Numerical Simulation and Experimental Study Regarding the Cross-Sectional Morphology of PEEK Monofilament Deposition During FDM-Based 3D Printing.

Polyether-ether-ketone (PEEK) is widely used in the field of biomedical engineering because of its excellent mechanical properties, chemical stability, and biocompatibility. Fused deposition modeling (FDM), which is a typical 3D printing process, can achieve low-cost and high-efficiency printing of complex PEEK structures. However, poor monofilament deposition quality leads to rough surfaces on macroscopic printed parts, low dimensional accuracy, and weak interlayer bonding, which are urgent problems to be solved. In this study, considering the shear thinning characteristic of PEEK, a numerical model for monofilament deposition was constructed by using the finite volume method. This model revealed the influences of process parameters on the monofilament cross-sectional profiles and achieved predictions of monofilament cross-sectional profiles during FDM-based 3D printing of PEEK. The average relative error of the monofilament cross-sectional area predictions was 7.68%. The average relative error of the monofilament cross-sectional aspect ratio predictions was 12.06%. It was also found that there are three typical deposited monofilament cross-sectional profile shapes, i.e., a capsule shape, a bread shape, and a circular shape. These three shapes occurred because of the combined effect of the layer thickness and the extrusion width during the extrusion and deposition of PEEK. These revealed monofilament cross-sectional profiles provide the basis for accurate nozzle motion trajectory planning, and they lay a foundation for surface roughness predictions and dimensional accuracy control during the FDM-based 3D printing of PEEK.

Three-Dimensional Morphology and Contour Quality of Conductive Lines Printed by High-Viscosity Paste Jetting.

The utilization of high-viscosity paste jetting technology has the potential to significantly expand the range of available materials and enhance the three-dimensional forming efficiency compared to inkjet printing. In this study, the three-dimensional morphology and contour quality of lines printed using high-viscosity silver paste were investigated. Four types of lines were identified based on differences in the printing shape, and contour fluctuation evaluation indices were defined in both the transverse and longitudinal directions to establish quantitative distinction principles. Based on the research of inkjet printing, a modified theoretical model relating the dimensionless line width and droplet spacing was established considering the cross-sectional characteristics of the lines printed by high-viscosity paste jetting. With the establishment of mathematical models, distinction criteria between various line shapes were obtained and the printable range of uniform lines was determined. Finally, based on response surface methodology, the influences of single droplet jetting parameters, the line printing speed, and their interactions on the line contour fluctuation were analyzed. This study involved theoretical and experimental research on the line morphology and contour quality, which can provide support for control of the line printing quality in high-viscosity paste jetting technology.

Functional orthologs of honeybee CYP6AQ1 in stingless bees degrade the butenolide insecticide flupyradifurone.

Flupyradifurone (FPF), a novel butenolide insecticide binding to nicotinic acetylcholine receptors (nAChRs), has been shown to be less acutely toxic to western honey bees (Apis mellifera) than other insecticides such as neonicotinoids sharing the same target-site. A previous study revealed that this is due to enhanced oxidative metabolism of FPF, mediated by three cytochrome P450 monooxygenases (P450s), including CYP6AQ1. Therefore, we followed a toxicogenomics approach and investigated the potential role of functional CYP6AQ1 orthologs in FPF metabolism from eight different bee species, including stingless bees (Tribe: Meliponini). We conducted a phylogenetic analysis on four stingless bee species, including Frieseomelitta varia, Heterotrigona itama, Melipona quadrifasciata and Tetragonula carbonaria to identify CYP6AQ1-like functional orthologs. Three non-Meliponini, but tropical bee species, i.e., Ammobates syriacus, Euglossa dilemma and Megalopta genalis were analyzed as well. We identified candidate P450s in all (neo)tropical species with greater than 61% and 67% predicted protein sequence identities when compared to A. mellifera CYP6AQ1 and Bombus terrestris CYP6AQ26, respectively. Heterologous expression in High Five insect cells of these functional orthologs revealed a common coumarin substrate profile and a preference for the O-debenzylation of bulkier substrates. Competition assays using the fluorescent probe substrate 7-benzyloxymethoxy-4-trifluoromethylcoumarin (BOMFC) with these enzymes indicated inhibition of BOMFC metabolism by increasing concentrations of FPF. Furthermore, UPLC-MS/MS analysis revealed the capacity of all CYP6AQ1-like orthologs to metabolize FPF by hydroxylation in vitro at various levels, indicating a conserved FPF detoxification potential in different (neo)tropical bee species including Meliponini. This research, employing a toxicogenomics approach, provides important insights into the potential of stingless and other tropical bee species to detoxify FPF, and highlights the significance of investigating the detoxification mechanisms of insecticides in non-Apis bee species by molecular tools to inform risk assessment and conservation efforts.

Advances in 3D Printed Electronics: Materials, Processes, Properties and Applications.

The current Special Issue entitled "Advances in 3D printed electronics: materials, processes, properties and applications" aims to discuss the latest developments in the field of the AM of structures or components with reinforcements [...].

Experimental Study of the Jetting Behavior of High-Viscosity Nanosilver Inks in Inkjet-Based 3D Printing.

Inkjet printing of high-viscosity (up to 105 mPa·s) nanosilver inks is an interesting emerging technology to achieve the 3D fully printed fabrication of electronic products. The highly viscous force of the ink makes it impossible to achieve droplet ejection with the traditional piezoelectric-driven drop-on-demand inkjet method. In this study, a pneumatic needle jetting valve is adopted to provide sufficient driving force. A large number of high-viscosity inkjet printing tests are carried out, and the jetting behavior is recorded with a high-speed camera. Different jetting states are determined according to the recorded images, and the causes of their formation are revealed. Additionally, the effects of the operating pressure, preload angle, and fluid pressure on jetting states are elucidated. Furthermore, the jetting phase diagram is obtained with the characterization of the Reynolds number and the printable region is clarified. This provides a better understanding of high-viscosity inkjet printing and will promote the application of high-viscosity inkjet printing in 3D fully printed electronic products.

Simulation and Experimental Study of the Multisized Silver Nanoparticles Sintering Process Based on Molecular Dynamics.

Multisized nanoparticles (MPs) are widely employed as electronic materials to form conductive patterns, benefitting from their excellent sintering properties and mechanical reliability. However, due to the lack of effective detection methods for the real-time sintering process, it is difficult to reveal the sintering behavior during the MPs sintering process. In this work, a molecular dynamics method is used to track the trajectory of silver atoms. The melting behavior of a single nanoparticle (SP) is first discussed. The structural evolution of equally sized nanoparticles (EPs) and unequally sized nanoparticles (UPs) during the sintering process is analyzed alongside morphology changes. It is proposed that the UPs sintering process benefits from the wetting behavior of small-sized nanoparticles on the surface of large-sized nanoparticles, and the sintering angle (θ) is proposed as an index to estimate the sintering result of UPs. Based on the works above, three basic sintering modes and one advanced sintering mode in the MP sintering process are analyzed emphatically in this paper, and the roles of different-sized nanoparticles in MPs are concluded from simulation and experimental results. This work provides theoretical support for conductive ink composition design and sintering process optimization.

Prediction of the Surface Roughness in Ultrasonic Vibration-Assisted Grinding of Dental Zirconia Ceramics Based on a Single-Diamond Grit Model.

Ultrasonic vibration-assisted grinding (UVAG) is regarded as a superior method for the fabrication of ceramic dentures, due to its outstanding performance in hard and brittle materials' machining. The surface roughness of dentures has a critical effect on the bonding and wear performance between dentures and natural teeth. Accomplishing the prediction of surface roughness will promote the application of UVAG in dental restoration significantly. However, the investigation about surface roughness modeling in the UVAG of ceramics is limited. In this study, a comprehensive surface roughness model was proposed with the consideration of the diamond grits' random distribution, brittle fracture removal, and ultrasonic vibration characteristics. Based on the indentation fracture removal mechanism, the material removal process was modeled. Rayleigh's probability density function was introduced to characterize the random distribution of the grits. Besides, the ultrasonic vibration was considered via the analysis of the single-diamond grit motion. Finally, the comprehensive model was developed with the consideration of all the diamond grits. Afterward, the verification experiments were carried out. The experimental results agreed well with the model predictions. Therefore, the comprehensive model can be applied to evaluate the surface roughness and can provide an in-depth understanding of the surface formation in the UVAG of ceramics.