RESUMO
PREMISE: Glandular trichomes are implicated in direct and indirect defense of plants. However, the degree to which glandular and non-glandular trichomes have evolved as a consequence of herbivory remains unclear, because their heritability, their association with herbivore resistance, their trade-offs with one another, and their association with other functions are rarely quantified. METHODS: We conducted a phylogenetic comparison of trichomes and herbivore resistance against the generalist caterpillar, Heliothis virescens, among tarweed species (Asteraceae: Madiinae) and a genetic correlation study comparing those same traits among maternal half-sibs of three tarweed species. RESULTS: Within a tarweed species, we found no evidence that herbivore growth rate decreased on tarweed individuals or maternal sib groups with more glandularity or denser trichomes. However, tarweed species with more glandularity and fewer non-glandular trichomes resulted in slower-growing herbivores. Likewise, a trade-off between glandular and non-glandular trichomes was apparent among tarweed species, but not among individuals or sib groups within a species. CONCLUSIONS: Our results suggest that this key herbivore does not select for trichomes as a direct defense in tarweed species. However, trichomes differed substantially among species and likely affect herbivore pressure on those species. Our results demonstrate that trade-offs among plant traits, as well as inference on the function of those traits, can depend on scale.
Assuntos
Asteraceae , Herbivoria , Filogenia , PlantasRESUMO
The production of herbivore-induced plant volatiles (HIPVs) is a type of indirect defense used by plants to attract natural enemies and reduce herbivory by insect pests. In many crops little is known about genotypic variation in HIPV production or how this may affect natural enemy attraction. In this study, we identified and quantified HIPVs produced by 10 sorghum (Sorghum bicolor) cultivars infested with a prominent aphid pest, the sorghum aphid (Melanaphis sorghi Theobald). Volatiles were collected using dynamic headspace sampling techniques and identified and quantified using GC-MS. The total amounts of volatiles induced by the aphids did not differ among the 10 cultivars, but overall blends of volatiles differed significantly in composition. Most notably, aphid herbivory induced higher levels of methyl salicylate (MeSA) emission in two cultivars, whereas in four cultivars, the volatile emissions did not change in response to aphid infestation. Dual-choice olfactometer assays were used to determine preference of the aphid parasitoid, Aphelinus nigritus, and predator, Chrysoperla rufilabris, between plants of the same cultivar that were un-infested or infested with aphids. Two aphid-infested cultivars were preferred by natural enemies, while four other cultivars were more attractive to natural enemies when they were free of aphids. The remaining four cultivars elicited no response from parasitoids. Our work suggests that genetic variation in HIPV emissions greatly affects parasitoid and predator attraction to aphid-infested sorghum and that screening crop cultivars for specific predator and parasitoid attractants has the potential to improve the efficacy of biological control.
Assuntos
Afídeos , Herbivoria , Sorghum , Compostos Orgânicos Voláteis , Afídeos/fisiologia , Animais , Sorghum/metabolismo , Sorghum/química , Sorghum/parasitologia , Compostos Orgânicos Voláteis/metabolismo , Compostos Orgânicos Voláteis/farmacologia , Compostos Orgânicos Voláteis/análise , Compostos Orgânicos Voláteis/química , Cromatografia Gasosa-Espectrometria de Massas , Salicilatos/metabolismo , Salicilatos/farmacologia , Vespas/fisiologiaRESUMO
Associational resistance (AR) is a positive interaction in which a plant suffers less herbivore damage due to its association with a protective plant. Here, we evaluated whether plants with extra-floral nectaries (EFNs) can share indirect defenses with neighboring plants. We sampled 45 individuals of an EFN-bearing liana (Smilax polyantha) and recorded whether their support species had EFNs. In S. polyantha, we measured foliar herbivory and flower and fruit production. We examined the ant species composition and visitation of S. polyantha and whether they changed according to the supporting plant type (with or without EFNs). We experimentally determined whether S. polyantha supplemented with artificial nectaries could share indirect defenses with defenseless neighboring plants. Support plants with EFNs indirectly benefited S. polyantha by sharing mutualistic ant species. Smilax polyantha supported by plants with EFNs had a more specific ant species composition, a higher number of visiting ants and ant species richness, and exhibited nearly 3 times less foliar herbivory. However, we did not observe differences in fruit production between the two groups of S. polyantha. Finally, we observed that S. polyantha with artificial nectaries increased ant visitation on neighboring plants 2.5 times. We provide evidence that interspecific neighbors with EFNs can experience reciprocal benefits by sharing indirect defenses. Such local effects might escalate and affect the structure of plant communities.
Assuntos
Formigas , Humanos , Animais , Árvores , Néctar de Plantas , Plantas , Flores , SimbioseRESUMO
Many herbivorous insects die of pathogen infections, though the role of plant traits in promoting the persistence of these pathogens as an indirect interaction is poorly understood. We tested whether winter leaf retention of bush lupines (Lupinus arboreus) promotes the persistence of a nucleopolyhedroviruses, thereby increasing the infection risk of caterpillars (Arctia virginalis) feeding on the foliage during spring. We also investigated whether winter leaf retention reduces viral exposure of younger caterpillars that live on the ground, as leaf retention prevents contaminated leaves from reaching the ground. We surveyed winter leaf retention of 248 lupine bush canopies across twelve sites and examined how it related to caterpillar infection risk, herbivory, and inflorescence density. We also manipulated the amount of lupine litter available to young caterpillars in a feeding experiment to emulate litterfall exposure in the field. Greater retention of contaminated leaves from the previous season increased infection rates of caterpillars in early spring. Higher infection rates reduced herbivory and increased plant inflorescence density by summer. Young caterpillars exposed to less litterfall were more likely to starve to death but less likely to die from infection, further suggesting foliage mediated exposure to viruses. We speculate that longer leaf life span may be an unrecognized trait that indirectly mediates top-down control of herbivores by facilitating epizootics.
Assuntos
Herbivoria , Viroses , Animais , Larva , Longevidade , Insetos , Plantas , Folhas de PlantaRESUMO
Tanacetum cinerariifolium was known to produce pyrethrins, but the mechanism of pyrethrin biosynthesis was largely unclear. The author showed that the nonmevalonate and oxylipin pathways underlie biosynthesis of the acid and alcohol moieties, respectively, and a GDSL lipase joins the products of these pathways. A blend of the green leaf volatiles and (E)-ß-farnesene mediates the induction of wounding responses to neighboring intact conspecies by enhancing pyrethrin biosynthesis. Plants fight against herbivores underground as well as aboveground, and, in soy pulps, some fungi produce compounds selectively modulating ion channels in insect nervous system. The author proposed that indirect defense of plants occurs where microorganisms produce defense substances in the rhizosphere. Broad-spectrum pesticides, including neonicotinoids, may affect nontarget organisms. The author discovered cofactors enabling functional expression of insect nicotinic acetylcholine receptors (nAChRs). This led to understanding the mechanism of insect nAChR-neonicotinoid interactions, thus paving new avenues for controlling crop pests and disease vectors.
Assuntos
PiretrinasRESUMO
Insect-associated microbes, including pathogens, parasites, and symbionts, influence the interactions of herbivorous insects and pollinators with their host plants. Moreover, herbivory-induced changes in plant resource allocation and defensive chemistry can influence pollinator behavior. This suggests that the outcomes of interactions between herbivores, their microbes and host plants could have implications for pollinators. As epizootic diseases occur at high population densities, pathogen and parasite-mediated effects on plants could have landscape-level impacts on foraging pollinators. The goal of this minireview is to highlight the potential for an herbivore's multitrophic interactions to trigger plant-mediated effects on the immunity and health of pollinators. We highlight the importance of plant quality and gut microbiomes in bee health, and how caterpillars as model herbivores interact with pathogens, parasites, and symbionts to affect plant quality, which forms the centerpiece of multitrophic interactions between herbivores and pollinators. We also discuss the impacts of other herbivore-associated factors, such as agricultural inputs aimed at decreasing herbivorous pests, on pollinator microbiomes.
Assuntos
Herbivoria , Interações Hospedeiro-Patógeno , Insetos , Microbiota/fisiologia , Fenômenos Fisiológicos Vegetais , Polinização , Animais , Interações Hospedeiro-Parasita , Insetos/microbiologia , Insetos/parasitologia , Insetos/fisiologiaRESUMO
Indirect defence, the adaptive top-down control of herbivores by plant traits that enhance predation, is a central component of plant-herbivore interactions. However, the scope of interactions that comprise indirect defence and associated ecological and evolutionary processes has not been clearly defined. We argue that the range of plant traits that mediate indirect defence is much greater than previously thought, and we further organise major concepts surrounding their ecological functioning. Despite the wide range of plant traits and interacting organisms involved, indirect defences show commonalities when grouped. These categories are based on whether indirect defences boost natural enemy abundance via food or shelter resources, or, alternatively, increase natural enemy foraging efficiency via information or alteration of habitat complexity. The benefits of indirect defences to natural enemies should be further explored to establish the conditions in which indirect defence generates a plant-natural enemy mutualism. By considering the broader scope of plant-herbivore-natural enemy interactions that comprise indirect defence, we can better understand plant-based food webs, as well as the evolutionary processes that have shaped them.
Assuntos
Insetos , Plantas , Animais , Ecossistema , Cadeia Alimentar , HerbivoriaRESUMO
Plants produce species-specific herbivore-induced plant volatiles (HIPVs) after damage. We tested the hypothesis that herbivore-specific HIPVs prime neighboring plants to induce defenses specific to the priming herbivore. Since Manduca sexta (specialist) and Heliothis virescens (generalist) herbivory induced unique HIPV profiles in Nicotiana benthamiana, we used these HIPVs to prime receiver plants for defense responses to simulated herbivory (mechanical wounding and herbivore regurgitant application). Jasmonic acid (JA) accumulations and emitted volatile profiles were monitored as representative defense responses since JA is the major plant hormone involved in wound and defense signaling and HIPVs have been implicated as signals in tritrophic interactions. Herbivore species-specific HIPVs primed neighboring plants, which produced 2 to 4 times more volatiles and JA after simulated herbivory when compared to similarly treated constitutive volatile-exposed plants. However, HIPV-exposed plants accumulated similar amounts of volatiles and JA independent of the combination of priming or challenging herbivore. Furthermore, volatile profiles emitted by primed plants depended only on the challenging herbivore species but not on the species-specific HIPV profile of damaged emitter plants. This suggests that feeding by either herbivore species primed neighboring plants for increased HIPV emissions specific to the subsequently attacking herbivore and is probably controlled by JA.
Assuntos
Herbivoria/fisiologia , Nicotiana/imunologia , Nicotiana/metabolismo , Compostos Orgânicos Voláteis/metabolismo , Animais , Ciclopentanos/metabolismo , Manduca/fisiologia , Mariposas/fisiologia , Oxilipinas/metabolismo , Folhas de Planta/metabolismo , Nicotiana/parasitologiaRESUMO
Plants protect themselves against pest attack utilizing both direct and indirect modes of defense. The direct mode of defense includes morphological, biochemical, and molecular barriers that affect feeding, growth, and survival of herbivores whereas the indirect mode of defense includes release of a blend of volatiles that attract natural enemies of the pests. Both of these strategies adopted by plants are reinforced if the plants are supplied with one of the most abundant metalloids, silicon (Si). Plants absorb Si as silicic acid (Si(OH)4) and accumulate it as phytoliths, which strengthens their physical defense. This deposition of Si in plant tissue is up-regulated upon pest attack. Further, Si deposited in the apoplast, suppresses pest effector molecules. Additionally, Si up-regulates the expression of defense-related genes and proteins and their activity and enhances the accumulation of secondary metabolites, boosting induced molecular and biochemical defenses. Moreover, Si plays a crucial role in phytohormone-mediated direct and indirect defense mechanisms. It is also involved in the reduction of harmful effects of oxidative stress resulting from herbivory by accelerating the scavenging process. Despite increasing evidence of its multiple roles in defense against pests, the practical implications of Si for crop protection have received less attention. Here, we highlight recent developments in Si-mediated improved plant resistance against pests and its significance for future use in crop improvement.
Assuntos
Herbivoria , Silício , Defesa das Plantas contra Herbivoria , Reguladores de Crescimento de Plantas , PlantasRESUMO
Plants use volatile organic compounds (VOCs) to cue natural enemies to their herbivore prey on plants. Simultaneously, herbivores utilize volatile cues to identify appropriate hosts. Despite extensive efforts to understand sources of variation in plant communication by VOCs, we lack an understanding of how ubiquitous belowground mutualists, such as arbuscular mycorrhizal fungi (AMF), influence plant VOC emissions. In a full factorial experiment, we subjected plants of two milkweed (Asclepias) species under three levels of AMF availability to damage by aphids (Aphis nerii). We then measured plant headspace volatiles and chemical defenses (cardenolides) and compared these to VOCs emitted and cardenolides produced by plants without herbivores. We found that AMF have plant species-specific effects on constitutive and aphid-induced VOC emissions. High AMF availability increased emissions of total VOCs, two green leaf volatiles (3-hexenyl acetate and hexyl acetate), and methyl salicylate in A. curassavica, but did not affect emissions in A. incarnata. In contrast, aphids consistently increased emissions of 6-methyl-5-hepten-2-one and benzeneacetaldehyde in both species, independent of AMF availability. Both high AMF availability and aphids alone suppressed emissions of individual terpenes. However, aphid damage on plants under high AMF availability increased, or did not affect, emissions of those terpenes. Lastly, aphid feeding suppressed cardenolide concentrations only in A. curassavica, and AMF did not affect cardenolides in either plant species. Our findings suggest that by altering milkweed VOC profiles, AMF may affect both herbivore performance and natural enemy attraction.
Assuntos
Afídeos/fisiologia , Asclepias/química , Micorrizas/fisiologia , Compostos Orgânicos Voláteis/análise , Animais , Asclepias/metabolismo , Asclepias/parasitologia , Cardenolídeos/análise , Cromatografia Gasosa-Espectrometria de Massas , Herbivoria , Interações entre Hospedeiro e Microrganismos , Interações Hospedeiro-Parasita , Folhas de Planta/química , Folhas de Planta/metabolismo , Folhas de Planta/parasitologia , Análise de Componente PrincipalRESUMO
Tritrophic interactions between plants, herbivores, and their natural enemies are an integral part of all terrestrial ecosystems. Herbivore-induced plant volatiles (HIPVs) play a key role in these interactions, as they can attract predators and parasitoids to herbivore-attacked plants. Thirty years after this discovery, the ecological importance of the phenomena is widely recognized. However, the primary function of HIPVs is still subject to much debate, as is the possibility of using these plant-produced cues in crop protection. In this review, we summarize the current knowledge on the role of HIPVs in tritrophic interactions from an ecological as well as a mechanistic perspective. This overview focuses on the main gaps in our knowledge of tritrophic interactions, and we argue that filling these gaps will greatly facilitate efforts to exploit HIPVs for pest control.
Assuntos
Cadeia Alimentar , Herbivoria , Insetos , Plantas/metabolismo , Compostos Orgânicos Voláteis , Agricultura , AnimaisRESUMO
The disadvantage of induced defenses compared with constitutive defenses is the time during which a plant is vulnerable to herbivory before activation. There is obvious importance in determining the costs and benefits of induced defenses. Some plants produce extrafloral nectaries (EFNs), which attract ants that protect against herbivores, and induce EFNs and extrafloral nectar in response to leaf damage. To understand induction of indirect defense by ants, we investigated the induction and relaxation of extrafloral nectar secretion and EFN formation after artificial leaf damage in young Mallotus japonicus. Plants were grown under control or leaf damage conditions a greenhouse or in the field. Following artificial leaf damage, we assessed secretion of extrafloral nectar and the number of ant workers on plants. We measured the number of EFNs on each of seven leaves produced after leaf damage. Extrafloral nectar secretion was induced within 1 day following leaf damage, resulting in the attraction of numerous ant workers, and the extrafloral nectar secretion decreased to initial levels after 7 days. The number of EFNs was largest on the first leaf and smallest on the sixth leaf produced after leaf damage, but the total number of EFNs did not differ between treatments. Thus, M. japonicus rapidly induces extrafloral nectar secretion after leaf damage, followed by relaxation. Furthermore, following induction of EFNs on newly produced leaves, it may decrease the cost of induction by reducing the number of EFNs on leaves produced later.
Assuntos
Formigas/fisiologia , Herbivoria , Mallotus (Planta)/fisiologia , Néctar de Plantas/metabolismo , Animais , Cadeia Alimentar , Folhas de Planta/fisiologiaRESUMO
Attraction of parasitoids to plant volatiles induced by multiple herbivory depends on the specific combinations of attacking herbivore species, especially when their feeding modes activate different defense signalling pathways as has been reported for phloem feeding aphids and tissue feeding caterpillars. We studied the effects of pre-infestation with non-host aphids (Brevicoryne brassicae) for two different time periods on the ability of two parasitoid species to discriminate between volatiles emitted by plants infested by host caterpillars alone and those emitted by plants infested with host caterpillars plus aphids. Using plants originating from three chemically distinct wild cabbage (Brassica oleracea) populations, Diadegma semiclausum switched preference for dually infested plants to preference for plants infested with Plutella xylostella hosts alone when the duration of pre-aphid infestation doubled from 7 to 14 days. Microplitis mediator, a parasitoid of Mamestra brassicae caterpillars, preferred dually-infested plants irrespective of aphid-infestation duration. Separation of the volatile blends emitted by plants infested with hosts plus aphids or with hosts only was poor, based on multivariate statistics. However, emission rates of individual compounds were often reduced in plants infested with aphids plus hosts compared to those emitted by plants infested with hosts alone. This effect depended on host caterpillar species and plant population and was little affected by aphid infestation duration. Thus, the interactive effect of aphids and hosts on plant volatile production and parasitoid attraction can be dynamic and parasitoid specific. The characteristics of the multi-component volatile blends that determine parasitoid attraction are too complex to be deduced from simple correlative statistical analyses.
Assuntos
Brassica/química , Animais , Afídeos/efeitos dos fármacos , Afídeos/crescimento & desenvolvimento , Afídeos/fisiologia , Comportamento Animal/efeitos dos fármacos , Brassica/metabolismo , Cromatografia Gasosa , Interações Hospedeiro-Parasita/efeitos dos fármacos , Larva/fisiologia , Compostos Orgânicos Voláteis/análise , Compostos Orgânicos Voláteis/farmacologiaRESUMO
Under conditions of competition for light, which lead to the inactivation of the photoreceptor phytochrome B (phyB), the growth of shade-intolerant plants is promoted and the accumulation of direct anti-herbivore defenses is down-regulated. Little is known about the effects of phyB on emissions of volatile organic compounds (VOCs), which play a major role as informational cues in indirect defense. We investigated the effects of phyB on direct and indirect defenses in tomato (Solanum lycopersicum) using two complementary approaches to inactivate phyB: illumination with a low red to far-red ratio, simulating competition, and mutation of the two PHYB genes present in the tomato genome. Inactivation of phyB resulted in low levels of constitutive defenses and down-regulation of direct defenses induced by methyl jasmonate (MeJA). Interestingly, phyB inactivation also had large effects on the blends of VOCs induced by MeJA. Moreover, in two-choice bioassays using MeJA-induced plants, the predatory mirid bug Macrolophus pygmaeus preferred VOCs from plants in which phyB was inactivated over VOCs from control plants. These results suggest that, in addition to repressing direct defense, phyB inactivation has consequences for VOC-mediated tritrophic interactions in canopies, presumably attracting predators to less defended plants, where they are likely to find more abundant prey.
Assuntos
Herbivoria , Fitocromo B/metabolismo , Comportamento Predatório , Solanum lycopersicum/metabolismo , Solanum lycopersicum/parasitologia , Compostos Orgânicos Voláteis/metabolismo , Animais , Ciclopentanos/farmacologia , Análise Discriminante , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Análise dos Mínimos Quadrados , Lepidópteros/fisiologia , Solanum lycopersicum/genética , Solanum lycopersicum/imunologia , Oxilipinas/farmacologia , Folhas de Planta/efeitos dos fármacos , Folhas de Planta/fisiologia , Tricomas/ultraestruturaRESUMO
Terrestrial molluscs and insect herbivores play a major role as plant consumers in a number of ecosystems, but their direct and indirect interactions have hardly been explored. The omnivorous nature of slugs makes them potential disrupters of predator-prey relationships, as a direct threat to small insects and through indirect, plant-mediated effects. Here, we examined the effects of the presence of two species of slugs, Arion rufus (native) and A. vulgaris (invasive) on the survivorship of young Pieris brassicae caterpillars when feeding on Brassica rapa plants, and on plant attractiveness to the main natural enemy of P. brassicae, the parasitoid Cotesia glomerata. In two separate predation experiments, caterpillar mortality was significantly higher on plants co-infested with A. rufus or A. vulgaris. Moreover, caterpillar mortality correlated positively with slug mass and leaf consumption by A. vulgaris. At the third trophic level, plants infested with slugs and plants co-infested with slugs and caterpillars were far less attractive to parasitoids than plants damaged by caterpillars only, independently of slug species. Chemical analyses confirmed that volatile emissions, which provide foraging cues for parasitoids, were strongly reduced in co-infested plants. Our study shows that the presence of slugs has the potential to affect insect populations, directly via consumptive effects, and indirectly via changes in plant volatiles that result in a reduced attraction of natural enemies. The fitness cost for P. brassicae imposed by increased mortality in presence of slugs may be counterbalanced by the benefit of escaping its parasitoids.
Assuntos
Brassica/parasitologia , Borboletas/fisiologia , Comportamento Alimentar , Gastrópodes/fisiologia , Interações Hospedeiro-Parasita , Himenópteros/fisiologia , Animais , Comportamento Predatório , OlfatoRESUMO
The beneficial effects of direct predation by zoophytophagous biological control agents (BCAs), such as the mirid bug Nesidiocoris tenuis, are well-known. However, the benefits of zoophytophagous BCAs' relation with host plants, via induction of plant defensive responses, have not been investigated until recently. To date, only the females of certain zoophytophagous BCAs have been demonstrated to induce defensive plant responses in tomato plants. The aim of this work was to determine whether nymphs, adult females, and adult males of N. tenuis are able to induce defense responses in tomato plants. Compared to undamaged tomato plants (i.e., not exposed to the mirid), plants on which young or mature nymphs, or adult males or females of N. tenuis fed and developed were less attractive to the whitefly Bemisia tabaci, but were more attractive to the parasitoid Encarsia formosa. Female-exposed plants were more repellent to B. tabaci and more attractive to E. formosa than were male-exposed plants. When comparing young- and mature-nymph-exposed plants, the same level of repellence was obtained for B. tabaci, but mature-nymph-exposed plants were more attractive to E. formosa. The repellent effect is attributed to the signaling pathway of abscisic acid, which is upregulated in N. tenuis-exposed plants, whereas the parasitoid attraction was attributed to the activation of the jasmonic acid signaling pathway. Our results demonstrate that all motile stages of N. tenuis can trigger defensive responses in tomato plants, although these responses may be slightly different depending on the stage considered.
Assuntos
Hemípteros/parasitologia , Heterópteros/fisiologia , Interações Hospedeiro-Parasita/imunologia , Doenças das Plantas/imunologia , Solanum lycopersicum/imunologia , Animais , Cromatografia Líquida , Feminino , Hemípteros/crescimento & desenvolvimento , Solanum lycopersicum/crescimento & desenvolvimento , Solanum lycopersicum/parasitologia , Masculino , Espectrometria de Massas , Doenças das Plantas/parasitologia , Reguladores de Crescimento de Plantas , Reação em Cadeia da Polimerase em Tempo RealRESUMO
Plants secrete extrafloral nectar (EFN) as an induced defense against herbivores. EFN contains not only carbohydrates and amino acids but also pathogenesis-related proteins and other protective enzymes, making EFN an exclusive reward. EFN secretion is commonly induced after wounding, likely owing to a jasmonic acid-induced cell wall invertase, and is limited by phloem sucrose availability: Both factors control EFN secretion according to the optimal defense hypothesis. Non-ant EFN consumers include parasitoids, wasps, spiders, mites, bugs, and predatory beetles. Little is known about the relevance of EFN to the nutrition of its consumers and, hence, to the structuring of arthropod communities. The mutualism can be established quickly among noncoevolved (e.g., invasive) species, indicating its easy assembly is due to ecological fitting. Therefore, increasing efforts are directed toward using EFN in biocontrol. However, documentation of the importance of EFN for the communities of plants and arthropods in natural, invasive, and agricultural ecosystems is still limited.
Assuntos
Cadeia Alimentar , Insetos/fisiologia , Magnoliopsida/metabolismo , Ácaros/fisiologia , Néctar de Plantas/metabolismo , Aranhas/fisiologia , Animais , Comportamento Alimentar , Controle Biológico de Vetores , SimbioseRESUMO
Plant volatiles (PVs) mediate interactions between plants and arthropods, microbes and other plants, and are involved in responses to abiotic stress. PV emissions are therefore influenced by many environmental factors, including herbivore damage, microbial invasion, and cues from neighboring plants, and also light regime, temperature, humidity and nutrient availability. Thus, an understanding of the physiological and ecological functions of PVs must be based on measurements reflecting PV emissions under natural conditions. However, PVs are usually sampled in the artificial environments of laboratories or climate chambers. Sampling of PVs in natural environments is difficult, being limited by the need to transport, maintain and provide power to instruments, or use expensive sorbent devices in replicate. Ideally, PVs should be measured in natural settings with high replication, spatio-temporal resolution and sensitivity, and modest costs. Polydimethylsiloxane (PDMS), a sorbent commonly used for PV sampling, is available as silicone tubing for as little as 0.60 m(-1) (versus 100-550 each for standard PDMS sorbent devices). Small pieces of silicone tubing (STs) of various lengths from millimeters to centimeters may be added to any experimental setting and used for headspace sampling, with little manipulation of the organism or headspace. STs have sufficiently fast absorption kinetics and large capacity to sample plant headspaces over a timescale of minutes to hours, and thus can produce biologically meaningful 'snapshots' of PV blends. When combined with thermal desorption coupled to GC-MS (a 40-year-old widely available technology), use of STs yields reproducible, sensitive, spatio-temporally resolved quantitative data from headspace samples taken in natural environments.
Assuntos
Dimetilpolisiloxanos/química , Nicotiana/química , Óleos Voláteis/química , Adsorção , Botânica/instrumentação , Botânica/métodos , Cromatografia Gasosa-Espectrometria de Massas , Nicotiana/metabolismoRESUMO
Plant defense against herbivores may compromise attraction of mutualists, yet information remains limited about the mechanisms underlying such signaling tradeoffs. Here, we investigated the effects of foliar herbivory by two herbivore species on defense compounds, floral signaling, pollinator and parasitoid attraction, and seed production. Herbivory generally reduced the quantity of many floral volatile organic compounds VOCs) in Brassica rapa. By contrast, floral color, flower diameter, and plant height remained unaffected. The decreased amounts of floral volatiles led to reduced attractiveness of flowers to pollinators, but increased the attractiveness of herbivore-infested plants to parasitoids. Plants infested with the native butterfly Pieris brassicae produced more flowers during early flowering, effectively compensating for the lower olfactory attractiveness. Herbivory by the invasive Spodoptera littoralis increased the amounts of glucobrassicanapin, and led to delayed flowering. These plants tended to attract fewer pollinators and to produce fewer seeds. Our study indicates a tradeoff between pollinator attraction and indirect defense (parasitoid attraction), which can be mitigated by reduced floral VOC emission and production of more early flowers. We suggest that this compensatory mechanism is specific to plant-herbivore associations with a coevolutionary history.
Assuntos
Flores/fisiologia , Herbivoria , Polinização , Compostos Orgânicos Voláteis/química , Animais , Abelhas , Brassica rapa/fisiologia , Borboletas , Cor , Flores/anatomia & histologia , Flores/química , Spodoptera , Simbiose , VespasRESUMO
Exposure to herbivore-induced plant volatiles (HIPVs) is known to enhance the defense responses in plants. This so-called priming effect has only been marginally studied in intercropping systems. We tested whether HIPVs from cowpea, which often serves as an intercrop alongside maize, can prime herbivore-induced volatile emissions in maize. Conventional volatile collection assays and real-time mass spectrometry revealed that maize plants that were exposed to HIPVs from cowpea infested with Spodoptera exigua caterpillars emitted more than control plants when they themselves were subsequently damaged by the same pest. The enhanced emission was only evident on the first day after infestation. Maize plants that were exposed to HIPVs from cowpea infested by S. frugiperda larvae showed no priming effect and released considerably less upon S. frugiperda infestation than upon S. exigua infestation. The latter may be explained by the fact that S. frugiperda is particularly well adapted to feed on maize and is known to suppress maize HIPV emissions. Our results imply that HIPVs from cowpea, depending on the inducing insect herbivore, may strongly prime maize plants. This deserves further investigation, also in other intercropping systems, as it can have important consequences for tritrophic interactions and crop protection.