Restrictive Breathing Mask Reduces Repetitions to Failure During a Session of Lower-Body Resistance Exercise.

Andre, TL, Gann, JJ, Hwang, PS, Ziperman, E, Magnussen, MJ, and Willoughby, DS. Restrictive breathing mask reduces repetitions to failure during a session of lower-body resistance exercise. J Strength Cond Res 32(8): 2103-2108, 2018-The purpose of this study was to determine the effect of restrictive breathing mask (RBM) on muscle performance, hemodynamic, and perceived stress variables during a session of lower-body resistance exercise. In a crossover design, 10 participants performed 2 separate testing sessions, RBM and no mask, consisting of squat, leg press, and leg extension. The paired-samples t-test was used for session rating of perceived exertion (S-RPE), perceived stress before and after, heart rate (HR), pulse oximetry, and a 2 × 4 (session [mask, no mask] × time [squat exercise, leg press exercise, leg extension exercise, total resistance exercise session]) factorial analysis of variance with repeated measures (p ≤ 0.05). A significant decrease was found in total repetitions during the RBM condition (p < 0.01). A majority of the decrease in repetitions to failure occurred in the squat (p < 0.05) and in the leg press (p < 0.01), whereas no difference was observed in leg extension (p = 0.214). A significant increase was observed in S-RPE during the RBM session (p < 0.01). A significant increase was found in prestress (p < 0.01) and poststress (p = 0.01) in the RBM session. No significant difference existed for HR between exercise sessions (p = 0.08). A significant decrease existed in pulse oximetry during the RBM session (p < 0.01). The use of an RBM had a negative effect on the number of repetitions completed during an acute session of lower-body resistance training.

Release of Carbohydrate-Metal Adducts from Electrospray Droplets: Insight into Glycan Ionization by Electrospray.

Glycans have an immense number of biological activities, necessitating increased efforts to characterize glycan structures. Mass spectrometry has been coupled to electrospray ionization (ESI) to characterize carbohydrates. While the gas-phase structures of glycan- and carbohydrate-metal adducts have been characterized, several questions persist concerning the mechanism of transfer of carbohydrates from ESI droplets into the gas phase. Using various computational methods, including molecular dynamics, steered molecular dynamics, and density functional theory calculations, we present a mechanistic investigation on the evaporation of solvent from nanosized droplets, formation of carbohydrate-metal adducts, and their subsequent release into the gas phase. We relate the computational results to mass spectra of melezitose, a model carbohydrate, and its permethylated derivative. Our results confirm two mechanisms for the release of carbohydrate-ion adducts from solvated droplets. Native (unmodified) carbohydrates are ionized via the charged residue model, while the permethylated derivative is ionized via the ion evaporation model. For both mechanisms, the monomer carbohydrate-metal adduct is the dominant species observed. This work illustrates that the ionization mechanisms are dictated by interactions between the carbohydrate and solvent, and coordination of the carbohydrate with the metal ion. Thus, these results provide insight into the molecular interactions that govern the mechanism of release.