1.
Gene
; 35(1-2): 209-15, 1985.
Artigo
em Inglês
| MEDLINE
| ID: mdl-3161783
RESUMO
Pleurodeles waltlii genomic DNA has been cloned using several phage lambda vectors. We have isolated approx. 600 000 clones, which correspond to about 20% of the total DNA sequences of this organism. This constitutes the first large gene library of a Urodele. The low yield of cloning was attributable to the abundance of highly repetitive sequences, since recombinations in the bacterial host could lead to the loss of clones. Indeed, the existence of highly repetitive sequences was directly demonstrated by hybridization between recombinants and the total genome, and some of the cloned DNA was found to be unstable. We suggest new methods for cloning the highly repetitive sequences.
Assuntos
Clonagem Molecular , DNA/genética , Pleurodeles/genética , Sequências Repetitivas de Ácido Nucleico , Salamandridae/genética , Animais , Bacteriófago lambda/genética , Escherichia coli/genética , Amplificação de Genes , Vetores Genéticos
2.
C R Acad Hebd Seances Acad Sci D
; 269(20): 2048-50, 1969 Nov 17.
Artigo
em Francês
| MEDLINE
| ID: mdl-4983259
3.
Eur J Biochem
; 39(2): 525-39, 1973 Nov 15.
Artigo
em Inglês
| MEDLINE
| ID: mdl-4359630