Effects of 7,12-Dimethylbenz(a)anthracene on Apoptosis of Breast Cancer Cells through Regulating Expressions of FasL and Bcl-2.

To provides a reference basis for the apoptosis of breast cancer (BC) cells and the carcinogenesis of BC, the effects of 7, 12-dimethylbenz (a) anthracene (DMBA) on apoptosis regulators FasL and B-cell lymphoma-2 (Bcl-2) were investigated. In this study, 62 female C57BL/6 mice aged from 4 to 6 weeks were randomly divided into control group (CG) and test group (TG), with 31 mice in each group. The TG was given DMBA solution by gavage at a dose of 50 mg/kg, and the CG was given normal saline of equal volume. On the second day after the experiment, all the mice were killed by cervical dislocation. The morphology of the mammary gland was observed by hematoxylin-eosin (HE) staining, and the differences of FasL and Bcl-2 protein expression (PE) were detected by immunohistochemistry. The mRNA expression levels of FasL and Bcl-2 were detected by quantitative real-time PCR (qPCR). Breast cell apoptosis status of mice in the two groups was detected by the terminal deoxyribonucleotidyl transferase (TdT)-mediated biotin-16-dUTP nick-end labelling (TUNEL) method. The results showed that after HE staining, the tumor cells in the TG were stacked up to form a substantial structure. The expression level of FasL protein in the CG was greatly lower than that in the TG, and the positive rate (PR) was 20.25%, which was greatly lower than that of 89.65% in the TG (P<0.01). The expression level of Bcl-2 protein in the mammary gland tissues (MGTs) of mice in the TG was greatly higher than that of the CG, and its PR was 87.96%, which was greatly higher than that of 31.48% in the CG (P<0.01). The expression levels of FasL mRNA in the MGTs of mice in the TG and CG were 5.82±4.37 and 1.27±0.12, respectively, and there was a statistically obvious difference (P<0.05). The mRNA expression levels of Bcl-2 in the TG and the CG were 18.97±2.65 and 2.02±0.54, respectively, and there was an extremely obvious difference (P<0.01). The apoptosis rate of mammary gland cells in the TG was (19.79±3.53) %, and that in the CG was (2.93±0.28) %, and there was an extremely obvious difference (P<0.01). It indicated that DMBA inhibited the apoptosis of BC cells by regulating the up-regulation of FasL and Bcl-2 expression.

Association Between Overt Hyperthyroidism and Risk of Sexual Dysfunction in Both Sexes: A Systematic Review and Meta-Analysis.

BACKGROUND: Numerous studies have shown the detrimental effects of overt hyperthyroidism on sexual functioning but a quantitative result has not yet been synthesized. AIM: To conduct a systematic review and meta-analysis that quantifies the association between overt hyperthyroidism and the risk of sexual dysfunction (SD). METHODS: A meta-analysis of studies in the literature published prior to February 1, 2020, from 4 electronic databases (MEDLINE, Embase, Cochrane Library databases, and PsychINFO) was conducted. All analyses were performed using the random-effects model comparing individuals with and without overt hyperthyroidism. OUTCOMES: The strength of the association between overt hyperthyroidism and risk of SD was quantified by calculating the relative risk (RR) and the standard mean difierences with 95% CI. The quality of evidence for the reported outcome was based on the Grading of Recommendations Assessment, Development, and Evaluation approach. RESULTS: Of 571 publications, a total of 7 studies involving 323,257 individuals were included. Synthetic results from 7 eligible studies indicated that overt hyperthyroidism led to significant SD in both sexes (pooled RR = 2.59, 95% CI: 1.3-5.17, P = .007; heterogeneity: I2 = 98.8%, P < .001). When we analyzed the data of men and women independently, the pooled results consistently showed that men and women with overt hyperthyroidism were at over 2-fold higher risk of SD than the general populations (RR for males = 2.59, 95% CI: 1.03-6.52, P = .044; RR for females = 2.51, 95% CI: 1.47-4.28, P = .001). Combined standard mean diffierences from those studies providing the Female Sexual Function Index (FSFI) suggested that women with overt hyperthyroidism were associated with a significantly lower FSFI value in FSFI total scores, subscale sexual arousal, lubrication, orgasm, and satisfaction domain (all P < .05). The overall quality of evidence in our study was considered to be moderate. CLINICAL IMPLICATIONS: Clinicians should know the detrimental effects of overt hyperthyroidism on sexual functioning in clinical practice. Measurement of thyroid hormones should be included in the assessment of patients presenting with SD when they show symptoms of clinical hyperthyroidism. STRENGTHS & LIMITATIONS: This is the first meta-analysis quantifying the relationship between overt hyperthyroidism and the risks of SD. However, the combined results were derived from limited retrospective studies along with substantial heterogeneities. CONCLUSION: Our study has confirmed the potentially devastating sexual health consequences caused by overt hyperthyroidism. However, additional rigorous studies with sizable samples are still needed to better elucidate this evidence. Pan Y, Xie Q, Zhang Z, et al. Association Between Overt Hyperthyroidism and Risk of Sexual Dysfunction in Both Sexes: A Systematic Review and Meta-Analysis. J Sex Med 2020;17:2198-2207.

Volunteering and health benefits in general adults: cumulative effects and forms.

BACKGROUND: Although the health benefits of volunteering have been well documented, no research has examined its cumulative effects according to other-oriented and self-oriented volunteering on multiple health outcomes in the general adult public. This study examined other-oriented and self-oriented volunteering in cumulative contribution to health outcomes (mental and physical health, life satisfaction, social well-being and depression). METHODS: Data were drawn from the Survey of Texas Adults 2004, which contains a statewide population-based sample of adults (n = 1504). Multivariate linear regression and Wald test of parameters equivalence constraint were used to test the relationships. RESULTS: Both forms of volunteering were significantly related to better health outcomes (odds ratios = 3.66% to 11.11%), except the effect of self-oriented volunteering on depression. Other-oriented volunteering was found to have better health benefits than did self-volunteering. CONCLUSION: Volunteering should be promoted by public health, education and policy practitioners as a kind of healthy lifestyle, especially for the social subgroups of elders, ethnic minorities, those with little education, single people, and unemployed people, who generally have poorer health and less participation in volunteering.

Occupational segregation and earnings inequality: Rural migrants and local workers in urban China.

This article examines the central role of occupation as the "reward packages" in creating earnings disparities between rural migrants and local workers in urban China's labor markets. Analyses of data from the population mini-census of China in 2005 show that, rural migrants' earnings disadvantages are largely attributable to occupational segregation (between-occupation variation) by workers' household registration status (hukou) rather than unequal pay within the same occupations, but surprisingly they enjoy a slight earnings advantage in lower-status occupations (within-occupation variation). Even after controlling for education and other characteristics, occupational segregation by hukou status continues to exist. The occupational segregation is the most severe in government agencies/state institutions and the least severe in the private sector, leading to earnings disparities between rural migrants and urban local workers in different work unit sectors. Our findings shed new light on how government discriminatory policies could affect occupational segregation and thereby create inequality among social groups in urban China.

Population migration and children's school enrollments in China, 1990-2005.

This paper examines the impact of migration on children's school enrollment by analyzing the micro-data from Chinese population censuses in 1990 and 2000 and mini-census in 2005. We match school-age children (7-14 years old) with their parents, and examine how migration status and parents' absence affect children's school enrollment in urban China. We also compare rural-urban migrant children with their peers in both origin counties and destination districts. Results show that migrant children are less likely to be enrolled in school than urban local children and that children of rural registration status are particularly disadvantaged in school enrollment over the whole examined period in urban China. Rural-urban migrant children fare significantly worse than non-migrant children in both origins and destinations and noticeably they are even less likely than left-behind children to be enrolled in school. The likelihood of being enrolled in school increases for rural-urban migrant children as they spend more time in destinations.

Where There's a Will, There's a Way? Social and Mental Forces of Successful Adaptation of Immigrant Children in Young Adulthood.

Although the twenty-first century is deemed as a new era of globalization, waves of immigration continue, due to disparities between politically and economically unstable regions and Western democratized and developed countries. Immigration research has therefore reignited its attention on the successful adaptation of immigrants' offspring, which has profound implications for Western immigrant-receiving countries, as well as worldwide stability. Although immigration research mainly informed by the conventional assimilation theory and/or segmented assimilation perspective accentuates the importance of structural factors, termed as social forces here, in relation to immigrant children's successful adaptation in adolescence, an argument of determinism and tenability keeps on and the contribution of human mental resources and determination, termed as mental forces here, in shaping life trajectories of immigrant children should be not ignored. For this, with a representative sample of 3344 immigrant children from the Children of Immigrants Longitudinal Study (CILS), we examined and compared both the effects of social and mental forces measured in adolescence of immigrant children on their multiple adaptation outcomes in terms of college graduation, engagement in postgraduate study, and first and current job attainments in young adulthood with a Bayesian multilevel modeling framework. The results found that both social forces of segmented assimilation theory and mental forces of immigrant children in adolescence were significantly predictive of immigrant children's successful adaptation in young adulthood (OR = 1.088-2.959 and ß = 0.050-0.639 for social forces; OR = 11.290-18.119 and ß = 0.293-0.297 for mental forces), in which, although the latter showed stronger effects than the former, the effects of mental forces on adaptation of immigrant children were conditionally shaped by the contexts of the social forces informed by segmented assimilation theory. The findings of the current study highlight the significance of the organism-environment interaction perspective on immigration research and provide an insight to consider a context-driven response thesis proposed.

Exodus from Hunger: The Long-Term Health Consequences of the 1959-1961 Chinese Famine.

This article examines the long-term health consequences of China's 1959-1961 famine by comparing people who stayed in Guangdong and endured the famine with people who crossed the border to immigrate to Hong Kong and thus escaped the famine. Based on data from the Hong Kong Panel Study of Social Dynamics (HKPSSD) and the China Family Panel Studies (CFPS), we focused on two health indicators-body mass index (BMI) and self-rated health (SRH)-of the cohort born before 1959. Our results show that the stayers who experienced the famine have a lower BMI than the emigrants, and they are likely to have a poor SRH. The difference-in-differences (DID) estimates further show that the famine exposure reduced the odds of giving higher ratings of SRH by 60 and 42 percent, respectively, for the 1923-1940 and 1941-1958 birth cohorts. For the 1923-1940 cohort, famine exposure also reduced their BMI by 1.5 points.

Impact of polysomy 17 on HER2 testing of invasive breast cancer patients.

BACKGROUND: Current American Society of Clinical Oncology/College of American Pathologists guidelines define HER2-positive tumors as those with >6 HER2 genes per nucleus or those with HER2/CEP17 (chromosome 17) ratio>2.2. These guidelines are potentially contradictory in tumors with polysomy of chromosome 17. The current study was performed to determine the impact of polysomy 17 on the interpretation of HER2 testing of invasive breast carcinomas. METHODS: Chromosome 17 copies and HER2 gene status were identified by fluorescent in situ hybridization in 384 cases with invasive breast cancer, and the corresponding HER2 expression was obtained by immunohistochemistry stain. RESULTS: The average CEP17 copy number for the group was 2.1 (range, 1.0-12.4). Forty-eight cases (13.8%, 48/348) were identified as chromosome 17 polysomy with CEP 17 copy number≥3. Ninety-two (26.4%) cases had >6 copies of HER2 per nucleus, and 92 cases (26.4%) qualified as HER2 gene amplified using the HER2/CEP17 ratio (>2.2) guideline. Polysomy 17 showed poorly positive correlations with both HER2 gene copy number and HER2 overexpression (P<0.01, r=0.338 and 0.271, respectively). The distribution of clinicopathologic parameters of Polysomy 17 tumors was more similar to HER2 negative than HER2 positive tumors. CONCLUSIONS: Polysomy 17 is a crucial cause of equivocal HER2 testing results by FISH, depending on which criterion (ratio vs. absolute number) is used for interpretation. Polysomy 17 cannot be an independent predictive factor for HER2 gene amplification or protein overexpression.

Social origins, hukou conversion, and the wellbeing of urban residents in contemporary China.

This paper examines the determinants and consequences of rural to urban registration (hukou) mobility in contemporary China, focusing on the link between social origins, hukou conversion, and the consequences of conversion. In contrast to massive rural-urban migration, hukou conversion is difficult and rare, but childhood urban residence increases the likelihood of achieving an urban hukou. Compared to people with urban origins, hukou converters are more likely to enter tertiary institutions, but are significantly less likely to do so subsequent to hukou conversion. Individual converters from rural origins are more likely to work in non-manual occupations. Collective converters earn significantly less than people with urban origins. Individual converters with rural childhoods, who used to be at the bottom of the society, are significantly happier than other urban residents, although they are less healthy. These results show that the effect of hukou conversion varies across hukou converters from different social origins.