Anthropogenic particle abundance and characteristics in seawater and intertidal sediments of the Tonkin Bay Coast (North Vietnam).

Microplastics (MPs, plastic items from 1 µm to 5 mm in size) are present in all environmental compartments. The evaluation of their concentration, fate, and spatial distribution is still a challenge for the scientific community. This concern is just debuting in developing countries, (i.e., Asia, South America, and Africa). This study deals with the MP contamination in the abiotic marine compartments of Northern Vietnam: seawater and intertidal sediments. Four sites located in the intertidal zone or near the coastline in Tonkin Bay, Vietnam were studied. A total of 16 samples (eight for each compartment) were collected in July 2020 (rainy season) and January 2021 (dry season). Anthropogenic particles (total observed fibers and fragments) were found at levels ranging from 3 to 303 particles/m3 in seawater and from 63 to 955 particles/kg dry weight in sediments. Most of these were fibers less than 300-µm long. Higher levels of seawater at the Nam Dinh site were found in the rainy season compared to the dry one. As the river flow was estimated six times higher during the rainy season than during the dry season, these results suggest the river discharge is a potential source of contamination for the coastal zone. The temporal variability was lower for the sediments than for the seawater, suggesting the long-term integration of the anthropogenic particles in this compartment. A small portion of sorted particles were analyzed by µFTIR (8.35%), and this sub-sample was only composed of fragments. Still, fragments were mostly composed of polypropylene (PP, 82%), polyethylene (PE, 9%), and polystyrene (PS, 9%). The fragment size was similar in the two studied compartments, but it was dependent on polymer types since PS fragments (140 ± 17 µm) were smaller than those made of PE (622 ± 123 µm) and PP (869 ± 905 µm). Future works should investigate the smallest fraction of MP (even nanoplastics) as well as find solutions in order to mitigate MP contamination in the marine environment.

Pentadecaibins I-V: 15-Residue Peptaibols Produced by a Marine-Derived Trichoderma sp. of the Harzianum Clade.

In the course of investigations on peptaibol chemodiversity from marine-derived Trichoderma spp., five new 15-residue peptaibols named pentadecaibins I-V (1-5) were isolated from the solid culture of the strain Trichoderma sp. MMS1255 belonging to the T. harzianum species complex. Phylogenetic analyses allowed precise positioning of the strain close to T. lentiforme lineage inside the Harzianum clade. Peptaibol sequences were elucidated on the basis of their MS/MS fragmentation and extensive 2D NMR experiments. Amino acid configurations were determined by Marfey's analyses. The pentadecaibins are based on the sequences Ac-Aib1-Gly2-Ala3-Leu4-Aib/Iva5-Gln6-Aib/Iva7-Val/Leu8-Aib9-Ala10-Aib11-Aib12-Aib13-Gln14-Pheol15. Characteristic of the pentadecaibin sequences is the lack of the Aib-Pro motif commonly present in peptaibols produced by Trichoderma spp. Genome sequencing of Trichoderma sp. MMS1255 allowed the detection of a 15-module NRPS-encoding gene closely associated with pentadecaibin biosynthesis. Pentadecaibins were assessed for their potential antiproliferative and antimicrobial activities.

Trophic dilution of rare earth elements along the food chain of the Seine estuary (France).

Society's interest in rare earth elements (REEs) and their increasing use in many fields is leading to enrichments in aquatic environments, such as estuaries. This study of the Seine estuary assessed the distribution of REEs along the food web, including different species from 5 phyla representing different trophic levels. Total REE concentrations, which were higher in algae, mollusks, crustaceans and annelids (4.85-156; 1.59-4.08; 2.48 ± 1.80 and 0.14 ± 0.11 µg/g dw, respectively) than in vertebrates (0.03-0.15 µg/g dw), correlated with δ15N indicated a trophic dilution. REE contributions in the studied species were higher for light REEs than for heavy and medium REEs. Positives anomalies for Eu, Gd, Tb and Lu were highlighted particularly in vertebrates, possibly due to species-dependent bioaccumulation/detoxification or related to anthropogenic inputs. The calculated BAF and BSAF indicated an important partitioning of REEs in organisms compared to the dissolved phase and a limited transfer from sediment to organisms.

Trophic transfer of rare earth elements in the food web of the Loire estuary (France).

The increasing use of rare earth elements (REEs) in many industrial sectors and in medecine, causes discharges into the environment and particularly in estuarine areas subjected to strong anthropogenic pressures. Here, we assessed the distribution of REEs along the food web of the Loire estuary. Several species representative of different trophic levels were sampled: 8 vertebrates, 3 crustaceans, 2 mollusks, 3 annelids and 4 algae, as well as Haploops sp. tubes rather related to sediment. The total REE concentrations measured by ICP-MS were the highest in Haploops sp. tubes (141.1 ± 4.7 µg/g dw), algae (1.5 to 34.5 µg/g dw), mollusks (9.9 to 12.0 µg/g dw), annelids (0.7 to 19.9 µg/g dw) and crustaceans (1.4 to 6.3 µg/g dw) and the lowest in vetebrates (0.1 to 1.6 µg/g dw). The individual contribution of REEs was, however, similar between most studied species with a higher contribution of light REEs (76.7 ± 7.6 %) compared to heavy REEs (14.1 ± 3.7 %) or medium REEs (9.2 ± 5.8 %). Trophic relations were estimated by stable isotope analysis of C and N and the linear regression of δ15N with total REE concentrations highlighted a trophic dilution with a corresponding TMS of -2.0. The tissue-specific bioaccumulation investigated for vertebrates demonstrated a slightly higher REE accumulation in gonads than in the muscle. Finally, positive Eu, Gd, Tb and Lu anomalies were highlighted in the normalized REE patterns of most studied species (especially in fish and crustaceans), which is consistent with results in the dissolved phase for Eu and Gd. These anomalies could either be due to anthropogenic inputs or to various bioaccumulation/elimination processes according to the specific species physiology. This study, including most of the trophic levels of the Loire estuary food web provides new insights on the bioaccumulation and trophic transfer of REEs in natural ecosystems.

Distribution of rare earth elements and assessment of anthropogenic gadolinium in estuarine habitats: The case of Loire and Seine estuaries in France.

Rare earth elements (REEs), attractive to society because of their applications in industry, agriculture and medicine, are increasingly released into the environment especially in industrialized estuaries. This study compared the REE distribution in the abiotic compartments: water (dissolved phase (<0.45 µm), suspended particulate matter (SPM)) and sediment of the Loire and Seine estuaries (France). A total of 8 and 6 sites were investigated in the Loire and Seine, respectively, as well as 5 additional offshore sites for the Loire. Total REE concentrations were higher in the Loire for the dissolved phase (93.5 ± 63.3 vs 87.7 ± 16.2 ng/L), SPM (173.9 ± 18.3 vs 114.0 ± 17.8 mg/kg dw) and sediments (198.2 ± 27.9 vs 73.2 ± 27.4 mg/kg dw), explained by higher geogenic inputs. Individual REE contributions along with normalization highlighted heavy REE enrichments and Gd positive anomalies in the dissolved phase of the two estuaries, whereas REE distributions in SPM and sediments followed the natural abundance of the REE classes. The calculated Gd anomalies in the dissolved phase were higher in the Seine (9.7 ± 3.4) than in the Loire (3.0 ± 0.8), corresponding to 88.3 ± 5.1 % and 64.4 ± 11.1 % of anthropogenic Gd. This demonstrates a higher contamination of the Seine estuary, certainly due to the difference in the number of inhabitants between both areas involving different amounts of Gd used in medicine. The offshore sites of Loire showed lower total REE concentrations (55.8 ± 5.8 ng/L, 26.7 ± 38.2 mg/kg dw and 100.1 ± 11.7 mg/kg dw for the dissolved phase, SPM and sediments, respectively) and lower Gd anomalies (1.2 ± 0.2) corresponding to only 13.3 ± 3.9 % of anthropogenic Gd, confirming a contamination from the watershed. This study comparing two major French estuaries provides new data on the REE distribution in natural aquatic systems.

Vg mRNA induction in an endangered fish species (Anguilla anguilla) from the Loire estuary (France).

Estuarine zones are extremely fragile due to increasing stress from anthropogenic activities. Among those, the Loire estuary (France) is potentially exposed to various contaminants including Endocrine Disruptors Compounds (EDCs) able to impact the reproduction physiology of fish. The European eel (Anguilla anguilla), endangered fish species, is apparently not relevant, in its yellow stage, to monitor the effects of endocrine disruption. Despite this weakly responsiveness, this study aimed to investigate whether European eel from the Loire estuary may still be the subject of estrogenic disruption quantifying the hepatic Vg gene expression according to gender and sexual stage. Vitellogenin (Vg) appears as a valuable biomarker of EDCs, as well as for exposure and effects. Quantitative real-time Reverse Transcription Polymerase Chain Reaction (q RT PCR) was used in this study to amplify responses of hepatic Vg transcripts. European eels were sampled in May 2009 (N=57) and November 2010 (during the downstream migration, N=10) in two sites of the Loire estuary with different ecological conditions and contamination pressures (upstream: Varades; downstream: Nantes). Reproductive (gender, sexual maturity stage) and biometric parameters of collected eels were determined. A laboratory exposure of silver male to steroid hormones (Testosterone (T), 11-KetoTestosterone (11-KT), Estradiol (E2)) was conducted in parallel to validate the q RT PCR approach on hepatic Vg mRNA. Results demonstrated the responsiveness of exposed silver male eels, since hepatic mRNA Vg induction was observed in E2 treated males compared to control specimens. In the field, results of female silver eels reflected large inter-individual differences in the activation of hepatic Vg at silvering. However, while only female silver eels should express hepatic Vg mRNA, quantifiable levels were also detected in a proportion of 38% of the other individuals sampled, normally not inclined to express it, those being undifferentiated eels, yellow females, yellow and silver males. According to each sexual stage, no difference of expression was observed between eels from the two sampling sites. Histological results as well as low Vg mRNA levels detected do not permit a conclusion as to a potential effect of endocrine disruption.

Unprecedented 17-residue peptaibiotics produced by marine-derived Trichoderma atroviride.

In the course of investigations on marine-derived toxigenic fungi, five strains of Trichoderma atroviride were studied for their production of peptaibiotics. While these five strains were found to produce classical 19-residue peptaibols, three of them exhibited unusual peptidic sodium-adduct [M + 2 Na](2+) ion peaks at m/z between 824 and 854. The sequencing of these peptides led to two series of unprecedented 17-residue peptaibiotics based on the model Ac-XXX-Ala-Ala-XXX-XXX-Gln-Aib-Aib-Aib-Ala/Ser-Lxx-Aib-Pro-XXX-Aib-Lxx-[C(129) ]. The C-terminus of these new peptides was common to all of them, and its elemental formula C5 H9 N2 O2 was established by HR-MS. It could correspond to the cyclized form of N(δ) -hydroxyornithine which has already been observed at the C-terminus of various peptidic siderophores. The comparison of the sequences of 17- and 19-residue peptides showed similarities for positions 1-16. This observation seems to indicate a common biosynthesis pathway. Both new 17-residue peptaibiotics and 19-residue peptaibols exhibited weak in vitro cytotoxicities against KB cells.

From geochemistry to ecotoxicology of rare earth elements in aquatic environments: Diversity and uses of normalization reference materials and anomaly calculation methods.

The geochemistry of rare earth elements (REEs) has been studied for a long time and has allowed us to highlight enrichments or depletions of REEs in aquatic ecosystems and to estimate anthropogenic inputs through normalization of data to different reference materials. This review of current literature on REE normalization highlighted the large number of different reference materials (a total of 12), as well as different anomaly calculation methods. This statement showed a real need for method harmonization to simplify the comparison between studies, which is currently very difficult. Normalization to Post-Archean Australian Shale (PAAS) emerged as being the most used (33 % of reported studies) regardless of the location and the nature of the studied samples and seem to be of higher quality. The interest of other reference materials was nevertheless underlined, as they could better represent the geographical situation or the nature of samples. Two main anomaly calculation methods have been highlighted: the linear interpolation/extrapolation and the geometric extrapolation using logarithmic modeling. However, due to variations in the estimation of neighbors' values, these two methods produce many different equations for the anomaly calculation of a single element. Current normalization practices based on shales and chondrites are suitable for abiotic samples but are questionable for biota. Indeed, normalization is increasingly used in studies addressing ecotoxicological issues which focus on biota and often aim to estimate the anthropogenic origin of bioaccumulated REEs. Due to the interspecific variability, as well as the complexity of mechanisms occurring in organisms when exposed to contaminants, new reference materials need to be established to consider the bioaccumulation/metabolization processes and the anthropogenic inputs of REEs based on the results of biotic samples.

Study of the ageing and the sorption of polyaromatic hydrocarbons as influencing factors on the effects of microplastics on blue mussel.

The mussels are species with high socio-economic weights and are often used as bioindicators of biological and chemical contamination. In the field and aquaculture, they can intake microplastics during filter-feeding, and the microplastics can have a negative impact on their health, even at low concentrations. The effects of microplastics have yet to be fully examined on the blue mussel (Mytilus edulis), considering the factors of ageing and sorption of some polyaromatic hydrocarbons (PAHs), ubiquitous environmental contaminants. In this work, 5 different exposure conditions were studied: pristine microplastics, microplastics aged for 1000 days under UV radiation, microplastics sorbing PAHs, as well as microplastics both aged and sorbing PAHs, in parallel to controls. The microplastic changes after ageing were studied with spectroscopic and chromatographic methods. Then, 8-day laboratory exposures of mussels at 10 µg/L of microplastics were performed. The oxidative stress, as well as neurotoxic and immunological responses of M. edulis, were measured using a battery of biomarkers (catalase/CAT, superoxide dismutase/SOD, glutathione S-transferases/GST, acetylcholinesterase/AChE) in 3 different organs (digestive gland, gills and mantle), and acid phosphatase in hemolymph. Then, a study of lipid impairments on the digestive gland was performed through the use of lipidomic tools. No significant difference of oxidative stress activity was observed for all the tissues of mussels exposed to pristine microplastics at 10 µg/L, compared to controls. The ageing and the PAH soption onto microplastics were influencing factors of the oxydative stress in mussels with increased CAT activities in the digestive glands and decreased SOD activities in the mantles. The neurotoxicity was highlighted by higher AChE activities measured in the mantle of mussels exposed to all the microplastic treatments, compared to controls. Concerning lipidomics, no compound was determined as a biomarker of microplastic exposure. The study demonstrated a low toxicity of microplastics at environmental relevant concentration with a 8-day exposure and using the chosen biomarkers. However, some microplastic changes seemed to lead to specific effects on mussels.

Rare earth element organotropism in European eel (Anguilla anguilla).

Rare earth elements (REEs) are metallic elements with electronic, magnetic, optical and catalytic properties which make them essential in many industrial and medical fields. REEs are therefore becoming emerging pollutants and it is important to understand their implications for ecosystem health. However, little knowledge of REE bioaccumulation in aquatic organisms is available and especially on their internal distribution in fish. In the present study, REE organotropism was determined in Anguilla anguilla from the Loire estuary (France) by determining burdens in a wide set of tissues, organs and biological fluids. Differences have been observed between life stages and genders. For yellow eels, the most accumulating organ was the gills (126.90 ± 50.78 µg/kg dw) and for silver eels, it was the liver (181.78 ± 62.04 µg/kg dw for males; 203.79 ± 111.86 µg/kg dw for females). The comparison between female silver and yellow eels shown that female silver individuals accumulated significantly more REEs in the urinary system (US), muscles, gonads, spleen and liver, while yellow individuals accumulated more in gills. The comparison between male and female silver eels also highlighted differences, indeed the females accumulated significantly more REEs in the US, gonads, skin and spleen, compared to males which accumulated significantly more in muscles and gills. REEs abundances are also different between organs, life stages and genders. The gonads of female silver eels exhibited a particular profile with the dominance of gadolinium (Gd) (up to 74.2% of ∑REEs). Moreover, the presence of Anguillicola crassus in the swim bladder of organisms seemed to have an impact on REE bioaccumulation: parasitized yellow eels present higher concentrations of REEs in muscles, gills, gonads and liver than non-parasitized individuals. Regarding glass eels, REE contribution profiles in the whole body were close to those of yellow and silver eel skin.

Are bio-based and biodegradable microplastics impacting for blue mussel (Mytilus edulis)?

The substitution of petrochemical plastics by bio-based and biodegradable plastics are in need of an evaluation for the potential toxic impacts that they can have on marine wildlife. This study aims to assess the toxicological effects of polylactic acid microparticles at two concentrations, 10 and 100 µg/L, during 8 days on the blue mussel, Mytilus edulis. No significant oxidative stress (catalase, glutathione-S-transferase and superoxide dismutase activities), neurotoxicity (acetylcholinesterase), or immunotoxicity (lysosomal membrane stability and acid phosphatase activity) were detectable. The multivariate analysis of metabolomic data allowed us to differentiate the individuals according to the exposure. From the loading plot of OPLS-DA, 48 ions down-regulated in the individuals exposed to microplastics. They were identified based on HRMS data as glycerophospholipids.

Rare earth element bioaccumulation in the yellow and silver European eel (Anguilla anguilla): A case study in the Loire estuary (France).

In the present work, rare earth elements (REEs) were measured in European eel muscles (Anguilla anguilla) from the Loire estuary in France. This study site is characterized by a large anthropogenic pressure with potential activities releasing REEs such as oil refineries, aeronautic and naval industries, wind turbine industries, hospitals with magnetic resonance imaging and coal-fired power plants. These activities may lead to increased REE concentrations in sediments the primary habitat of European eels. In the present work, REE bioaccumulation was evaluated by determining the concentrations in yellow and silver eel muscles sampled at three different locations in the Loire estuary and at two periods (2011/2012 and 2018/2019). The aims of this study were the understanding of the spatio-temporal influences (sampling site and sampling period) and intraspecific variations (age, sex, sexual maturation, length, weight, and parasitism) on the whole REE bioaccumulation. The mean value of the sum of REE concentrations (∑REEs) was 2.91, 6.48 and 12.60 µg/kg of muscle from respectively yellow eels, female silver eels and male silver eels fished in 2018/2019. The results showed that silver males accumulated more REEs than silver females and silver eels accumulate more REEs than yellow ones. Regarding the determination of spatio-temporal variations, an increase of REE concentrations for silver eel muscles between the two periods was observed, certainly related to the increase of REE uses. Finally, a trend of higher contamination of eels sampled in the downstream of Nantes was noticed for yellow eels.

Factors influencing the microplastic contamination of bivalves from the French Atlantic coast: Location, season and/or mode of life?

Monitoring the presence of microplastics (MP) in marine organisms is currently of high importance. This paper presents the qualitative and quantitative MP contamination of two bivalves from the French Atlantic coasts: the blue mussel (Mytilus edulis) and the Pacific oyster (Crassostrea gigas). Three factors potentially influencing the contamination were investigated by collecting at different sampling sites and different seasons, organisms both wild and cultivated. Inter- and intra-species comparisons were also achieved. MP quantity in organisms was evaluated at 0.61±0.56 and 2.1±1.7MP per individual respectively for mussels and oysters. Eight different polymers were identified. Most of the MPs were fragments; about a half of MPs were grey colored and a half with a size ranging from 50 to 100µm for both studied species. Some inter-specific differences were found but no evidence for sampling site, season or mode of life effect was highlighted.

Microplastic abundance and characteristics in French Atlantic coastal sediments using a new extraction method.

The ubiquitous presence of microplastics (MPs) has been demonstrated in all environmental compartments in the recent years. They are detected in air, freshwater, soil, organisms and particularly in marine ecosystems. Since sediments are known to be the major sink of many organic and inorganic pollutants, the aim of this study was to develop and validate a fast and cheap methodology to assess the MP contamination in intertidal sediments from the Gulf of Biscay (Pays de la Loire region, France). Sediments were sampled at three locations (Pays de la Loire region, France) and during two seasons: October 2015 and March 2016. The analytical protocol involved MP extraction from dried sediments using milliQ water and a centrifugation technique. After a filtration step of supernatants, MPs were detected and directly identified on the membrane filters using µFTIR spectroscopy in reflection mode. For the first time, the number of replicates allowing to obtain a satisfying representativeness of the whole sampled sediment was also evaluated at 10 replicates of 25 g each. The average number of MPs in sediments was 67 (±76) MPs/kg dw (N = 60) with no significant difference between sites and seasons. Ten different compositions of MPs were defined by µFT-IR with a high proportion of polypropylene (PP) and polyethylene (PE), 38 and 24%, respectively. Among MPs, mainly fragments (84%) were observed with main size classes corresponding to [>100 µm] and [50-100 µm] but no particles > 1 mm could be found suggesting that mainly small microplastics (<1 mm) were subject to vertical transport.

Quantification and characterization of microplastics in blue mussels (Mytilus edulis): protocol setup and preliminary data on the contamination of the French Atlantic coast.

Microplastics (MPs) constitute a main environmental issue due to their threat to marine organisms and so far to humans. The lack of a fast standard protocol in MP isolation and identification from living organisms bring to challenge for the science. In this paper, an optimized protocol using potassium hydroxide 10% (KOH 10%; m/v) for digestion of mussel soft tissues (Mytilus edulis) and multi-steps of sedimentation has been developed. Efficiency higher than 99.9% of organic and mineral matter elimination was shown by application on mussels sampled on the French Atlantic coast. The identification of MPs was performed by FTIR microscopy straight on the filter and the whole analysis can be compatible with a routine goal. Fourteen MPs of four different chemical natures were found and identified in 5 pools of 3 sampled mussels. Their size ranged from 30 to 200 µm. Further investigations are now needed to evaluate the potential risk of such particles within this marine bivalve species and other filter feeders.

Is there any consistency between the microplastics found in the field and those used in laboratory experiments?

The ubiquitous presence and persistency of microplastics (MPs) in aquatic environments are of particular concern since they represent an increasing threat to marine organisms and ecosystems. Great differences of concentrations and/or quantities in field samples have been observed depending on geographical location around the world. The main types reported have been polyethylene, polypropylene, and polystyrene. The presence of MPs in marine wildlife has been shown in many studies focusing on ingestion and accumulation in different tissues, whereas studies of the biological effects of MPs in the field are scarce. If the nature and abundance/concentrations of MPs have not been systematically determined in field samples, this is due to the fact that the identification of MPs from environmental samples requires mastery and execution of several steps and techniques. For this reason and due to differences in sampling techniques and sample preparation, it remains difficult to compare the published studies. Most laboratory experiments have been performed with MP concentrations of a higher order of magnitude than those found in the field. Consequently, the ingestion and associated effects observed in exposed organisms have corresponded to great contaminant stress, which does not mimic the natural environment. Medium contaminations are produced with only one type of polymer of a precise sizes and homogenous shape whereas the MPs present in the field are known to be a mix of many types, sizes and shapes of plastic. Moreover, MPs originating in marine environments can be colonized by organisms and constitute the sorption support for many organic compounds present in environment that are not easily reproducible in laboratory. Determination of the mechanical and chemical effects of MPs on organisms is still a challenging area of research. Among the potential chemical effects it is necessary to differentiate those related to polymer properties from those due to the sorption/desorption of organic compounds.

Development and validation of LC-MS methods for peptaibol quantification in fungal extracts according to their lengths.

Some terrestrial Trichoderma sp. strains are already used as biological control agents (BCAs). They all produce peptaibols, small antimicrobial peptides which are supposed to play a role in the anti-phytopathogenic activity of Trichoderma sp. Trichoderma strains producing high amounts of peptaibols could represent new potential BCAs. In this context, marine-derived Trichoderma strains from the marine fungal strain collection of the "Mer, Molécules, Santé" (MMS) laboratory were investigated for their peptaibol production. Previously, the quantification of peptaibols was performed using alamethicin, as standard (20-amino acid residues peptaibol). In this study, the development and validation of quantification LC/ESI-TI-MS methods using different standards of peptaibols (11-, 14- and 20-amino acid residues) was performed in order to quantify all of them, in a single analysis, in Trichoderma crude extracts according to their chain length. The developed and validated methods were used to study the peptaibol production kinetic of a marine-derived Trichoderma strain, i.e., Trichoderma longibrachiatum (MMS 151). The results showed the optimal culture time at the 9th day with concentrations reaching 1.4±0.2% and 2.3±0.4% of the fungal biomass respectively for 11- and 20-residue peptaibols. Then, the different peptaibol subgroups produced by 13 Trichoderma strains were quantified. According to their 18-, 19- and 20-residue peptaibol production, three strains referenced as MMS 1541, MMS 639 and MMS 151 seemed to be good candidates as potential new biological control agents with respective production of 0.4, 0.4 and 2.1%.

The influence of salinity on the fate and behavior of silver standardized nanomaterial and toxicity effects in the estuarine bivalve Scrobicularia plana.

Because of their antibacterial properties, silver (Ag) engineered nanomaterials are included in many products. The present study used a standardized Ag nanomaterial (NM-300K, 20 nm) supplied with a stabilizing agent. The aim was to investigate the behavior of Ag nanomaterial in an estuarine-like medium at 2 salinities (15 psu and 30 psu). Uptake as well as sublethal effects of Ag nanomaterial (10 µg Ag/L), its stabilizing agent, and AgNO3 (10 µg Ag/L) were assessed in the clam Scrobicularia plana, after 7 d of exposure. The release of soluble Ag from Ag nanomaterial in the experimental media was quantified by using diffusive gradient in thin films and ultrafiltration. A multibiomarker approach was employed to reveal responses of clams at subindividual and individual levels. The bioaccumulation of Ag was significantly greater at 15 psu versus 30 psu, which could be explained by differences in Ag speciation. In conclusion, the present study showed different impacts of Ag nanomaterial that were not always explained by the release of Ag ions in clams at both salinities; such impacts were particularly characterized by induction of oxidative stress, cell damage, and impairment of energetic levels. Burrowing of clams was affected by the stabilizing agent depending on the salinity tested, with stronger effects at 15 psu. Finally, the present study highlighted salinity-dependent changes in the physiology of estuarine bivalves. Environ Toxicol Chem 2016;35:2550-2561. © 2016 SETAC.

Cadmium sulfide quantum dots induce oxidative stress and behavioral impairments in the marine clam Scrobicularia plana.

Cadmium sulfide (CdS) quantum dots have a number of current applications in electronics and solar cells and significant future potential in medicine. The aim of the present study was to examine the toxic effects of CdS quantum dots on the marine clam Scrobicularia plana exposed for 14 d to these nanomaterials (10 µg Cd L(-1) ) in natural seawater and to compare them with soluble Cd. Measurement of labile Cd released from CdS quantum dots showed that 52% of CdS quantum dots remained in the nanoparticulate form. Clams accumulated the same levels of Cd regardless of the form in which it was delivered (soluble Cd vs CdS quantum dots). However, significant changes in biochemical responses were observed in clams exposed to CdS quantum dots compared with soluble Cd. Increased activities of catalase and glutathione-S-transferase were significantly higher in clams exposed in seawater to Cd as the nanoparticulate versus the soluble form, suggesting a specific nano effect. The behavior of S. plana in sediment showed impairments of foot movements only in the case of exposure to CdS quantum dots. The results show that oxidative stress and behavior biomarkers are sensitive predictors of CdS quantum dots toxicity in S. plana. Such responses, appearing well before changes might occur at the population level, demonstrate the usefulness of this model species and type of biomarker in the assessment of nanoparticle contamination in estuarine ecosystems.

Neurodevelopmental and behavioral effects of nonylphenol exposure during gestational and breastfeeding period on F1 rats.

Nonylphenols (NP) are endocrine-disruptors known to be widely present in our environment. This study evaluated the effects of 4-n-NP on neurobehavioral development and memory capacity after perinatal exposure on the offspring rats. Dams were gavaged with 4-n-NP (0, 50 and 200mg/kg/day) from gestational day 5 to postnatal day (PND) 21. Dams exposed to the higher dose lost weight during gestation and had a longer gestational duration. Juvenile female pups of the 200mg 4-n-NP/kg/day group were lighter. Their thyroid somatic index (TSI) was also affected. For male pups, a decrease of TSI at weaning for the 200mg 4-n-NP/kg/day group and an increase of GSI for the 50mg 4-n-NP/kg/day group were observed. Physical maturation (incisives and eyes) were likewise affected. In open field (OF) tests, females were more active than males. In the first OF (PND 36), a treatment effect was observed only for males, particularly for the high dose group, which became as active as females. The second OF (PND 71) showed few differences between groups (treated vs control), the gender difference whatever the dose was not abolished. In the Morris Water Maze test, the study of the first 30s showed that females (200mg/kg/day) were mainly affected. Their performances were improved by 4-n-NP. These effects were particularly important for the first short-term memory test and observed to a lesser extent in the second evaluation of the long-term memory (PND 69). These data showed that perinatal 4-n-NP exposure induced behavioral and neuro-developmental impairments from 50mg/kg/day.