RESUMO
BACKGROUND: Broodstock nutritional programming improves the offspring utilization of plant-based diets in gilthead sea bream through changes in hepatic metabolism. Attention was initially focused on fatty acid desaturases, but it can involve a wide range of processes that remain largely unexplored. How all this can be driven by a different genetic background is hardly underlined, and the present study aimed to assess how broodstock nutrition affects differentially the transcriptome and genome-wide DNA methylome of reference and genetically selected fish within the PROGENSA® selection program. RESULTS: After the stimulus phase with a low fish oil diet, two offspring subsets of each genetic background received a control or a FUTURE-based diet. This highlighted a different hepatic transcriptome (RNA-seq) and genome-wide DNA methylation (MBD-seq) pattern depending on the genetic background. The number of differentially expressed transcripts following the challenge phase varied from 323 in reference fish to 2,009 in genetically selected fish. The number of discriminant transcripts, and associated enriched functions, were also markedly higher in selected fish. Moreover, correlation analysis depicted a hyper-methylated and down-regulated gene expression state in selected fish with the FUTURE diet, whereas the opposite pattern appeared in reference fish. After filtering for highly represented functions in selected fish, 115 epigenetic markers were retrieved in this group. Among them, lipid metabolism genes (23) were the most reactive following ordering by fold-change in expression, rendering a final list of 10 top markers with a key role on hepatic lipogenesis and fatty acid metabolism (cd36, pitpna, cidea, fasn, g6pd, lipt1, scd1a, acsbg2, acsl14, acsbg2). CONCLUSIONS: Gene expression profiles and methylation signatures were dependent on genetic background in our experimental model. Such assumption affected the magnitude, but also the type and direction of change. Thus, the resulting epigenetic clock of reference fish might depict an older phenotype with a lower methylation for the epigenetically responsive genes with a negative methylation-expression pattern. Therefore, epigenetic markers will be specific of each genetic lineage, serving the broodstock programming in our selected fish to prevent and mitigate later in life the risk of hepatic steatosis through changes in hepatic lipogenesis and fatty acid metabolism.
Assuntos
Dourada , Animais , Dourada/genética , Dourada/metabolismo , Transcriptoma , Epigenoma , Ácidos Graxos Dessaturases/genética , Ácidos Graxos/metabolismoRESUMO
The aim of this study was to assess the effects of low levels of dietary fish meal (FM) and fish oil (FO) on disease resistance and gut associated lymphoid tissue (GALT) response after an experimental intestinal infection with V. anguillarum in European sea bass (Dicentrarchus labrax) For that purpose, sea bass juveniles were fed one of four diets containing combined levels of FO and FM as follows: 20%FM/6%FO, 20%FM/3%FO, 5%FM/6%FO and 5%FM/3%FO during 153 days. At the end of the feeding trial, fish were subjected to either an in vivo exposure to a sub-lethal dose of V. anguillarum via anal inoculation or to an ex vivo exposure to V. anguillarum. Additionally, inducible nitric oxide synthase (iNOS) and tumor necrosis factor α (TNFα) gut patterns of immunopositivity were studied. Growth performance was affected by dietary FM level, however ex vivo gut bacterial translocation rates and survival after the in vivo challenge test were affected by dietary FO level. After 5 months of feeding, low dietary FM levels led to a posterior gut up-regulation of interleukin-1ß (IL-1ß) and TNFα, major histocompatibility complex-II (MHCII) and cyclooxygenase-2 (COX2), which in turn reduced the gut associated lymphoid tissue (GALT) capacity of response after 24 h post infection and conditioned European sea bass capacity to recover gut homeostasis 7 days post infection. Immunoreactivity to anti-iNOS and anti-TNFα presented a gradient of increased immunopositivity towards the anus, regardless of the dietary FM/FO fed. Strong positive anti-TNFα isolated enterocytes were observed in the anterior gut in relation to low levels of dietary FM/FO. Submucosa and lamina propria immunoreactivity grade was related to the amount of leucocyte populations infiltrated and goblet cells presented immunopositivity to anti-iNOS but not to anti-TNFα. Thus, reducing FO content from 6% to a 3% by VO in European sea bass diets increases ex vivo and in vivo gut bacterial translocation rates, whereas reducing FM content from 20% down to 5% up-regulates the expression of several posterior gut inflammation-related genes conditioning fish growth and GALT capacity of response after bacterial infection.
Assuntos
Bass/imunologia , Suplementos Nutricionais , Resistência à Doença , Doenças dos Peixes/imunologia , Óleos de Peixe/imunologia , Enteropatias/veterinária , Vibrioses/veterinária , Ração Animal , Animais , Dieta/veterinária , Enteropatias/imunologia , Vibrio/fisiologia , Vibrioses/imunologiaRESUMO
Lack of operculum, a neurocranial deformity, is the most common external abnormality to be found among industrially produced gilthead seabream (Sparus aurata L.), and this entails significant financial losses. This study conducts, for the first time in this species, a quantitative trait loci (QTL) analysis of the lack of operculum. A total of 142 individuals from a paternal half-sibling family (six full-sibling families) were selected for QTL mapping. They had previously shown a highly significant association with the prevalence of lack of operculum in a segregation analysis. All the fish were genotyped for 106 microsatellite markers using a set of multiplex PCRs (ReMsa1-ReMsa13). A linear regression methodology was used for the QTL analysis. Four QTL were detected for this deformity, two of which (QTLOP1 and QTLOP2) were significant. They were located at LG (linkage group) nine and LG10 respectively. Both QTL showed a large effect (about 27%), and furthermore, the association between lack of operculum and sire allelic segregation observed was statistically significant in the QTLOP1 analysis. These results represent a significant step towards including marker-assisted selection for this deformity in genetic breeding programmes to reduce the incidence of the deformity in the species.
Assuntos
Encéfalo/anormalidades , Doenças dos Peixes/genética , Locos de Características Quantitativas , Dourada/anormalidades , Dourada/genética , Animais , Cruzamento , Mapeamento Cromossômico , Feminino , Ligação Genética , Modelos Lineares , Masculino , Repetições de Microssatélites , Linhagem , FenótipoRESUMO
One of the most important problems of fish aquaculture is the high incidence of fish deformities, which are mainly skeletal. In this study, genetic parameters on gilthead seabream (Sparus aurata L.) for skeleton deformities at different ages (179, 269, 389, 539 and 689 days) and their correlations with growth traits were estimated, as were as their genotype × environment interactions (G × E) at harvesting age. A total of 4093 offspring from the mass spawning of three industrial broodstocks belonging to the PROGENSA(®) breeding programme were mixed and on-grown by different production systems in four Spanish regions: Canary Islands (tanks and cage), Andalusia (estuary), Catalonia (cage) and Murcia (cage). Parental assignment was inferred using the standardized SMsa1 microsatellite multiplex PCR. From three broodstocks, 139 breeders contributed to the spawn and a total of 297 full-sibling families (52 paternal and 53 maternal half-sibling families) were represented. Heritabilities at different ages were medium for growth traits (0.16-0.48) and vertebral deformities (0.16-0.41), and low for any type of deformity (0.07-0.26), head deformities (0.00-0.05) and lack of operculum (0.06-0.11). The genetic correlations between growth and deformity traits were medium and positive, suggesting that to avoid increasing deformities they should be taken into account in breeding programmes when growth is selected. The G × E interactions among the different facilities were weak for length and deformity and strong for growth rate during this period. These results highlight the potential for the gilthead seabream industry to reduce the prevalence of deformities by genetic improvement tools.
Assuntos
Osso e Ossos/anormalidades , Interação Gene-Ambiente , Genótipo , Dourada/crescimento & desenvolvimento , Dourada/genética , Envelhecimento , Animais , Aquicultura/métodos , Cruzamento , Repetições de Microssatélites , Característica Quantitativa Herdável , EspanhaRESUMO
The high number of multiplex PCRs developed for gilthead seabream (Sparus aurata L.) from many different microsatellite markers does not allow comparison among populations. This highlights the need for developing a reproducible panel of markers, which can be used with safety and reliability by all users. In this study, the first standardised panel of two new microsatellite multiplex PCRs was developed for this species. Primers of 138 specific microsatellites from the genetic linkage map were redesigned and evaluated according to their genetic variability, allele size range and genotyping reliability. A protocol to identify and classify genotyping errors or potential errors was proposed to assess the reliability of each marker. Two new multiplex PCRs from the best assessed markers were designed with 11 markers in each, named SMsa1 and SMsa2 (SuperMultiplex Sparus aurata). Three broodstocks (59, 47 and 98 breeders) from different Spanish companies, and a sample of 80 offspring from each one, were analysed to validate the usefulness of these multiplexes in the parental assignation. It was possible to assign each offspring to a single parent pair (100% success) using the exclusion method with SMsa1 and/or SMsa2. In each genotyped a reference sample (Ref-sa) was used, and its DNA is available on request similar to the kits of bin set to genotype by genemapper (v.3.7) software (kit-SMsa1 and kit-SMsa2). This will be a robust and effective tool for pedigree analysis or characterisation of populations and will be proposed as an international panel for this species.
Assuntos
Reação em Cadeia da Polimerase Multiplex/métodos , Dourada/genética , Animais , Aquicultura/métodos , Aquicultura/normas , Repetições de Microssatélites , Reação em Cadeia da Polimerase Multiplex/normasRESUMO
Morphological abnormalities in farmed gilthead seabream (Sparus aurata) are a major problem as it entails significant economic losses. In this study, 3 large scale experiments under different conditions of spawning, offspring handling and breeders phenotype were performed to analyze the inheritance of 4 types of deformities in this species: lack of operculum, lordosis, vertebral fusion, which are 3 of the most important skeletal deformities, and LSK, which is a consecutive repetition of lordosis/scoliosis/kyphosis. In Exp. [1] (mass spawning and fingerling sorting), 900 fish were analyzed at 509 d post-hatching: 846 fish that had been on-grown in a farm and 54 LSK-deformed fish that had been reared separately after being selected during the fingerling sorting process. A total of 89 families were represented. A statistically significant association between 5 of these families (from 6 breeders) and LSK-deformed fish was found. In Exp. [2] (mass spawning and no fingerling sorting), 810 fish were analyzed at 2 ages: 179 and 689 d post-hatching. Significant relationships between 2 of the breeders and 2 of the families with the lack of operculum prevalence of their descendants were found at 689 d but not at 179 d. Heritabilities: 0.09 ± 0.09 at 179 d and 0.17 ± 0.08 at 689 d. Column deformities prevalence was low and no association with family was observed. Family relationships were determined by microsatellites multiplex PCR in both experiments. In Exp. [3] (designed mating), sires suffering from lordosis or lack of operculum or vertebral fusion deformities were mated with non-deformed dams and a mass-spawning mating was considered as a control. After analyzing 11,503 offspring at 159 d post-hatching, a significant relationship between each deformity prevalence and the mating of breeders suffering from the same deformity was observed. In addition, a significant prevalence of lack of operculum in offspring from lordotic matings was observed. Heritabilities ranged from 0.34 to 0.46 for the 3 deformities. The results of the present study suggest that these deformities have a genetic origin. They also suggest that the sorting process is not recommended and that producers should consider these deformities in genetic breeding programs to significantly improve their fish morphological quality and to minimize farmed fish deformities incidence.
Assuntos
Doenças dos Peixes/genética , Lordose/veterinária , Dourada/anormalidades , Dourada/genética , Doenças da Coluna Vertebral/veterinária , Animais , Cruzamento , Lordose/genética , Reprodução , Doenças da Coluna Vertebral/genética , Doenças da Coluna Vertebral/patologia , Coluna Vertebral/anormalidadesRESUMO
109 patients older than seventy years of age and operated of gallstone disease in our service during a period of 4.5 years are presented. The average age of the series was 78.8 years, with a male/female (M/F) ratio of 1/1.6. 77% of the patients were admitted on an emergency basis due to a complication derived from the gallstone disease they were suffering. Only 39.8% of the patients were previously diagnosed of gallstone disease at the time of admission and 10% presented an associated surgical condition which was treated simultaneously. Overall morbidity of the series was 36%, with a mortality of 2 cases (1.9%).
Assuntos
Procedimentos Cirúrgicos do Sistema Biliar , Idoso , Procedimentos Cirúrgicos do Sistema Biliar/mortalidade , Procedimentos Cirúrgicos do Sistema Biliar/estatística & dados numéricos , Colelitíase/epidemiologia , Colelitíase/mortalidade , Colelitíase/cirurgia , Emergências , Humanos , Complicações Pós-Operatórias/epidemiologia , Estudos Retrospectivos , Fatores de Risco , Fatores Sexuais , Espanha/epidemiologiaRESUMO
En los últimos doce años se han desarrollado unconjunto considerable de investigaciones acerca deldesarrollo de la teoría de la mente (TM) en niñossordos, que revelan un importante retraso en lacomprensión de la falsa creencia en los niños sordoshijos de padres oyentes. En este trabajo se presentandatos de una investigación española que evalúala relación entre edad, lenguaje y comprensión de lafalsa creencia de primer y segundo orden en sujetossignantes tardíos. La muestra está formada por 54participantes sordos (entre 6 y 19 años) procedentesde familias oyentes. Todos los participantes presentansordera prelocutiva severa o profunda sindeficiencias asociadas. Los resultados muestran unretraso importante en relación con el desarrollotípico, pues es a partir de los 11 años cuando losparticipantes muestran altos porcentajes de éxitoen la resolución de las tareas de falsa creencia. Lacomprensión de falsa creencia de segundo orden seobserva con más frecuencia en participantes de 14años en adelante. Además de la edad, el nivel decompetencia léxica oral es una variable que explicalas diferencias entre los participantes sordos respectoa su ejecución en TM. En la discusión se reflexionasobre estos datos y se aportan principios einstrumentos para guiar la intervención en TM conniños sordos, tanto por parte de los profesionalescomo del entorno familiar
In the past twelve years a considerable amount ofresearch has been carried out on the developmentof theory of mind (ToM) in deaf children, reportingthat deaf children with hearing parents present aserious delay in the understanding of false belief.In this article, findings from a Spanish study evaluatingthe relation between age, language andunderstanding of first and second-order falsebelief for late-signing deaf participants are shown.The sample consisted of 54 deaf participants (aged6 to 19 years) from hearing families. All the participants present prespeech deafness (severe ordeep) having no associated deficiencies. Theresults show an important delay in ToM in relationwith typical development, since only from 11 yearsold onwards is there a high percentage of successin the resolution of false belief tasks. The understanding of second-order false belief is more frequent in 14 years old onwards participants.Besides age, the level of oral lexical competenceis a variable able to explain the differences in ToMperformance among deaf participants. In Discussionall these data are discussed and some rudimentsfor intervention on ToM with deaf childrenare given to professionals and families