Antitumor and Antioxidant Properties of Water-Soluble Polysaccharides from Submerged Mycelium of Flammulina velutipes.

The aim of the study was to evaluate antitumor and antioxidant properties of water-soluble polysaccharides from submerged myceliumn of Flammulina velutipes grown under optimized conditions. The optimization of the nutrient medium composition allowed to increase the biomass yield by more than 2 times (up to 35 g/l) and to reduce the time of the cultivation process. The submerged mycelium of F.velutipes strain Fv-1 contained 14.8% of a water-soluble polysaccharides, 31.6% of proteins, 2.5% of total lipids, vitamins B (B1, B5, B6). The polysaccharides contained glucose, galactose, fucose, mannose, xylose, rhamnose. The proteins contained all the essential amino acids except for tryptophan. Dry powder of the submerged mycelium, water extract of the mycelium and total fraction of the water-soluble polysaccharides demonstrated the antitumor activity against murine lymphocytic leukemia P 388 in vivo. The antitumor activity of the substances was mainly due to the polysaccharides, since their purification increased the tumor growth inhibition. The maximum tumor growth inhibition by the water-soluble polysaccharides amounted to 94%. The total fraction of the water-soluble polysaccharides from F.velutipes strain Fv-1 demonstrated antioxidant activity. The antioxidant capacity (AOC) of the water-soluble fraction from F.velutipes was higher than that of the water-soluble polysaccharides from the sub- merged mycelium of Grifolafrondosa, but inferior to the AOC of the water-soluble polysaccharides from the submerged mycelium of Ganoderma luciduma.

[Antitumor Activity of Polysaccharides from Ganoderma lucidum Mycelium: in vivo Comparative Study].

Fractions of water soluble and alkali soluble polysaccharides, as well as fucogalactan, a water soluble polysaccharide, and xylomannan, an alkali soluble polysaccharide, were isolated from the Ganoderma lucidum submerged mycelium. When administered orally, the polysaccharides showed antitumor activity in vivo on murine models of solid tumors. Xylomannan and fucogalactan showed the highest antitumor activity. Sensitivity to xylomannan was more pronounced in adenocarcinoma Ca755 as compared to the T-cell lymphocytic leukemia P388. The antitumor activity of the water soluble polysaccharides total fractions from the mycelium and fruiting bodies of the G. lucidum strain was almost identical. The maximum antitumor effect of the mycelium water soluble polysaccharides total fraction was observed with the use of the daily dose of 2 mg/kg.