Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 1.063
Filtrar
Mais filtros

Tipo de documento
Intervalo de ano de publicação
1.
Int J Legal Med ; 135(3): 801-815, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33084995

RESUMO

During a homicide investigation in which fire has been used to reduce the size of the cadaver and conceal the evidence of injuries, the identification of perimortem trauma presents a challenge, in particular in cases when the perpetrator has dismembered the body followed by burning the remains. It is therefore important to understand the effects which heat causes on fresh bone. The aim of this paper is to perform a pilot study on the survival ratio of toolmarks in different anatomical regions associated with dismemberment, and a descriptive analysis of the variables that may potentially influence the post-burning survival and detection. To achieve this, three donated embalmed cadavers were used to simulate a case in which an attempted dismemberment and burning had occurred. Fifty-five pre-burning injuries were manually induced: 30 using a machete to inflict chopping trauma, and 25 with a serrated bread knife to inflict sharp force trauma, on the thigh, knee, ankle and wrist. The cadavers were cremated in a furnace at Madrid's Cementerio Sur and the burnt remains were analysed at the Laboratorio de Antropología y Odontología Forense of the Universidad Complutense de Madrid. Not all pre-burning injuries inflicted were visible after the cremation process; only 13% were detected in this experiment. Toolmarks can be masked, modified, destroyed or overlooked from the outset of the procedure due to several factors which influence the post-burning survival and detection of toolmarks and contribute to conceal the evidence of trauma. Additional research should be done to study further variables which affect the post-burning visibility of sharp force trauma.


Assuntos
Osso e Ossos/lesões , Desmembramento de Cadáver , Cremação , Ferimentos Penetrantes/patologia , Idoso , Humanos , Masculino , Pessoa de Meia-Idade , Projetos Piloto , Espanha , Armas
2.
ScientificWorldJournal ; 2021: 6622363, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33986635

RESUMO

INTRODUCTION: Injuries are of public health concern and the leading cause of residual disability and death among teenagers, especially in low- and middle-income countries (LMICs). In Ghana, the burden of injury among adolescents is under-reported. Hence, the study sought to determine the prevalence of serious injuries (SI) and the potential factors influencing these injuries among school children in Ghana. METHODS: This study was conducted in Ghana among Junior High School (JHS) and senior high school students (SHS) using the 2012 Global School-Based Student Health Survey (GSHS) data. The GSHS employed two-stage cluster sampling method. Serious injuries (SI) and independent factors were measured via self-administered questionnaires. Pearson chi-square test between each explanatory variable and serious injuries was conducted and the level of statistical significance was set at 5%. The significant variables from the chi-square test were selected for multiple logistic regression analysis. Multiple logistic regression was performed to estimate the adjusted odds ratio (AOR) at 95% confidence interval (CI). RESULTS: The prevalence of SI in the past 12 months was 66% [CI=61.8-70.2] . The most common cause of SI was fall, 36%. The common types of injuries were cut/stab wounds and broken/dislocated bone. In the multiple logistic regression analysis, after controlling for other variables, educational level (AOR = 0.64, CI = 0.44-0.90, p < 0.015), suicidal ideation (AOR = 1.58, CI = 1.00-2.48, p < 0.002), suicidal attempt (AOR = 1.88, CI = 1.29-2.72, p < 0.001), having at least one close friend (AOR = 1.49, CI = 1.17-1.89, p < 0.002), school truancy (AOR = 1.66, CI = 1.31-2.09, p < 0.000), smoking marijuana (AOR = 2.64, CI = 1.22-5.69), and amphetamine use (AOR = 2.95, CI = 1.46-5.69) were independently associated with SI. CONCLUSION: The findings of the study established a high prevalence of SI among adolescents in Ghana, with cut/stab wound and broken/dislocated bone being the most reported type of injuries. This study also revealed that factors such as educational level, suicidal ideation, suicidal attempt, at least one close friend, school truancy, smoking marijuana, and amphetamine use are associated with SI among the adolescents. Therefore, pragmatic interventional programs should be targeted at these factors to curb the rate of SI among junior and senior school students.


Assuntos
Acidentes por Quedas/estatística & dados numéricos , Transtornos Relacionados ao Uso de Anfetaminas/epidemiologia , Fraturas Ósseas/epidemiologia , Abuso de Maconha/epidemiologia , Ideação Suicida , Tentativa de Suicídio/estatística & dados numéricos , Ferimentos Penetrantes/epidemiologia , Absenteísmo , Adolescente , Criança , Escolaridade , Feminino , Fraturas Ósseas/patologia , Amigos/psicologia , Gana/epidemiologia , Inquéritos Epidemiológicos , Humanos , Masculino , Razão de Chances , Prevalência , Instituições Acadêmicas , Estudantes/psicologia , Ferimentos Penetrantes/patologia , Adulto Jovem
3.
Int J Legal Med ; 134(3): 1133-1140, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32162009

RESUMO

Sharp force trauma is routinely encountered in forensic practice. Often the question is posed, how much pressure or energy would have been necessary to inflict a cut with a specific knife, in order to further characterize the perpetrator or determine his intent to cause harm. This paper investigates two knife blades and its individual pressures needed to cut through the epidermis and dermis. In order to examine the necessary force for cutting through the skin, we performed experiments on a piglet skin-on-gelatin phantom. Two similarly small knives-a paring knife with a serrated blade and a Swiss Army pocket knife with a smooth blade-were moved over the phantom surface using a mobile cutting apparatus with varying weight on the load arm with the knife. The depth of the cut was to be determined according to a scale from zero to three: grade 0 = no cut; grade 1 = cut into epidermis only; grade 2 = cut into dermis; grade 3 = complete transection of the entire skin. Each cut inflicted at a specific pressure force was assessed closely in order to ascertain depth, calculate the velocity, and ultimately determine the point at which a cut would inflict grade 3 damage. The smooth blade of a pocket knife needed at least 1900g pressure in order to slice through pig skin mounted on a thick gelatin block, whereas a serrated blade of a paring knife managed to cut into or through the dermis at a comparatively lower force of 700g. Our study shows that at the same cutting velocity, a significant difference in pressure is necessary to inflict the same degree of damage.


Assuntos
Pressão , Pele/lesões , Armas , Ferimentos Penetrantes/patologia , Animais , Gelatina , Humanos , Modelos Biológicos , Suínos
4.
Int J Legal Med ; 134(3): 1023-1032, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-31522297

RESUMO

Micro-CT has successfully been applied to the characterization of false starts (FSs) and, among several parameters, kerf width seems to correlate to the tool blade. The aim of the present study was to evaluate the diagnostic value of micro-CT for the differentiation of saw marks produced by different classes of saws and by saws belonging to the same class and differing only in number of teeth per inches (TPI). A morphological and morphometric analysis of 84 marks, produced by 6 saws belonging to 3 morphological classes, was performed. In the experimental cohort, for each parameter, statistically significant differences in intra- and inter-class analysis were searched for and cut-offs were established. The diagnostic accuracy of cut-offs was assessed through statistical analyses on the validation cohort. The morphological assessment did not allow to differentiate saws differing only in TPI. Angle 1 and top kerf width, respectively for cross-cut and rip-cut saws with alternating set, allowed a good discrimination between FSs produced by tools belonging to the same morphological class. High positive predictive values were found in intra-class analyses, while results in inter-class analyses were less encouraging. In cases of dismemberment, a micro-CT-based analysis of FSs is strongly suggested as a part of a multistep and multimodal methodology of assessment, which includes scanning electron microscopy and stereomicroscopy. A morphological assessment should be followed by an experimental trial and by a morphometric analysis. Further studies are needed to evaluate hampering factors, such as damage by physico-chemical agents.


Assuntos
Osso e Ossos/diagnóstico por imagem , Osso e Ossos/lesões , Osso e Ossos/patologia , Ferimentos Penetrantes/diagnóstico por imagem , Ferimentos Penetrantes/etiologia , Ferimentos Penetrantes/patologia , Microtomografia por Raio-X , Estudos de Coortes , Patologia Legal/métodos , Humanos , Curva ROC , Sensibilidade e Especificidade , Armas/classificação
5.
J Pathol ; 249(4): 435-446, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31342513

RESUMO

Diabetes induces dysregulation throughout the spectrum of myeloid lineage cells from progenitors to terminally differentiated cells. Another complication of diabetes is persistent inflammation, including prolonged accumulation of macrophages, which contributes to impaired wound healing. However, it remains unclear whether diabetes disrupts the response of bone marrow progenitors to peripheral injury and whether such dysregulation leads to sustained inflammation and impaired healing. Here, we demonstrated that diabetic mice (db/db, referred to here as DB) exhibit myeloid lineage bias during homeostasis and following injury. In addition, cells in the LSK (Lin- Sca-1+ cKit+ ) population of DB mice are preprogrammed towards myeloid commitment at the transcriptional level, and cultured myeloid progenitors from DB mice produce more monocytes ex vivo than their non-diabetic counterparts. We also show via bone marrow transfer between interleukin-1 receptor 1 KO (Il1r1-/- ) and DB mice that IL-1R1 signaling is likely not involved in myeloid skewing in DB mice. Furthermore, in vitro experiments indicated that macrophage colony-stimulating factor receptor signaling is not likely involved in enhanced myeloid transcription factor expression in LSK cells of DB mice. Our findings indicate that myeloid lineage commitment in bone marrow may contribute to increased macrophage numbers observed in diabetic skin wounds, and that strategies to regulate monopoiesis during homeostasis or post-wounding may improve diabetic wound healing. © 2019 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.


Assuntos
Linhagem da Célula , Diabetes Mellitus Tipo 2/patologia , Macrófagos/patologia , Células Progenitoras Mieloides/patologia , Pele/patologia , Cicatrização , Ferimentos Penetrantes/patologia , Animais , Transplante de Medula Óssea , Células Cultivadas , Diabetes Mellitus Tipo 2/genética , Diabetes Mellitus Tipo 2/metabolismo , Modelos Animais de Doenças , Macrófagos/metabolismo , Camundongos Endogâmicos C57BL , Camundongos Knockout , Células Progenitoras Mieloides/metabolismo , Mielopoese , Receptor de Fator Estimulador de Colônias de Macrófagos/metabolismo , Receptores Tipo I de Interleucina-1/genética , Receptores Tipo I de Interleucina-1/metabolismo , Transdução de Sinais , Pele/lesões , Pele/metabolismo , Transplante de Células-Tronco , Ferimentos Penetrantes/genética , Ferimentos Penetrantes/metabolismo
6.
Lasers Surg Med ; 52(6): 530-536, 2020 07.
Artigo em Inglês | MEDLINE | ID: mdl-31763712

RESUMO

BACKGROUND AND OBJECTIVE: Wound healing in diabetes mellitus (DM) patients is one of the major health concerns globally. Intense pulsed light (IPL) has been widely used in cosmetic dermatology via mechanisms involving fibroblast stimulation, collagen synthesis, and dermal remodeling, which are events that also occur during the process of wound healing. This present study was aimed to evaluate the possible beneficial effect of IPL on the wound healing in diabetic rats. MATERIALS AND METHODS: Diabetes was induced in Sprague-Dawley rats using streptozotocin. The rats were randomly divided into four groups: normal group, DM only group, DM rats with IPL treatment 2 weeks before wounding (DM + IPL-Pre group), and DM rats with concurrent IPL exposure and wounding (DM + IPL-Con group). The wounds were created on the dorsal skin of rats. Wound closure rate, collagen deposition, and angiogenesis were assessed. RESULTS: There were no significant differences in the wound closure rate and mean time to wound closure between IPL-treated diabetic rats and normal rats. By contrast, delayed wound closure and prolonged mean time to wound closure were both noticed in DM only group. Enhanced collagen deposition and angiogenesis were observed in IPL-Pre, but not IPL-Con diabetic rats, as compared with untreated DM rats. CONCLUSION: Results of this study may provide novel insight into future preventive strategies using IPL for the management of wounds in diabetic patients. Lasers Surg Med. © 2019 Wiley Periodicals, Inc.


Assuntos
Diabetes Mellitus Experimental/complicações , Terapia de Luz Pulsada Intensa , Úlcera Cutânea/terapia , Cicatrização/efeitos da radiação , Ferimentos Penetrantes/terapia , Animais , Masculino , Ratos , Ratos Sprague-Dawley , Úlcera Cutânea/etiologia , Úlcera Cutânea/patologia , Ferimentos Penetrantes/etiologia , Ferimentos Penetrantes/patologia
7.
Am J Forensic Med Pathol ; 41(4): 315-320, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-32804687

RESUMO

Crossbow fatalities are a rare occurrence, but crossbow use is on the rise. The manner of death in crossbow fatalities is overwhelmingly opined accident or suicide, not homicide. Despite their increasing use and reports of at least 14 crossbow-related homicides in the media for the last 5 years, crossbow homicides are rarely reported in the medical literature; only 10 articles that discussed 20 crossbow homicides were identified in the PubMed database. Here, we describe a case of a 20-year-old man who was found dead in his driveway after being shot in the abdomen with a crossbow by another person. The crossbow bolt had a mechanical 2-blade broadhead that transected the descending aorta and lodged in his vertebra. When completing a medicolegal death investigation and postmortem examination on suspected crossbow-related deaths, knowledge of crossbow components, its utility as a weapon, wound patterns, and how it can cause death are important. This case serves to build on the limited medical literature of crossbow homicides, educate forensic pathologists about the features of crossbow deaths, and highlight manner of death considerations.


Assuntos
Traumatismos Abdominais/patologia , Homicídio , Ferimentos Penetrantes/patologia , Traumatismos Abdominais/etiologia , Humanos , Masculino , Armas , Adulto Jovem
8.
Am J Forensic Med Pathol ; 41(4): 280-286, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-32852293

RESUMO

Scanning electron microscopy with energy-dispersive x-ray (SEM/EDX) analysis is an investigation whose potential has become increasingly important in the field of forensic research and diagnosis. We present the procedure to perform a well-carried-out SEM/EDX analysis on corpses affected by different types of injuries, such as blunt force trauma, ligature strangulation, electrocution, sharp force trauma, gunshot wounds, and intoxication. After the areas of forensic interest have been macroscopically identified, the sampling can be performed in 2 different ways: apposition of the double-sided graphite tape on the damaged area or performing the excision of a biological sample. In both cases, a proper negative control sample is required. In all cases, SEM/EDX analysis can detect exogenous microtraces consistent with the types of injuries involved. In blunt force trauma, microparticles of different nature deriving from the contact of the blunt instrument with the victim may be observed; in sharp force trauma, metal microtraces (Fe, Cr, Al, Ti) can be identified. In ligature strangulation, exogenous microtraces may be found in the cutaneous furrow. In electrocution, it allows to identify the pathognomonic metal pattern (Cu, Zn, Fe) of the "electric mark." In gunshot wounds, the main applications regards the detection of metal particles (Pb, Ba, Sb) of gunshot residues. Finally, in the analysis of intoxicants, it may identify traces of toxic substances. Thus, the authors conclude that SEM/EDX analysis can provide essential information to assist in the medicolegal investigation of death.


Assuntos
Medicina Legal/métodos , Microscopia Eletrônica de Varredura , Asfixia/patologia , Osso e Ossos/química , Osso e Ossos/patologia , Traumatismos por Eletricidade/patologia , Elementos Químicos , Humanos , Metais/análise , Lesões do Pescoço/patologia , Intoxicação/patologia , Pele/química , Pele/patologia , Ferimentos Penetrantes/patologia
9.
BMC Emerg Med ; 20(1): 17, 2020 03 09.
Artigo em Inglês | MEDLINE | ID: mdl-32151240

RESUMO

BACKGROUND: There are many high-volume trauma centers in limited resource environments where a thorough clinical examination of patients may contribute to a more economical, accurate, and widely applicable method of determining the proper management of patients with penetrating neck injuries. The purpose of this study was to validate thorough physical examination as a reliable diagnostic tool in these patients. METHODS: We performed an observational retrospective study of a diagnostic accuracy test where we compared clinical findings (symptoms and soft signs on admission of the patient) with the definitive findings according to the gold standard test for each particular situation (selective studies, clinical observation and surgical exploration). The study was conducted at Hospital Occidente Kennedy (HOK) between August 2009 and June 2010. RESULTS: The sample consisted of the clinical records of 207 (n = 207) patients who went to the emergency room for penetrating neck wounds at Hospital Occidente Kennedy (HOK). Of the total sample, 36.2% (n = 75) of patients were considered "asymptomatic" as they didn't present with any soft signs of injury. Vascular soft signs were present in 57% (n = 118) of the patients, soft signs of the airway and the upper gastrointestinal tract were present in 15.9% (n = 33) and 21.3% (n = 44) of the patients respectively. The sensitivity and negative predictive value (NPV) of any soft sign to determine injuries which require surgical repair was 97.4% [CI] [86.5-99.5%] and 98.7% [CI] [92.8-99.8%] respectively, with a range of confidence [CI] of 95%. CONCLUSIONS: Our study's main findings suggest that patients with neck injuries and no vascular, airway, or gastrointestinal soft sign can be safely managed with a conservative approach. It is important to emphasize the value of the clinical examination since there are many contexts in the modern world where a considerable amount of the population is afflicted by neck trauma and treated under conditions where technological resources are limited.


Assuntos
Lesões do Pescoço/diagnóstico , Exame Físico/normas , Ferimentos Penetrantes/diagnóstico , Adulto , Feminino , Humanos , Masculino , Lesões do Pescoço/patologia , Lesões do Pescoço/terapia , Estudos Retrospectivos , Índices de Gravidade do Trauma , Ferimentos Penetrantes/patologia , Ferimentos Penetrantes/terapia
10.
Int J Mol Sci ; 21(19)2020 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-32998232

RESUMO

Estrogenic steroids and adenosine A2A receptors promote the wound healing and angiogenesis processes. However, so far, it is unclear whether estrogen may regulate the expression and pro-angiogenic activity of A2A receptors. Using in vivo analyses, we showed that female wild type (WT) mice have a more rapid wound healing process than female or male A2A-deficient mice (A2AKO) mice. We also found that pulmonary endothelial cells (mPEC) isolated from female WT mice showed higher expression of A2A receptor than mPEC from male WT mice. mPEC from female WT mice were more sensitive to A2A-mediated pro-angiogenic response, suggesting an ER and A2A crosstalk, which was confirmed using cells isolated from A2AKO. In those female cells, 17ß-estradiol potentiated A2A-mediated cell proliferation, an effect that was inhibited by selective antagonists of estrogen receptors (ER), ERα, and ERß. Therefore, estrogen regulates the expression and/or pro-angiogenic activity of A2A adenosine receptors, likely involving activation of ERα and ERß receptors. Sexual dimorphism in wound healing observed in the A2AKO mice process reinforces the functional crosstalk between ER and A2A receptors.


Assuntos
Estradiol/farmacologia , Receptor alfa de Estrogênio/genética , Receptor beta de Estrogênio/genética , Neovascularização Fisiológica/efeitos dos fármacos , Receptor A2A de Adenosina/genética , Ferimentos Penetrantes/genética , Adenosina/análogos & derivados , Adenosina/farmacologia , Animais , Proliferação de Células/efeitos dos fármacos , Células Endoteliais/citologia , Células Endoteliais/efeitos dos fármacos , Células Endoteliais/metabolismo , Receptor alfa de Estrogênio/antagonistas & inibidores , Receptor alfa de Estrogênio/metabolismo , Receptor beta de Estrogênio/antagonistas & inibidores , Receptor beta de Estrogênio/metabolismo , Feminino , Regulação da Expressão Gênica , Pulmão/citologia , Pulmão/metabolismo , Masculino , Camundongos , Camundongos Knockout , Neovascularização Fisiológica/genética , Fenetilaminas/farmacologia , Pirazóis/farmacologia , Pirimidinas/farmacologia , Receptor Cross-Talk , Receptor A2A de Adenosina/metabolismo , Fatores Sexuais , Transdução de Sinais , Cicatrização/efeitos dos fármacos , Cicatrização/genética , Ferimentos Penetrantes/tratamento farmacológico , Ferimentos Penetrantes/metabolismo , Ferimentos Penetrantes/patologia
11.
Forensic Sci Med Pathol ; 16(2): 352-354, 2020 06.
Artigo em Inglês | MEDLINE | ID: mdl-31471867

RESUMO

In this paper we present a case of a 65-year-old man, with chronic kidney insufficiency treated with hemodialysis, found dead by his wife, lying in his bed. Based on autopsy findings, toxicological analysis, the police report and circumstances of death, it was concluded that the death was suicidal and that the cause of death was hemorrhagic shock due to fatal blood loss through a small puncture wound in an arteriovenous fistula of the left forearm, inflicted by a small Swiss Army knife. Similarly to occupation-related suicides, the victim had used his knowledge about chronic kidney disease and the relatively high pressure in an arteriovenous fistula to inform his suicidal actions, and, thus, this case might be considered a disease-knowledge-related or medical-knowledge-related suicide.


Assuntos
Fístula Arteriovenosa , Exsanguinação , Suicídio Consumado , Ferimentos Penetrantes/patologia , Idoso , Humanos , Masculino , Diálise Renal , Insuficiência Renal Crônica
12.
Forensic Sci Med Pathol ; 16(3): 540-543, 2020 09.
Artigo em Inglês | MEDLINE | ID: mdl-32125629

RESUMO

The carcass of a 15-year-old female Bottlenose dolphin (Tursiops aduncus) was retrieved from the Port River near Adelaide, South Australia, Australia. The animal was emaciated with five thick nylon fishing lines emerging from the oropharynx attached to a tangle of nylon and monofilament fishing line that also contained wire and eight fishing hooks. The mouth had been cut by the line and a circumferential curvilinear superficial abrasion/indentation from fishing line encircled the entire distal rostrum. Dissection of the upper aerodigestive tract revealed a large fish hook embedded in the lower blowhole associated with an adjacent abscess at the base of the epiglottis. The stomach contained two unattached fish hooks, parts of a plastic squid lure and two heavy duty work gloves. Further examination revealed a severe necrotising pneumonia with microabscesses in the kidneys and adrenal glands with scattered thromboemboli in keeping with terminal disseminated intravascular coagulation. Death had resulted from septic complications of fishing hook impalement and line entanglement with inanition. The case provides a graphic illustration of the effects of entanglement and fishing hook penetration, as well as ingestion of non-degradable plastic materials, in a free living Bottlenose dolphin.


Assuntos
Golfinho Nariz-de-Garrafa , Ferimentos Penetrantes/patologia , Animais , Austrália , Sepse/etiologia
13.
Forensic Sci Med Pathol ; 16(4): 721-723, 2020 12.
Artigo em Inglês | MEDLINE | ID: mdl-32519315

RESUMO

Fatal injuries caused by circular saws are rare and mainly self-inflicted. To the best of our knowledge this is the first recorded case of accidental death of a woman caused by an injury to the head region inflicted by a saw blade. The autopsy showed a 35 cm long wound, stretching from the right half of the jaw along the right side of the face and the right temporal region up to the parietal region. The cut went through the right hemisphere of the brain in the temporal and parietal lobe regions, damaging the sagittal sinus. Air embolism was considered to be the cause of death. Fatalities involving power tools, such as a circular saw, warrant a high degree of suspicion of criminal activity and require a comprehensive medicolegal investigation. Placing guards over the blade and around the driving system, i.e. the motor, belt, arbor, is of great importance for the prevention of potentially fatal injuries.


Assuntos
Acidentes Domésticos , Traumatismos Faciais/etiologia , Crânio/lesões , Ferimentos Penetrantes/etiologia , Idoso , Traumatismos Faciais/patologia , Feminino , Humanos , Crânio/patologia , Ferimentos Penetrantes/patologia
14.
J Cell Physiol ; 234(5): 7149-7160, 2019 05.
Artigo em Inglês | MEDLINE | ID: mdl-30370560

RESUMO

Fibroblast growth factor-2 (FGF-2) stimulates periodontal regeneration by a broad spectrum of effects on periodontal ligament (PDL) cells, such as proliferation, migration, and production of extracellular matrix. A critical factor in the success of periodontal regeneration is the rapid resolution of inflammatory responses in the tissue. We explored an anti-inflammatory effect of FGF-2 during periodontal regeneration and healing. We found that FGF-2 on mouse periodontal ligament cells (MPDL22) markedly downregulated CD40 expression, a key player of inflammation. In addition, FGF-2 inhibited CD40 signaling by the non-canonical nuclear factor-kappa B2 (NFκB2) pathway, resulting in decreased production of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α), which have the potential to recruit immune cells to inflamed sites. Furthermore, in vivo treatment of FGF-2 enhanced healing of skin wounds by counteracting the CD40-mediated inflammation. These results reveal that FGF-2 has an important function as a negative regulator of inflammation during periodontal regeneration and healing.


Assuntos
Anti-Inflamatórios/farmacologia , Antígenos CD40/metabolismo , Fator 2 de Crescimento de Fibroblastos/farmacologia , Ligamento Periodontal/efeitos dos fármacos , Periodontite/prevenção & controle , Animais , Antígenos CD40/genética , Linhagem Celular , Modelos Animais de Doenças , Interleucina-6/metabolismo , Masculino , Camundongos Endogâmicos BALB C , Subunidade p52 de NF-kappa B/metabolismo , Ligamento Periodontal/metabolismo , Ligamento Periodontal/patologia , Periodontite/genética , Periodontite/metabolismo , Periodontite/patologia , Transdução de Sinais , Fator de Necrose Tumoral alfa/metabolismo , Cicatrização/efeitos dos fármacos , Ferimentos Penetrantes/tratamento farmacológico , Ferimentos Penetrantes/metabolismo , Ferimentos Penetrantes/patologia
15.
Toxicol Appl Pharmacol ; 366: 17-24, 2019 03 01.
Artigo em Inglês | MEDLINE | ID: mdl-30684528

RESUMO

Ryanodine receptors have an important role in the regulation of intracellular calcium levels in the nervous system and muscle. It has been described that ryanodine receptors influence keratinocyte differentiation and barrier homeostasis. Our goal was to examine the role of ryanodine receptors in the healing of full-thickness dermal wounds by means of in vitro and in vivo methods. The effect of ryanodine receptors on wound healing, microcirculation and inflammation was assessed in an in vivo mouse wound healing model, using skin fold chambers in the dorsal region, and in HaCaT cell scratch wound assay in vitro. SKH-1 mice were subjected to sterile saline (n = 36) or ryanodine receptor agonist 4-chloro-m-cresol (0.5 mM) (n = 42) or ryanodine receptor antagonist dantrolene (100 µM) (n = 42). Application of ryanodine receptor agonist 4-chloro-m-cresol did not influence the studied parameters significantly, whereas ryanodine receptor antagonist dantrolene accelerated the wound closure. Inhibition of the calcium channel also increased the vessel diameters in the wound edges during the process of healing and increased the blood flow in the capillaries at all times of measurement. Furthermore, application of dantrolene decreased xanthine-oxidoreductase activity during the inflammatory phase of wound healing. Inhibition of ryanodine receptor-mediated effects positively influence wound healing. Thus, dantrolene may be of therapeutic potential in the treatment of wounds.


Assuntos
Bloqueadores dos Canais de Cálcio/farmacologia , Dantroleno/farmacologia , Queratinócitos/efeitos dos fármacos , Canal de Liberação de Cálcio do Receptor de Rianodina/efeitos dos fármacos , Pele/efeitos dos fármacos , Cicatrização/efeitos dos fármacos , Ferimentos Penetrantes/tratamento farmacológico , Animais , Velocidade do Fluxo Sanguíneo , Sinalização do Cálcio/efeitos dos fármacos , Linhagem Celular , Proliferação de Células/efeitos dos fármacos , Modelos Animais de Doenças , Humanos , Queratinócitos/metabolismo , Queratinócitos/patologia , Masculino , Camundongos Pelados , Microcirculação/efeitos dos fármacos , Espécies Reativas de Oxigênio/metabolismo , Canal de Liberação de Cálcio do Receptor de Rianodina/metabolismo , Pele/irrigação sanguínea , Pele/lesões , Pele/metabolismo , Fatores de Tempo , Ferimentos Penetrantes/metabolismo , Ferimentos Penetrantes/patologia , Ferimentos Penetrantes/fisiopatologia , Xantina Desidrogenase/metabolismo
16.
Dermatol Ther ; 32(1): e12779, 2019 01.
Artigo em Inglês | MEDLINE | ID: mdl-30371977

RESUMO

Although alginate has been known to be a good wound dressing, it does not have antimicrobial properties, has low availability, and is expensive. To overcome these problems, the present study was conducted, where the extraction of this material from an available small factory Pseudomonas aeruginosa and the improvement of its wound healing property by its combination with herb extract, Alhagi maurorum, done. Nineteen P. aeruginosa strains were isolated and identified from burned skin, and the one isolated strain with the highest ability of alginate production was selected. A. maurorum aqueous extract was prepared, and the toxicity of each material was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide assay. A mixture of nontoxic doses of each substance was then prepared. Thirty-two Wistar rats were divided into four groups (n = 8). The control group and the rest three groups, which were treated by alginate, A. maurorum extract, and alginate- A. maurorum extract. Throughout the 21 days of treatment, the open wound sites were checked. Finally, the rats were sacrificed and the effect of each substance on their skin tissue was evaluated. The results showed that the high alginate production without any toxic effect was obtained from the P. aeruginosa strain K1. A. maurorum aqueous extract had dose-dependent toxicity. The aqueous solution of alginate- A. maurorum extract complex group showed the best wound healing activity in both macroscopic and microscopic examinations. Recent research has introduced a new type of wound dressing with high wound healing properties. This could decrease the time for re-epithelialization and increase wound contraction percentage.


Assuntos
Alginatos/administração & dosagem , Fabaceae/química , Extratos Vegetais/farmacologia , Pseudomonas aeruginosa/química , Pele/efeitos dos fármacos , Cicatrização/efeitos dos fármacos , Ferimentos Penetrantes/tratamento farmacológico , Administração Cutânea , Alginatos/isolamento & purificação , Animais , Modelos Animais de Doenças , Combinação de Medicamentos , Géis , Masculino , Extratos Vegetais/isolamento & purificação , Ratos Wistar , Reepitelização/efeitos dos fármacos , Pele/lesões , Pele/patologia , Fatores de Tempo , Ferimentos Penetrantes/patologia
17.
Am J Forensic Med Pathol ; 40(2): 150-152, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30920406

RESUMO

Impalement injuries occur when a large foreign body traverses or penetrates a body cavity or extremity. Nowadays, impalement injuries are uncommon and are usually a consequence of a fall onto a blunt object or a road accident. The authors report a case of a woman found dead in her apartment, seemingly killed by a firearm injury to the abdomen. However, after the autopsy was carried out, it became clear that the injuries had the features of an impalement. After the inquiry, the murder weapon was identified as a battering ram, used by the robbers to break into the house of the woman. This is the first case ever described in literature where a battering ram has been used to impale someone. This case emphasizes the importance of an accurate description of injuries, also with photographs, in order to achieve effective recognition of the wound patterns as they may correspond to specific and unusual weapons.


Assuntos
Traumatismos Abdominais/patologia , Homicídio , Ferimentos Penetrantes/patologia , Idoso , Feminino , Humanos , Choque Hemorrágico/etiologia
18.
Am J Forensic Med Pathol ; 40(1): 61-64, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30475233

RESUMO

Postmortem computed tomography (PMCT) and PMCT angiography are increasingly used in forensic practice. However, their application in court is still limited because they need professional equipment or software to be displayed. In this article, the authors introduce 3-dimensional (3D) printing technology as a new approach to provide evidence in court. A 67-year-old woman suffered from severe sick sinus syndrome, and she was fitted with a ventricular demand inhibited pacemaker. The operation failed as the doctor accidentally perforated her heart with an electrode wire. Her heart stopped beating, and she died after the operation. Targeted PMCT with pulmonary artery angiography was performed after the body was dissected, and a model of the heart was reconstructed using 3D printing technology, with different colored materials. The results confirmed by the autopsy suggested that the victim had died of cardiac rupture. The apex close to the right margin of the heart showed a tiny cleft where the contrast agent was flowing out. The heart model was an effective way to interpret this medical injury. This 3D printing technology, combined with PMCT angiography, provides a convenient, efficient tool for identifying the cause of death. It could become a powerful form of court evidence in the future.


Assuntos
Angiografia por Tomografia Computadorizada , Traumatismos Cardíacos , Modelos Anatômicos , Impressão Tridimensional , Ferimentos Penetrantes , Idoso , Autopsia/métodos , Extravasamento de Materiais Terapêuticos e Diagnósticos , Feminino , Traumatismos Cardíacos/diagnóstico por imagem , Traumatismos Cardíacos/patologia , Humanos , Complicações Intraoperatórias , Erros Médicos , Marca-Passo Artificial/efeitos adversos , Ferimentos Penetrantes/diagnóstico por imagem , Ferimentos Penetrantes/patologia
19.
Adv Skin Wound Care ; 32(9): 1-5, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31403477

RESUMO

OBJECTIVE: To test the hypothesis that platelet-rich plasma (PRP) improves wound healing in rabbits by analyzing morphologic alterations in lesions with the use of PRP alone or combined with rosuvastatin (RSV). METHODS: Eight adult male New Zealand rabbits were used in this study. Researchers created four wounds on the back of each rabbit with an 8-mm punch. The control wound was treated with a 0.9% sodium chloride solution. Experimental wounds were treated with PRP, RSV, or both PRP and RSV. Dressings were changed with a new application of PRP and RSV every 4 days for 16 days. Wounds were biopsied on days 0, 7, 10, 14, and 17 for histopathologic evaluation of the scar tissue. MAIN RESULTS: Histopathology revealed reepithelialization in 100% of wounds treated with PRP alone after 17 days of treatment, compared with 50% of wounds treated with RSV alone and 75% of the wounds treated with PRP and RSV. Further, combining RSV and PRP reduced blood loss. The use of PRP alone induced 100% neovascularization, compared with 50% and 62.5% in wounds treated with PRP and RSV and RSV alone, respectively. All experimental wounds had a higher percentage of collagen fibers on day 17 postlesion when compared with control wounds (78.27% ± 4.69%). There were no significant differences among treatments; however, wounds treated with RSV alone had the lowest amount of collagen fibers (85.98% ± 3.51%). Wounds treated with PRP alone or PRP and RSV had 90.07% ± 6.20% and 90.76% ± 3.51% collagen fibers, respectively. CONCLUSIONS: The results of this study indicate that PRP elicits higher healing activity in the first 7 days postlesion. Treatments with RSV alone or RSV and PRP did enhance other healing phases.


Assuntos
Inibidores de Hidroximetilglutaril-CoA Redutases/uso terapêutico , Plasma Rico em Plaquetas , Rosuvastatina Cálcica/uso terapêutico , Úlcera Cutânea/terapia , Ferimentos Penetrantes/terapia , Animais , Modelos Animais de Doenças , Masculino , Coelhos , Úlcera Cutânea/etiologia , Úlcera Cutânea/patologia , Fatores de Tempo , Cicatrização , Ferimentos Penetrantes/etiologia , Ferimentos Penetrantes/patologia
20.
Ir Med J ; 112(2): 875, 2019 02 14.
Artigo em Inglês | MEDLINE | ID: mdl-30892853

RESUMO

Aim To highlight the dangers of hurling helmet modification. Methods A case report highlighting the consequences of modifying a hurling helmet from factory settings. Results Photographic evidence of a penetrating injury from helmet modification. Conclusion Rule changes to allow referees to inspect helmets before games take place.


Assuntos
Traumatismos em Atletas/etiologia , Traumatismos dos Dedos/etiologia , Dispositivos de Proteção da Cabeça/efeitos adversos , Lesões dos Tecidos Moles/etiologia , Ferimentos Penetrantes/etiologia , Traumatismos em Atletas/patologia , Traumatismos em Atletas/cirurgia , Traumatismos dos Dedos/patologia , Traumatismos dos Dedos/cirurgia , Humanos , Masculino , Lesões dos Tecidos Moles/patologia , Lesões dos Tecidos Moles/cirurgia , Resultado do Tratamento , Ferimentos Penetrantes/patologia , Ferimentos Penetrantes/cirurgia , Adulto Jovem
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA