisms and PTC sensitivity with body mass index (BMI), food preference, and health status was also analyzed. A total of 320 healthy college students were enrolled (male: 133, female: 187; aged 18-23 years). The threshold value method was used to measure the perception of PTC bitterness, and a questionnaire was used to analyze dietary preferences and health status. Polymerase chain reaction (PCR) was used to analyze polymorphisms at three common TAS2R38 loci (rs713598, rs1726866, and rs10246939). RESULTS: In our study population, 65.00% of individuals had medium sensitivity to the bitterness of PTC; in contrast, 20.94% were highly sensitive to PTC bitterness, and 14.06% were not sensitive. For the TAS2R38 gene, the PAV/PAV and PAV/AAI diplotypes were the most common (42.19% and 40.63%, respectively), followed by the homozygous AVI/AVI (8.75%) and PAV/AVI (5.00%) diplotypes. CONCLUSION: There was a significant correlation between the sensitivity to PTC bitterness and sex, but there was no correlation between the common diplotypes of TAS2R38 and gender. Polymorphisms in the TAS2R38 gene were associated with the preference for tea, but not with one's native place, BMI, health status, or other dietary preferences. There was no significant correlation between the perception of PTC bitterness and one's native place, BMI, dietary preference, or health status. We hope to find out the relationship between PTC sensitivity and TAS2R38 gene polymorphisms and dietary preference and health status of Chinese population through this study, providing relevant guidance and suggestions for dietary guidance and prevention of some chronic diseases in Chinese population.
Assuntos
Feniltioureia , Receptores Acoplados a Proteínas G , Paladar , Feminino , Humanos , Masculino , Povo Asiático/genética , Receptores Acoplados a Proteínas G/genética , Estudantes , Paladar/genéticaRESUMO
Epithelial-mesenchymal transition (EMT), the epithelial cells transdifferentiation into the mesenchymal cells, has been involved in cancer metastasis. Nannocystin ax (NAN) is a cyclodepsipeptide initially isolated from Myxobacterial genus, Nannocystis sp. with anticancer activities. This study was designed to explore the effect of NAN on TGF-ß1-induced EMT in lung cancer cells. The morphological alteration was observed with a microscope. Western blotting and immunofluorescence assays were used to detect the protein expression and the localization. The adhesion and migration were evaluated by adhesion assay and wound healing assay. The mRNA expression of TGF-ß receptor type I (TßRI) was determined by real-time PCR. NAN significantly restrained TGF-ß1-induced EMT morphological changes, the protein expression of E-cadherin, N-cadherin, and Vimentin, etc. TGF-ß1 activated phosphorylation and nuclear translocation of Smad2/3 were inhibited by NAN. Furthermore, NAN suppressed adhesion and migration triggered by TGF-ß1. In addition, NAN significantly down-regulated TßRI on the transcriptional level directly. In summary, these results showed that NAN restrained TGF-ß1-induced epithelial-mesenchymal transition, migration, and adhesion in human lung cancer cells. The underlying mechanism involved the inhibition of Smad2/3 and the TßRI signaling pathway. This study reveals the new anticancer effect and mechanism of NAN.
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Antineoplásicos/farmacologia , Carcinoma Pulmonar de Células não Pequenas/tratamento farmacológico , Adesão Celular/efeitos dos fármacos , Movimento Celular/efeitos dos fármacos , Depsipeptídeos/farmacologia , Transição Epitelial-Mesenquimal/efeitos dos fármacos , Neoplasias Pulmonares/tratamento farmacológico , Compostos Macrocíclicos/farmacologia , Myxococcales/química , Fator 1 de Elongação de Peptídeos/antagonistas & inibidores , Células A549 , Antineoplásicos/isolamento & purificação , Carcinoma Pulmonar de Células não Pequenas/genética , Carcinoma Pulmonar de Células não Pequenas/metabolismo , Carcinoma Pulmonar de Células não Pequenas/patologia , Depsipeptídeos/isolamento & purificação , Humanos , Neoplasias Pulmonares/genética , Neoplasias Pulmonares/metabolismo , Neoplasias Pulmonares/patologia , Compostos Macrocíclicos/isolamento & purificação , Fator 1 de Elongação de Peptídeos/metabolismo , Fosforilação , Receptor do Fator de Crescimento Transformador beta Tipo I/genética , Receptor do Fator de Crescimento Transformador beta Tipo I/metabolismo , Transdução de Sinais , Proteína Smad2/metabolismo , Proteína Smad3/metabolismoRESUMO
Colorectal cancer (CRC) is one of the most common causes of cancer-related death worldwide. Nannocystin ax (NAN), a 21-membered cyclodepsipeptide initially isolated from myxobacteria of the Nannocystis genus, was found to target the eukaryotic elongation factor 1A (eEF1A). The current study was designed to evaluate the anticancer effect and underlying mechanisms of NAN with in vitro and in vivo models. Results showed that NAN induced G1 phase cell cycle arrest and caspase-independent apoptosis in HCT116 and HT29 human CRC cells. NAN significantly downregulated cyclin D1 level in a short time, but NAN did not affect the transcription level and ubiquitin-dependent degradation of cyclin D1. Furthermore, NAN treatment directly targeted eEF1A and partially decreased the synthesis of new proteins, contributing to the downregulation of cyclin D1. Besides, NAN significantly suppressed tumor growth in the zebrafish xenograft model. In conclusion, NAN triggered G1 phase cell cycle arrest through cyclin D1 downregulation and eEF1A-targeted translation inhibition and promoted caspase-independent apoptosis in CRC cells.
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Antineoplásicos/uso terapêutico , Neoplasias do Colo/tratamento farmacológico , Depsipeptídeos/uso terapêutico , Animais , Antineoplásicos/farmacologia , Apoptose/efeitos dos fármacos , Caspases/metabolismo , Neoplasias do Colo/genética , Neoplasias do Colo/metabolismo , Ciclina D1/genética , Ciclina D1/metabolismo , Depsipeptídeos/farmacologia , Regulação para Baixo/efeitos dos fármacos , Pontos de Checagem da Fase G1 do Ciclo Celular/efeitos dos fármacos , Células HCT116 , Células HT29 , Humanos , Potencial da Membrana Mitocondrial/efeitos dos fármacos , Fator 1 de Elongação de Peptídeos/genética , Peixe-ZebraRESUMO
A synthetic study on the construction of the tetracyclic core structure of dysiherbols A-C is presented herein. In this synthesis, intramolecular [2 + 2] cycloaddition introduces a fused 6/4 ring system, followed by a Pd-catalyzed semipinacol rearrangement/Csp2-H arylation cascade to construct the ring C, and visible-light-mediated ring-opening of cyclopropyl silyl ether installs the tetracyclic core of dysiherbols.
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Circular RNAs (circRNAs), a novel type of endogenous RNAs, have become a subject of intensive research. It has been found that circRNAs are important players in cell differentiation and tissue homeostasis, as well as disease development. Moreover, the expression of circRNAs is usually not correlated with their parental gene expression, indicating that they are not only a steady-state by-product of mRNA splicing but a product of variable splicing under novel regulation. Sequence conservation analysis has also demonstrated that circRNAs have important non-coding functions. CircRNAs exist as a covalently closed loop form in mammalian cells, where they regulate cellular transcription and translation processes. CircRNAs are built from pre-messenger RNAs, and their biogenesis involves back-splicing, which is catalyzed by spliceosomes. The splicing reaction gives rise to three different types of intronic, exotic and exon-intron circular RNAs. Due to higher nuclease stability and longer half lives in cells, circRNAs are more stable than linear RNAs and have enormous clinical advantage for use as diagnostic and therapeutic biomarkers for disease. In recent years, it has been reported that circRNAs in stem cells play a crucial role in stem cell function. In this article, we reviewed the general feature of circRNAs and the distinct roles of circRNAs in stem cell biology, including regulation of stem cell self-renewal and differentiation. CircRNAs have shown unique expression profiles during differentiation of stem cells and could serve as promising biomarkers of these cells. As circRNAs play pivotal roles in stem cell regulation as well as the development and progression of various diseases, we also discuss opportunities and challenges of circRNA-based treatment strategies in future effective therapies for promising clinical applications.