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ConspectusAs a renewable solar energy and carbon carrier, biomass exploration has received global attention. Photocatalytic valorization of biomass into fuels and chemicals is a promising and sustainable method for future chemical production. Photocatalysis has the potential to accomplish reactions under ambient conditions due to the unique reaction mechanisms involving photoinduced charge carriers and has recently been recognized as an efficient and feasible technology for biomass conversion. Biomass is widely used as sacrificial agent to scavenge holes in photocatalytic hydrogen evolution, and the carbon is eventually degraded to CO2 with a minor amount of CO. The generation of CO instead of CO2 is more economical and promising but also a challenge under photoreforming conditions.This is a new research direction, while until now there has still been the lack of a comprehensive review article to summarize and provide prospects for this topic. This Account will highlight our contributions in the research direction of the photocatalytic reforming of biomass into syngas (CO + H2). In 2020, we first reported the photocatalytic conversion of biopolyols and sugars into syngas by employing a defect-rich Cu-TiO2 nanorod photocatalyst and found that formic acid is a key intermediate to CO. Further study revealed that a facet-dependent electron-trapping state on anatase TiO2 will affect the photocatalytic dehydration activity for formic acid intermediates by regulating the electron transfer process during the reaction, and the selective generation of FA or CO from photocatalytic biomass reforming was achieved via exposing the (100) or (101) facets, respectively. Visible light-driven syngas generation was further achieved over a CdS-based photocatalyst. Sulfate modification of CdS ([SO4]/CdS) was constructed as the proton acceptor, thus efficiently facilitating the proton-coupled electron transfer process. Besides, we put forward an oxygen-controlled strategy to increase the CO generation rate without a significant decrease in CO selectivity via controlling the O2/substrate ratio. Based on this system, a Z-scheme CdS@g-C3N4 core-shell structure and CdO-CdS semicoherent interface were created to facilitate charge transfer and enhance the O2 activation, thus increasing the CO generation rate. Moreover, we also developed a photoelectrochemical approach to separately produce CO and H2 from biomass. Nitrogen doping of a hexagonal WO3 nanowire array was used to produce the photoanode. The built-in electric field generated via nitrogen doping promoted charge transfer, hence improving the efficiency of PEC reforming of biopolyols and sugars. This Account will systematically analyze the challenges in this research direction, the reaction route in the photocatalytic biomass reforming, and the factors affecting CO selectivity and give insight into the design of efficient photocatalytic systems.
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BACKGROUND: Injecting botulinum toxin (BTX) into the submandibular glands (SMGs) can treat drooling symptoms in neurological diseases and improve the aesthetics of SMG hypertrophy and ptotic SMGs. OBJECTIVES: This study aimed to define the size and position of the SMGs by high-frequency ultrasound, and to perform statistical analysis to improve the safety and accuracy of BTX injection therapy. METHODS: Neck ultrasonography with high-frequency ultrasound was performed on 214 volunteers. The length, height, and thickness of the SMGs, and the distance between the SMGs and the midline, the anterior border of the sternocleidomastoid, the mandible, and the surface were measured. RESULTS: The SMGs were almond-shaped with a mean [standard deviation] length of 33.7 [4.7]â mm, a thickness of 13.3 [2.9]â mm, and a height of 27.6 [6.0]â mm. The length and height were significantly different between underage and youth groups. The size of the SMGs did not show any notable differences with increasing BMI; however, their depth, and the distance from the mandible, midline, and anterior border of the sternocleidomastoid increased. No significant differences were observed between the affected and healthy sides in patients with microtia, hemifacial microsomia, or cleft lip and palate. CONCLUSIONS: Ultrasound provides more comprehensive information regarding the size and position of the SMGs, which can serve as a reference in BTX therapy and in the diagnosis of SMG diseases involving size alterations.
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Glândula Submandibular , Ultrassonografia , Humanos , Feminino , Masculino , Glândula Submandibular/diagnóstico por imagem , Adulto , Ultrassonografia/métodos , Adulto Jovem , Adolescente , Pessoa de Meia-Idade , Criança , Sialorreia/etiologia , Sialorreia/diagnóstico por imagemRESUMO
The electron transfer (ET) from the conduction band of the semiconductor to surface-bound species is a key step in the photocatalytic reaction and strongly affects the reactivity and selectivity, while the effect of catalyst surface structure on this process has rarely been explored due to the lack of an effective method. Herein, we have developed a strategy to detect and measure surface electrons' transfer energy to the adsorbates and disclosed a facet-dependent electron transfer energy over anatase TiO2. The photogenerated electrons are shallowly confined in the five-coordinated Ti atom (Ti5c) on the surface of the (101) facet with a transfer energy below 1.0 eV, while deeply confined in the six-coordinated Ti atom (Ti6c) on the subsurface of the (001) facet with a transfer energy higher than 1.9 eV. The different electron trap states strongly affect the ET process, thus regulating the photocatalytic activity. Taking formic acid (FA) dehydration as the probe reaction, a shallow trap of photoexcited electrons on the (101) facet of anatase TiO2 favors the dehydration of FA to CO, while a deep trap of photoexcited electrons on the (001) facet makes FA stable. Based on this knowledge, we successfully controlled the selectivity in the photocatalytic oxidation of biopolyols via selectively exposing the facet of TiO2. Through controlling the (001)/(101) facet, a wide range of biopolyols can be selectively converted into FA or CO with a selectivity of up to 80%. The present work disclosed a facet-dependent electron transfer process and provides a new horizon to the design of photocatalytic systems.
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Elétrons , Processos Fotoquímicos , Humanos , Desidratação , Titânio/químicaRESUMO
CO and H2 evolution from renewable and abundant biomass represent a sustainable way, but is challenged to be produced under mild conditions. Herein, we propose to produce CO and H2 from biomass via a divided photoelectrochemical (PEC) cell at room temperature. Nitrogen doped tungsten trioxide (N-WO3 ) photoanode reforms biopolyols to CO and H+ , and platinum cathode reduces H+ to H2 , achieving CO evolution rate of 45â mmol m-2 h-1 (>75 % gas selectivity) and H2 evolution rate of 237â mmol m-2 h-1 with purity >99.99 % from glycerol. The nitrogen doping induces structure polarity of WO3 photoanode, leading to the formation of an internal electric field which promotes the separation and transfer of the photoinduced charges and improves PEC efficiency. A wide range of biopolyols, such as ethylene glycol, xylose, fructose, glucose, sucrose, lactose, maltose, and inulin were effectively converted into CO and H2 . This work provides a promising method to produce highly pure H2 together with CO from biomass.
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Nitrogênio , Platina , Biomassa , Etilenoglicóis , Frutose , Glucose , Glicerol , Inulina , Lactose , Maltose , Polímeros , Sacarose , XiloseRESUMO
Photocatalytic biomass conversion represents an ideal way of generating syngas because of the sustainable use of biomass carbon and solar energy. However, the lack of efficient electron-proton transfer limits its efficiency. We here report an unprecedented method to simultaneously increase both the electron and proton transfer by creating surface sulfate ions on the CdS catalyst ([SO4]/CdS). Surface sulfate ion [SO4] is bifunctional, serving as the proton acceptor to promote proton transfer, and increasing the oxidation potential of the valence band to enhance electron transfer. [SO4]/CdS produces a syngas mixture from glycerol without CO2. Compared with pristine CdS, [SO4]/CdS exhibits 9-fold higher CO generation rate (0.31 mmol g-1 h-1) and 4-fold higher H2 generation (0.05 mmol g-1 h-1). A wide range of sugars, such as glucose, fructose, maltose, sucrose, xylose, lactose, insulin, and starch, were facilely converted into syngas. This study reports the pivotal effect of surface sulfate ion on electron-proton transfer in photocatalysis and provides a facile method for increasing photocatalytic efficiency.
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Biocombustíveis , Compostos de Cádmio/química , Dissacarídeos/química , Glicerol/química , Monossacarídeos/química , Polímeros/química , Sulfetos/química , Biomassa , Catálise , Nanotubos/química , Sulfatos/químicaRESUMO
Mesoporous metal sulfides (MMSs) with high surface areas and large pore volumes show great potential in many applications such as gas sensing, photodetection, and catalysis. However, the synthesis of MMSs is still challenging due to the uncontrollable fast precipitation between metal ions and S2- ions and the large volume contraction during the conversion of metal precursors to sulfides. Here, a general polymer-oriented acid-mediated self-assembly method to synthesize highly crystalline MMSs (e.g., ZnS, CdS, Ni3 S4 , CuS, and Znx Cd1- x S) by using polyethylenimine (PEI) as pore-forming agent is reported. In this method, acetic acid is designed as pH regulator and coordination agent to control the interactions between inorganic precursors and PEI, and adjust the reaction kinetics of metal ions and thioacetamide. This method endows a high degree of control over crystal structure and porous structure of MMSs. The surface areas and pore volumes of obtained MMSs are as high as 157 m2 g-1 and 1.149 cm3 g-1 , respectively. Benefiting from the abundant mesopores and homojunctions, mesoporous Zn0.56 Cd0.44 S shows a superior photocatalytic H2 generation rate of 14.3 mmol h-1 g-1 .
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Producing renewable biofuels from biomass is a promising way to meet future energy demand. Here, we demonstrated a lignin to diesel route via dimerization of the lignin oil followed by hydrodeoxygenation. The lignin oil undergoes C-C bond dehydrogenative coupling over Au/CdS photocatalyst under visible light irradiation, co-generating diesel precursors and hydrogen. The Au nanoparticles loaded on CdS can effectively restrain the recombination of photogenerated electrons and holes, thus improving the efficiency of the dimerization reaction. About 2.4â mmol gcatal-1 h-1 dimers and 1.6â mmol gcatal-1 h-1 H2 were generated over Au/CdS, which is about 12 and 6.5 times over CdS, respectively. The diesel precursors are finally converted into C16-C18 cycloalkanes or aromatics via hydrodeoxygenation reaction using Pd/C or porous CoMoS catalyst, respectively. The conversion of pine sawdust to diesel was performed to demonstrate the feasibility of the lignin-to-diesel route.
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Mesoporous metal oxides (MMOs) have been demonstrated great potential in various applications. Up to now, the direct synthesis of MMOs is still limited to the solvent induced inorganic-organic self-assembly process. Here, we develop a facile, general, and high throughput solvent-free self-assembly strategy to synthesize a series of MMOs including single-component MMOs and multi-component MMOs (e.g., doped MMOs, composite MMOs, and polymetallic oxide) with high crystallinity and remarkable porous properties by grinding and heating raw materials. Compared with the traditional solution self-assembly process, the avoidance of solvents in this method not only greatly increases the yield of target products and synthesis efficiency, but also reduces the environmental pollution and the consumption of cost and energy. We believe the presented approach will pave a new avenue for scalable production of advanced mesoporous materials for various applications.
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In this study, a two-dimensional heterojunction consisting of bismuth trioxide nanoplates and layered reduced graphene oxide was synthesized using a facile In Situ growth route. A series of characterization tests indicated that the reduction of graphene oxide played a key role as an electron collector for enhancing photoinduced charge carrier separation efficiency. Thus, the as-prepared reduced graphene oxide/bismuth trioxide composite exhibited enhanced photocatalytic water oxidation, which was higher than that of pristine bismuth trioxide under simulated solar light irradiation. Furthermore, the photoelectrochemical properties of the prepared hybrid system were investigated to understand the transfer of photoinduced electrons and holes between layered reduced graphene oxide and bismuth trioxide nanoplates. Thus, this strategy provided an efficient approach for the fabrication of graphene composites containing hierarchical ternary oxides for photocatalysis.
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Mutations in the RNA-binding protein FUS/TLS (FUS) have been linked to the neurodegenerative disease amyotrophic lateral sclerosis (ALS). Although predominantly nuclear, this heterogenous nuclear ribonuclear protein (hnRNP) has multiple functions in RNA processing including intracellular trafficking. In ALS, mutant or wild-type (WT) FUS can form neuronal cytoplasmic inclusions. Asymmetric arginine methylation of FUS by the class 1 arginine methyltransferase, protein arginine methyltransferase 1 (PRMT1), regulates nucleocytoplasmic shuttling of FUS. In motor neurons of primary spinal cord cultures, redistribution of endogenous mouse and that of ectopically expressed WT or mutant human FUS to the cytoplasm led to nuclear depletion of PRMT1, abrogating methylation of its nuclear substrates. Specifically, hypomethylation of arginine 3 of histone 4 resulted in decreased acetylation of lysine 9/14 of histone 3 and transcriptional repression. Distribution of neuronal PRMT1 coincident with FUS also was detected in vivo in the spinal cord of FUS(R495X) transgenic mice. However, nuclear PRMT1 was not stable postmortem obviating meaningful evaluation of ALS autopsy cases. This study provides evidence for loss of PRMT1 function as a consequence of cytoplasmic accumulation of FUS in the pathogenesis of ALS, including changes in the histone code regulating gene transcription.
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Esclerose Lateral Amiotrófica/metabolismo , Citoplasma/metabolismo , Proteína-Arginina N-Metiltransferases/metabolismo , Proteína FUS de Ligação a RNA/metabolismo , Proteínas Repressoras/metabolismo , Esclerose Lateral Amiotrófica/patologia , Animais , Núcleo Celular/metabolismo , Células Cultivadas , Metilação de DNA , Modelos Animais de Doenças , Histonas/metabolismo , Humanos , Camundongos , Camundongos Transgênicos , Neurônios Motores/metabolismo , Medula Espinal/metabolismoRESUMO
Photocatalysis has shown great potential in organic reactions, while controlling the selectivity is a long-standing goal and challenge due to the involvement of various radical intermediates. In this study, we have realized selectivity control in the photocatalytic conversion of alcohols via engineering the surface static electric field of the CdS semiconductor. By leveraging the Au-CdS interaction to adjust lattice strain, which influences the intensity of the surface static electric field, we altered the pathways of alcohol conversion. The increased intensity of the surface static electric field changed the activation pathways of the C-H/O-H bond, leading to the selective formation of targeted C/O-based radical intermediates and altering the selectivity from aldehydes to dimers. A wide range of alcohols, such as aromatic alcohol and thiophenol alcohol, were selectively converted into aldehyde or dimer. This work provides an effective strategy for selectively controlling reaction pathways by generating a surface electric field.
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BACKGROUND: Postburn axillary contracture is a common complication that leads to functional impairment and unsatisfactory aesthetic outcomes. This article aims to present our experience with axillary contracture reconstruction using pre-expanded brachial artery perforator propeller (BAPP) flaps and provide a systematic review of other regional or free flaps. METHODS: This retrospective study included patients who underwent postburn axillary contracture reconstruction using pre-expanded BAPP flaps from 2015 to 2022. Data on the flap characteristics and function of the affected shoulders were recorded. A systematic review was conducted by retrieving studies that assessed the outcomes of regional or free cutaneous/fasciocutaneous flaps for treating axillary contracture from PubMed, Web of Science, EMBASE, and Scopus published before October 1, 2023. RESULTS: Twelve pre-expanded BAPP flaps measuring up to 26 cm × 11 cm (mean, 116.9 cm 2) survived completely with no major complications, and the donor sites were closed primarily. The average range of shoulder abduction increased from 77.9° to 141.7° (p=0.002). The systematic review included 34 articles, reporting 12 regional and three free flaps. The most reported flaps were the thoracodorsal artery perforator flap, scapular flap, and parascapular flap. The overall complication rate ranged from 0 to 25%, and the average change in shoulder abduction ranged from 72.5° to 99.4°. CONCLUSIONS: Pre-expanded BAPP flaps can be effectively used for reconstructing postburn anterior axillary fold contracture. The donor site availability and the specific axillary contracture type should be considered when selecting a regional or free flap.
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Selective C-O bond cleavage is an efficient way for the biomass valorization to value-added chemicals, but is challenged to be operated at room temperature via conventional thermal catalysis. Herein, inspired from the DNA biosynthesis which involves a radical-mediated spin-center shift (SCS) C-O bond cleavage process, we report a biomimetic room-temperature C-O bond cleavage of vicinal diol (HOCHCH-OH). We construct a Mn doped CdS (Mn/CdS) as a photocatalyst to mimic the biologic SCS process. The Mn site plays pivotal role: (1) accelerates the photo-induced carrier separation, promoting the hole-mediated C-H bond cleavage to generate carbon-centered radicals, and (2) serves as the binding site for -OH groups, making it to be an easier leaving group. Mn/CdS achieves 0.28â mmol gcat -1 h-1 of hydroxyacetone (HA) from glycerol dehydration at room temperature under visible light irradiation, which is 3.5-fold that over pristine CdS and 40-fold that over bulk MnS/CdS. This study provides a new biomimetic room-temperature C-O bond cleavage process, which is promising for the biomass valorization.
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Photocatalytic oxidation shows great potential in the valorization of biomass under mild conditions, while the selectivity control is particularly challenging for the complex and reactive bio-polyols. Herein, we report a selective photocatalytic process to convert bio-polyols into formic acid (FA) or carbon monoxide (CO) by controlling the phase of TiO2. The bio-polyols are facially oxidized to formic acid (FA) which is stable over rutile and could be dehydrated to CO over anatase TiO2. Through controlling the phase, FA or CO could be obtained from a wide range of bio-polyols with selectivity up to 63% or 52%. Our studies elucidate that the phase-dependent selectivity is essentially derived from the difference in the adsorption configuration of FA. In situ Fourier transform infrared spectroscopy (FTIR) and density functional (DFT) calculations were used to study the FA decomposition process on the surface of TiO2. The phase-dependent FA decomposition is mainly derived from the different surface geometry, which affects the configuration of FA adsorption. Molecular adsorbed FA on anatase favors the dehydration of FA to CO while bidentate dissociated adsorption of FA on the rutile phase is inert to be further converted. This work provides a new horizon to the design of photocatalytic systems for biomass conversion.
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Switchable catalysis promises exceptional efficiency in synthesizing polymers with ever-increasing structural complexity. However, current achievements in such attempts are limited to constructing linear block copolymers. Here we report a visible light regulated switchable catalytic system capable of synthesizing hyperbranched polymers in a one-pot/two-stage procedure with commercial glycidyl acrylate (GA) as a heterofunctional monomer. Using (salen)CoIIICl (1) as the catalyst, the ring-opening reaction under a carbon monoxide atmosphere occurs with high regioselectivity (>99% at the methylene position), providing an alkoxycarbonyl cobalt acrylate intermediate (2a) during the first stage. Upon exposure to light, the reaction enters the second stage, wherein 2a serves as a polymerizable initiator for organometallic-mediated radical self-condensing vinyl polymerization (OMR-SCVP). Given the organocobalt chain-end functionality of the resulting hyperbranched poly(glycidyl acrylate) (hb-PGA), a further chain extension process gives access to a core-shell copolymer with brush-on-hyperbranched arm architecture. Notably, the post-modification with 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) affords a metal-free hb-PGA that simultaneously improves the toughness and glass transition temperature of epoxy thermosets, while maintaining their storage modulus.
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Mutations in the RNA-binding protein FUS (fused in sarcoma) are linked to amyotrophic lateral sclerosis (ALS), but the mechanism by which these mutants cause motor neuron degeneration is not known. We report a novel ALS truncation mutant (R495X) that leads to a relatively severe ALS clinical phenotype compared with FUS missense mutations. Expression of R495X FUS, which abrogates a putative nuclear localization signal at the C-terminus of FUS, in HEK-293 cells and in the zebrafish spinal cord caused a striking cytoplasmic accumulation of the protein to a greater extent than that observed for recessive (H517Q) and dominant (R521G) missense mutants. Furthermore, in response to oxidative stress or heat shock conditions in cultures and in vivo, the ALS-linked FUS mutants, but not wild-type FUS, assembled into perinuclear stress granules in proportion to their cytoplasmic expression levels. These findings demonstrate a potential link between FUS mutations and cellular pathways involved in stress responses that may be relevant to altered motor neuron homeostasis in ALS.
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Esclerose Lateral Amiotrófica/genética , Proteína FUS de Ligação a RNA/fisiologia , Adulto , Animais , Linhagem Celular , Citoplasma/metabolismo , Feminino , Proteínas de Fluorescência Verde/genética , Humanos , Masculino , Pessoa de Meia-Idade , Mutação de Sentido Incorreto , Estresse Oxidativo , Proteína FUS de Ligação a RNA/genética , Proteína FUS de Ligação a RNA/metabolismo , Peixe-ZebraRESUMO
Bronchogenic cysts are congenital malformations caused by aberrant foregut budding. They major occur in the thorax, with subdiaphragmatic cases being uncommon. Here, we present a series of 19 patients diagnosed with subdiaphragmatic bronchogenic cysts histopathologically at a single institution in China from 2012 to 2021. A literature review was also conducted by searching the PubMed database using keywords related to "bronchogenic cysts" and "subdiaphragmatic," yielding 107 cases. Taken together, the 126 cases had a median age of 41.0 years (interquartile range, 30.0-51.0 years) and 62 of them were male (49.2%). The cysts were most commonly detected in the left adrenal region (36.2%), followed by the pancreatic region (11.5%) and gastric cardia/lesser curvature of the stomach (9.2%). All patients except two underwent surgery for a definite diagnosis, symptom alleviation, and (or) malignancy prevention. Most patients recovered fast and were discharged from the hospital within 1 week after surgery, and the surgical complications were infrequent. The prognosis was generally favorable, as no recurrence was reported during the follow-up as long as 77 months.
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Recent studies indicate existence of beige adipocytes in adults. Upon activation, beige adipocytes burn energy for thermogenesis and contribute to regulation of energy balance. In this study, we have analyzed whether Jinlida granules (JLD) could activate beige adipocytes. JLD suspended in 0.5% carboxymethyl cellulose (CMC) was gavage fed to db/db mice at a daily dose of 3.8 g/kg. After 10 weeks, body weight, biochemical, and histological analyses were performed. In situ hybridization, immunofluorescence, and western blotting were conducted to test beige adipocyte activation in mice. X9 cells were induced with induction medium and maintenance medium containing 400 µg/mL of JLD. After completion of induction, cells were analyzed by Nile red staining, time polymerase chain reaction (PCR), western blotting, and immunofluorescence to understand the effect of JLD on the activation of beige adipocytes. A molecular docking method was used to preliminarily identify compounds in JLD, which hold the potential activation effect on uncoupling protein 1 (UCP1). JLD treatment significantly improved obesity in db/db mice. Biochemical results showed that JLD reduced blood glucose (GLU), triglyceride (TG), and low-density lipoprotein cholesterol (LDL) levels as well as liver aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels in mice. Hematoxylin and eosin staining (H&E) showed that JLD reduced hepatocyte ballooning changes in the liver. Immunofluorescence showed that JLD increased the expression of the thermogenic protein, UCP1, in the beige adipose tissue of mice. JLD also increased the expression of UCP1 and inhibited the expression of miR-27a in X9 cells. Molecular docking results showed that epmedin B, epmedin C, icariin, puerarin, and salvianolic acid B had potential activation effects on UCP1. The results suggest that JLD may activate beige adipocytes by inhibiting miR-27a expression, thereby promoting thermogenesis in beige adipocytes. This study provides a new pharmacological basis for the clinical use of JLD.
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Adipócitos Bege , MicroRNAs , Adipócitos Bege/metabolismo , Animais , Medicamentos de Ervas Chinesas , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos , MicroRNAs/metabolismo , Simulação de Acoplamento Molecular , Obesidade/tratamento farmacológico , Obesidade/metabolismo , Proteína Desacopladora 1/genética , Proteína Desacopladora 1/metabolismoRESUMO
Hyperkalemic periodic paralysis (HyperKPP) produces myotonia and attacks of muscle weakness triggered by rest after exercise or by K+ ingestion. We introduced a missense substitution corresponding to a human familial HyperKPP mutation (Met1592Val) into the mouse gene encoding the skeletal muscle voltage-gated Na+ channel NaV1.4. Mice heterozygous for this mutation exhibited prominent myotonia at rest and muscle fiber-type switching to a more oxidative phenotype compared with controls. Isolated mutant extensor digitorum longus muscles were abnormally sensitive to the Na+/K+ pump inhibitor ouabain and exhibited age-dependent changes, including delayed relaxation and altered generation of tetanic force. Moreover, rapid and sustained weakness of isolated mutant muscles was induced when the extracellular K+ concentration was increased from 4 mM to 10 mM, a level observed in the muscle interstitium of humans during exercise. Mutant muscle recovered from stimulation-induced fatigue more slowly than did control muscle, and the extent of recovery was decreased in the presence of high extracellular K+ levels. These findings demonstrate that expression of the Met1592ValNa+ channel in mouse muscle is sufficient to produce important features of HyperKPP, including myotonia, K+-sensitive paralysis, and susceptibility to delayed weakness during recovery from fatigue.
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Músculo Esquelético/metabolismo , Miotonia/metabolismo , Miotonia/patologia , Potássio/metabolismo , Canais de Sódio/metabolismo , Envelhecimento/fisiologia , Animais , Progressão da Doença , Eletrofisiologia , Regulação da Expressão Gênica , Humanos , Camundongos , Camundongos Transgênicos , Mutação/genética , Miotonia/genética , Oxirredução , Paralisia Periódica Hiperpotassêmica/genética , Paralisia Periódica Hiperpotassêmica/metabolismo , Paralisia Periódica Hiperpotassêmica/patologia , Fenótipo , RNA Mensageiro/genética , Sensibilidade e Especificidade , Canais de Sódio/genéticaRESUMO
Patient-reported outcome measures (PROMs) are being used increasingly in total knee arthroplasty (TKA). We conducted a systematic review aimed at identifying psychometrically sound PROMs by appraising their measurement properties. Studies concerning the development and/or evaluation of the measurement properties of PROMs used in a TKA population were systematically retrieved via PubMed, Web of Science, Embase, and Scopus. Ratings for methodological quality and measurement properties were conducted according to updated COnsensus-based Standards for the selection of health Measurement INstruments (COSMIN) methodology. Of the 155 articles on 34 instruments included, nine PROMs met the minimum requirements for psychometric validation and can be recommended to use as measures of TKA outcome: Oxford Knee Score (OKS); OKS-Activity and Participation Questionnaire (OKS-APQ); 12-item short form Knee Injury and Osteoarthritis Outcome (KOOS-12); KOOS Physical function Short form (KOOS-PS); Western Ontario and McMaster Universities Arthritis Index-Total Knee Replacement function short form (WOMAC-TKR); Lower Extremity Functional Scale (LEFS); Forgotten Joint Score (FJS); Patient's Knee Implant Performance (PKIP); and University of California Los Angeles (UCLA) activity score. The pain and function subscales in WOMAC, as well as the pain, function, and quality of life subscales in KOOS, were validated psychometrically as standalone subscales instead of as whole instruments. However, none of the included PROMs have been validated for all measurement properties. Thus, further studies are still warranted to evaluate those PROMs. Use of the other 25 scales and subscales should be tempered until further studies validate their measurement properties. Cite this article: Bone Joint Res 2021;10(3):203-217.