The role of stimulator of interferon genes-mediated AMPK/mTOR/P70S6K autophagy pathway in cyfluthrin-induced testicular injury.

Cyfluthrin is widely used in the field of sanitary pest control by its wide insecticidal spectrum, high efficiency and low toxicity, low residue, and good biodegradability. But, as a double-edged sword, a large amount of cyfluthrin remains are still in the environment. The residual cyfluthrin is absorbed into the food chain through vegetation and then poses a risk to soil organisms and human health. Several studies have suggested that cyfluthrin is one of the main factors causing testicular damage, but the mechanism remains unclear. In this study, we established in vivo and in vitro models of testicular injury in rats and GC-2 cells exposed to cyfluthrin to explore whether stimulator of interferon genes (STING) gene mediates the regulation of AMPK/mTOR/p70S6K autophagy pathway, which lays a foundation for further study of the mechanism of testicular injury induced by cyfluthrin. The results showed that the activity of super oxide dismutase in testis decreased and the activity of malonic dialdehyde increased with the increase of concentration in vivo and in vitro. At the same time, the levels of mitochondrial damage and inflammation in the testis also increased, which further activated autophagy. In this process, the increased level of inflammation is related to the increased expression of STING gene, and AMPK/mTOR/p70S6K autophagy pathway is also involved. To sum up, cyfluthrin has certain reproductive toxicity, and long-term exposure can induce testicular cell damage. STING gene can participate in cyfluthrin-induced testicular injury through AMPK/mTOR/P70S6K autophagy pathway.

MAPK pathway mediates epithelial-mesenchymal transition induced by paraquat in alveolar epithelial cells.

Epithelial-mesenchymal transition (EMT) is believed to be involved in lung fibrosis process induced by paraquat (PQ); however, the molecular mechanism of this process has not been clearly established. The present study investigated the potential involvement of EMT after PQ poisoning. The expressions of EMT markers, such as E-cadherin and α-smooth muscle actin (α-SMA), at multiple time points after exposure to different concentrations of PQ were evaluated by western blot analysis. Following PQ treatment, EMT induction was observed under microscopy. Related fibrosis genes, including Matrix metalloproteinase 2 (MMP-2), Matrix metalloproteinase 9 (MMP-9), collagens type I (COL I), and type III (COL III), were also evaluated by measuring their mRNA levels using RT-PCR analysis. Signaling pathways were analyzed using selective pharmacological inhibitors for MAPK. Cell migration ability was evaluated by scratch wound and Transwell assays. The data showed that PQ-induced epithelial RLE-6NT cells to develop mesenchymal cell characteristics, as indicated by a significant decrease in the epithelial marker E-cadherin and a significant increase in the extracellular matrix (ECM) marker α-smooth muscle actin in a dose and time-dependent manner. Moreover, PQ-treated RLE-6NT cells had an EMT-like phenotype with elevated expression of MMP-2, MMP-9, and COL I and COL III and enhanced migration ability. Signal pathway analysis revealed that PQ-induced EMT led to ERK-1 and Smad2 phosphorylation through activation of the MAPK pathway. The results of the current study indicate that PQ-induced pulmonary fibrosis occurs via EMT, which is mediated by the MAPK pathway. This implies that the MAPK pathway is a promising therapeutic target in alveolar epithelial cells. © 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 1407-1414, 2016.

[Relationship between occupational stress and blood glucose, blood lipid, blood pressure of video display terminal operators].

OBJECTIVE: To explore the relationship between occupational stress and blood glucose, Blood lipid and blood pressure. METHODS: 108 video display terminals(VDT) operators who had the working experience were recruited to the study. The occupational stress indicator (OSI), the lever of blood glucose, cholesterol, triglyceride, lipoprotein of high density and lipoprotein of low density in serum were measured by using GOD-PAP, CHOD-PAP, GPO-PAP and PVS. The subjects were classified into three groups according to the score of occupational stress. RESULTS: The contents of blood glucose of low, middle and high level of stress groups were (3.39 +/- 1.24), (3.59 +/- 1.26), (2.54 +/- 0.94) mmol/L respectively (F = 7.324, P < 0.01), and with the increase of level of stress, the content of blood glucose decreased significantly (r = -0.376, P < 0.01). CONCLUSION: The level of blood glucose of VDT operators is affected by occupational stress, among video display terminals and it can be used as the index for estimating occupational stress.

[A study on occupational stress in commercial workers of a supermarket in Yinchuan].

OBJECTIVE: To study difference in occupational stress between men and women commercial workers in a supermarket in Yinchuan, Ningxia. METHODS: Totally, 679 commercial workers in a supermarket were investigated with questionnaire of occupational stress indicator (OSI), matched on age, length of service, educational level, marital status and type of work. RESULTS: Score of occupational stress factors, relationships, home/work balance and organizational atmosphere in women commercial workers was 143.48, 30.86, 20.82 and 15.16, respectively, obviously higher than that in men, with 134.89, 28.61, 18.75 and 13.93, respectively. Score of psychological health and satisfaction in women was 39.86 and 14.82, respectively, lower than that in men, with 43.84 and 17.66, respectively, which indicate that occupational stress in women was more severe with a more stressful psychological reaction than in men. Stepwise regression analysis showed that the main predicting factor for job satisfaction was personal relationships in women, and organizational atmosphere, managerial role and workload in men. Those for psychological health was control strategy and organizational atmosphere in women, and organizational atmosphere and recognition in men, those for physical illness was workload in women and support strategy and physical exercises in men, and those for stress level was support strategy in women and coping strategy in men. CONCLUSIONS: Women commercial workers experienced much more stress, with more severe stress reaction in their work, than men did. The main factors affecting occupational stress reaction and level of stress in women and men were not quite similar.