RESUMO
The study investigated the effect of recombinant human osteogenic protein-1 (rhOP-1) expressed in prokaryocyte, to promote the healing of alveolar socket. A model of rabbit extracted socket into which the composites of rhOP-1 and gelatin sponge was immediately implanted was created and the osteoinduction of rhOP-1 was assessed by histological method, quantitative measurement of calcium content and alkaline phosphatase (ALP) activity. The result of histology showed that bone healing in rhOP-1 side is 4-6 weeks earlier than that of the control side. ALP activity and calcium content in rhOP-1 side were significantly high compared with that of the control side. rhOP-1 has a satisfactory osteoinduction ability to promote the healing of extracted socket.
Assuntos
Proteínas Morfogenéticas Ósseas/metabolismo , Regeneração Óssea/fisiologia , Consolidação da Fratura , Fraturas Ósseas , Proteínas Recombinantes/metabolismo , Alvéolo Dental , Fosfatase Alcalina/metabolismo , Animais , Proteína Morfogenética Óssea 7 , Proteínas Morfogenéticas Ósseas/genética , Cálcio/metabolismo , Humanos , Traumatismos Mandibulares/patologia , Osteogênese/fisiologia , Coelhos , Distribuição Aleatória , Proteínas Recombinantes/genética , Alvéolo Dental/citologia , Alvéolo Dental/patologia , Alvéolo Dental/fisiologiaRESUMO
PURPOSE: To explore the correlation between gingipain K (Kgp) and inflammatory reaction of gum in the process of orthodontic treatment, and analyse the role of Kgp in the development of gingivitis during orthodontic treatment. METHODS: Totally 45 orthodontic healthy teenagers were randomly chosen for the study. The subgingival plaques were collected simultaneously before orthodontic treatment and 3 months after treatment. 16S rDNA PCR technique was used to detect Porphyromonas gingivalis (P.g) and Kgp. SPSS17.0 software package was used for statistical analysis. RESULTS: The detection rate of Kgp was 35.71% before orthodontic treatment and 67.86% after treatment. Positive correlation (P<0.05) was observed between Kgp and gingival inflammation. CONCLUSIONS: Fixed orthodontic appliances cause plaque accumulation, accordingly slight gingiva inflammation and the increament of P.gingivalis in the early stage.
Assuntos
Adesinas Bacterianas , Cisteína Endopeptidases , Placa Dentária , Gengivite , Aparelhos Ortodônticos , Porphyromonas gingivalis , Adolescente , Cisteína Endopeptidases Gingipaínas , Gengiva , Líquido do Sulco Gengival , HumanosRESUMO
OBJECTIVE: To investigate new bone formation of alveolar augmentation with recombinant human bone morphogenetic protein-7 (rhBMP-7) expressed in prokaryocyte. METHODS: To create the model of rabbit extraction socket into which the composites of rhBMP-7 and the gelatin sponge was immediately implanted, then the samples were investigated 2, 4, 8 and 12 weeks postoperatively by gross observation, scanning electron microscope (SEM), quantitative measurement of calcium content and alkaline phosphatase (ALP) activity. RESULTS: There was significant difference in height of alveolar ridge absorpted between the experimental groups and the carrier control groups through gross observation. The result of SEM showed that bone healing in rhBMP-7 groups was 4-6 weeks earlier than that of control groups. ALP activity in rhBMP-7 groups were obviously high compared with that of control groups. CONCLUSION: The BMP-7 has a satisfactory osteoinduction ability to promote new bone formation and prevent alveolar bone absorption.