RESUMO
The present study determines whether antler extract (AE) possesses inhibitory effects in a prostate cancer (PC) xenograft model and explores the underlying mechanism. After therapeutic intervention for two weeks, AE significantly inhibited prostate cancer xenograft tumor growth by 65.08%, and prostate-specific antigen (PSA) and serum dihydrotestosterone (DHT) levels. However, AE increased the serum testosterone level compared to the vehicle control group. Furthermore, our investigation of the inhibitory effects on angiogenesis and epithelial-to-mesenchymal transition (EMT)-related genes revealed that AE downregulated matrix metalloproteinase 2 (MMP)-2, (MMP)-9, vascular endothelial growth factor (VEGF), zinc finger protein (SNAIL1), twist-related protein 1 (TWIST1), and zinc-finger E-box-binding homeobox 1 (ZEB1) in vivo. In contrast, AE increased tissue inhibitor of MMP (TIMP)-1, (TIMP)-2, and E-cadherin. The results suggest that AE possesses potent anti-PC activity, and this is the first report on the anti-PC effect of AE in vivo.
Assuntos
Chifres de Veado/química , Xenoenxertos , Neoplasias da Próstata/terapia , Animais , Cervos , Di-Hidrotestosterona/sangue , Regulação para Baixo , Transição Epitelial-Mesenquimal/genética , Perfilação da Expressão Gênica , Humanos , Calicreínas/sangue , Masculino , Camundongos Endogâmicos BALB C , Camundongos Nus , Modelos Biológicos , Proteínas de Neoplasias/metabolismo , Neovascularização Patológica , Antígeno Prostático Específico/sangue , Neoplasias da Próstata/imunologia , Neoplasias da Próstata/metabolismo , Neoplasias da Próstata/patologia , Testosterona/sangueRESUMO
In nearshore waters, spatial and temporal scales of waves, tidal currents, and circulation patterns vary greatly. It is, therefore, difficult to combine these factors' effects when trying to predict sediment transport processes. This paper proposes the concept of significant wave velocity, which combines the effects of waves, tides, and ocean currents using the horizontal kinetic energy superposition principle. Through a comparison of the relationship between shear stress at the water-sediment interface and sediment-carrying capacity, assuming equilibrium sediment flux, a new formula for sediment-carrying capacity, which incorporates the concept of significant wave velocities, is derived. Sediment-carrying capacity is a function of the critical velocity, which increases with water depth and decreases with increasing relative roughness of the sea bed. Finally, data from field observation stations and simulations are used to test the proposed formula. The results show that the new formula is in good agreement with both field and simulation data. This new formula for sediment-carrying capacity can be used to simulate nearshore sediment transport.
Assuntos
Sedimentos Geológicos/química , Movimentos da Água , Água/química , Ondas de MaréRESUMO
To investigate the sediment quality of Fudu Estuary waters in East Liaodong Bay, the grain size, concentrations of total organic carbon (TOC) and heavy metal (Cr, Cu, Zn, As, Cd, Pb) of 25 surface sediments were tested. The concentration of heavy metals was found to be higher in the Southwest of Yingkou Port and outside the Fudu Estuary. Cd is the principal contaminant with mild pollutant and moderate ecological risk. Cr may cause negative biological effects. The abnormal enrichment of Cu and TOC may respectively be caused by riverside industrial activities and mariculture. The abnormal enrichment of Pb in Moon Bay Beach and the Lagoon was suggested to be caused by the mooring of boats or excessive tourist activities. Grain size dominates the concentrations of heavy metals in the study area. The impact of coastal engineering on sediment quality through changing sedimentary dynamics should also be given particular attention.