RESUMO
The present work deals with the simultaneous ultrastructure and triple immunofluorescence study of the three main hepatic fibrogenic cells, hepatic stellate cell, myofibroblast (MF), and fibroblast, in a group of hepatitis C virus (HCV) RNA positive patients, as their exact interrelation behavior in vivo with the progress of hepatic fibrosis is still inadequate. In this study, for the first time, cells having the morphological characteristic of MF and not bone marrow fibrocytes were revealed in liver portal vessels. This necessitates the reevaluation of the available knowledge concerning bone marrow fibrocyte. Also, the distribution, cellular interrelations, and the fate of MF were highlighted.
Assuntos
Fibroblastos/ultraestrutura , Células Estreladas do Fígado/ultraestrutura , Hepatite C/patologia , Cirrose Hepática/patologia , Miofibroblastos/ultraestrutura , Ensaio de Imunoadsorção Enzimática , Imunofluorescência , Hepatite C/complicações , Humanos , Ácido Hialurônico , Cirrose Hepática/virologia , Microscopia Confocal , Microscopia Eletrônica de TransmissãoRESUMO
An ultrastructural quantitative assessment of hepatic stellate cells (HSCs) was made in relation to hepatic fibrosis, apoptotic cellular changes, intracellular fat deposition, circulating inflammatory cells in the sinusoids, and the necroinflammatory activity in liver specimens of 33 patients proven to be positive for hepatitis C virus (HCV)-RNA by polymerase chain reaction with the intention that electron microscopy may throw more light on the role of HSCs in the complicated process of fibrogenesis. A detailed review concerning these parameters and observed evidence suggesting the potential properties of HSCs to recycle cellular debris into collagen fibers are reported.
Assuntos
Células Estreladas do Fígado/ultraestrutura , Hepatite C Crônica/patologia , Cirrose Hepática/patologia , Microscopia Eletrônica de Transmissão/métodos , Apoptose/fisiologia , Hepacivirus/genética , Hepacivirus/imunologia , Hepacivirus/isolamento & purificação , Células Estreladas do Fígado/química , Hepatite C Crônica/metabolismo , Humanos , Lipídeos/análise , Cirrose Hepática/metabolismo , RNA Viral/análiseRESUMO
Proper handling and processing of urine sample can greatly improve diagnostic sensitivity. This work investigates the value of agarose cell block technique in processing urine samples simultaneously for light and electron microscopic examination, with the prospect to enhance the quality of diagnosis. The material of this study consisted of 45 voided urine samples, processed for the performance of Papanicolaou-stained urine smears, agarose cell blocks paraffin sections stained with hematoxylin & eosin, and electron microscopy-contrasted ultrathin sections. The studied technique increases the sensitivity of urine cytology and opens a new prospect for cytomorphological study.