The rating of perceived exertion is able to differentiate the post-matches metabolomic profile of elite U-20 soccer players.

PURPOSE: The study aimed to assess the metabolic impact of elite Brazilian U-20 players using the rating of perceived exertion scale (RPE) to discriminate metabolomics sensitivity post-two soccer games separated by a short recovery interval. METHODS: Urine was collected immediately and then 20 h after two soccer matches of elite Brazilian U-20 players. RPE was collected after games. The spectra were pre-processed using TopSpin®3.2 software. Chenomx®software was used to identify metabolites in the urine through the available database. RESULTS: The results showed that the metabolic pathways related to energy production, cellular damage, and organic stresses were changed immediately after the game. 20 h after the games, antioxidant and anti-inflammatory pathways related to cell recovery were identified (e.g., gallic acid, ascorbate, and betaine). The matrix of positive correlations between metabolites was more predominant and stronger after game 2 than game 1. T-distribution registered metabolites discriminated below and above 7 on the RPE scale. Athletes with higher RPE values showed a high metabolite profile related to muscle damage (e.g., creatine, creatinine, and glycine) and energy production (e.g., creatine, formate, pyruvate, 1,3 dihydroxyacetone) 20 h post-soccer match. There was a different metabolic profile between athletes with higher and lower RPE values. CONCLUSION: Metabolomics analysis made it possible to observe the metabolic impacts of energy production and muscular damage. RPE identified internal load changes within the group as a result of match intensity in soccer. The correlation matrix indicated a greater predominance of positive and strong correlations between metabolites in the second game compared to the first game.

Molecular Modeling and Physicochemical Properties of Supramolecular Complexes of Limonene with α- and ß-Cyclodextrins.

This study evaluated three different methods for the formation of an inclusion complex between alpha- and beta-cyclodextrin (α- and ß-CD) and limonene (LIM) with the goal of improving the physicochemical properties of limonene. The study samples were prepared through physical mixing (PM), paste complexation (PC), and slurry complexation (SC) methods in the molar ratio of 1:1 (cyclodextrin:limonene). The complexes prepared were evaluated with thermogravimetry/derivate thermogravimetry, infrared spectroscopy, X-ray diffraction, complexation efficiency through gas chromatography/mass spectrometry analyses, molecular modeling, and nuclear magnetic resonance. The results showed that the physical mixing procedure did not produce complexation, but the paste and slurry methods produced inclusion complexes, which demonstrated interactions outside of the cavity of the CDs. However, the paste obtained with ß-cyclodextrin did not demonstrate complexation in the gas chromatographic technique because, after extraction, most of the limonene was either surface-adsorbed by ß-cyclodextrin or volatilized during the procedure. We conclude that paste complexation and slurry complexation are effective and economic methods to improve the physicochemical character of limonene and could have important applications in pharmacological activities in terms of an increase in solubility.

Assessment of thimerosal effects in sublethal concentrations on zebrafish (Danio rerio) embryos exploring NMR-based metabolomics profile.

Thimerosal, a preservative commonly used in the pharmaceutical and cosmetic industry, has raised concerns regarding its potentially toxic effects as an organic mercury compound. Within this context, using an NMR-based metabolomics profile and chemometric analysis, zebrafish embryos were used as an in vivo model to study the effects of thimerosal in metabolic profiles after exposure to sublethal concentrations of the mercury compound. The thimerosal concentrations of 40 and 80 nM were employed, corresponding to 40% and 80% of the LC50, respectively, for zebrafish embryos. The most significant alterations in the metabolic profile included changes in carbohydrates, amino acids, nucleotides, trimethylamine-N-oxide, ethanolamine, betaine, and ethanol. Furthermore, thimerosal exposure affects various metabolic pathways, impairing the nervous system, disrupting protein metabolism, and potentially causing oxidative damage. Therefore, adopting a metabolomics approach in this investigation provided insights into the potentially implicated metabolic pathways contributing to the deleterious effects of thimerosal in biological systems.

Production of levan from Bacillus subtilis var. natto and apoptotic effect on SH-SY5Y neuroblastoma cells.

Levan is a high-valued polysaccharide of fructose produced by several microbial species. These polysaccharides have been described as effective therapeutic agents in some human disease conditions, such as cancer, heart diseases and diabetes. The objective of this study was to examine the effect of levan (ß-(2 â†’ 6)-fructan) produced through sucrose fermentation by B. subtilis var. natto on the proliferation rate, cytotoxicity, and apoptosis of human neuroblastoma SH-SY5Y cells. It was obtained 41.44 g/L of levan in 18 h by biotechnological fermentation and SH-SY5Y cells were exposed to 1000 µg/mL of levan. The treatment with 1000 µg/mL of levan induced apoptosis in SH-SY5Y cancer cells by the significant increase in Annexin V/7-AAD and caspase 3/7 activation, but did not decrease proliferation or triggered a cytotoxic effect. 1000 µg/mL levan treatment is a promising therapeutic strategy for SH-SY5Y neuroblastoma cells.