Effect of marine heatwaves and warming on kelp microbiota influence trophic interactions.

The range-expansion of tropical herbivores due to ocean warming can profoundly alter temperate reef communities by overgrazing the seaweed forests that underpin them. Such ecological interactions may be mediated by changes to seaweed-associated microbiota in response to warming, but empirical evidence demonstrating this is rare. We experimentally simulated ocean warming and marine heatwaves (MHWs) to quantify effects on two dominant temperate seaweed species and their microbiota, as well as grazing by a tropical herbivore. The kelp Ecklonia radiata's microbiota in sustained warming and MHW treatments was enriched with microorganisms associated with seaweed disease and tissue degradation. In contrast, the fucoid Sargassum linearifolium's microbiota was unaffected by temperature. Consumption by the tropical sea-urchin Tripneustes gratilla was greater on Ecklonia where the microbiota had been altered by higher temperatures, while Sargassum's consumption was unaffected. Elemental traits (carbon, nitrogen), chemical defences (phenolics) and tissue bleaching of both seaweeds were generally unaffected by temperature. Effects of warming and MHWs on seaweed holobionts (host plus its microbiota) are likely species-specific. The effect of increased temperature on Ecklonia's microbiota and subsequent increased consumption suggest that changes to kelp microbiota may underpin kelp-herbivore interactions, providing novel insights into potential mechanisms driving change in species' interactions in warming oceans.

Multiscale stability of an intertidal kelp (Postelsia palmaeformis) near its northern range edge through a period of prolonged heatwaves.

BACKGROUND AND AIMS: Climate change, including gradual changes and extreme weather events, is driving widespread species losses and range shifts. These climatic changes are felt acutely in intertidal ecosystems, where many organisms live close to their thermal limits and experience the extremes of both marine and terrestrial environments. A recent series of multiyear heatwaves in the northeast Pacific Ocean might have impacted species even towards their cooler, northern range edges. Among them, the high intertidal kelp Postelsia palmaeformis has traits that could make it particularly vulnerable to climate change, but it is critically understudied. METHODS: In 2021 and 2022, we replicated in situ and aerial P. palmaeformis surveys that were conducted originally in 2006 and 2007, in order to assess the state of northern populations following recent heatwaves. Changes in P. palmaeformis distribution, extent, density and morphometrics were assessed between these two time points over three spatial scales, ranging from 250 m grid cells across the entire 167 km study region, to within grid cells and the individual patch. KEY RESULTS: We found evidence consistent with population stability at all three scales: P. palmaeformis remained present in all 250 m grid cells in the study region where it was previously found, and neither the extent within cells nor the patch density changed significantly between time points. However, there was evidence of slight distributional expansion, increased blade lengths and a shift to earlier reproductive timing. CONCLUSIONS: We suggest that apparent long-term stability of P. palmaeformis might be attributable to thermal buffering near its northern range edge and from the wave-exposed coastlines it inhabits, which may have decreased the impacts of heatwaves. Our results highlight the importance of multiscale assessments when examining changes within species and populations, in addition to the importance of dispersal capability and local conditions in regulating the responses of species to climate change.

Spatiotemporal variability in population demography and morphology of the habitat-forming macroalga Saccorhiza polyschides in the Western English Channel.

BACKGROUND AND AIMS: Large brown macroalgae serve as foundation organisms along temperate and polar coastlines, providing a range of ecosystem services. Saccorhiza polyschides is a warm-temperate kelp-like species found in the northeast Atlantic, which is suggested to have proliferated in recent decades across the southern UK, possibly in response to increasing temperatures, physical disturbance and reduced competition. However, little is known about S. polyschides with regard to ecological functioning and population dynamics across its geographical range. Here we examined the population demography of S. polyschides populations in southwest UK, located within the species' range centre, to address a regional knowledge gap and to provide a baseline against which to detect future changes. METHODS: Intertidal surveys were conducted during spring low tides at three sites along a gradient of wave exposure in Plymouth Sound (Western English Channel) over a period of 15 months. Density, cover, age, biomass and morphology of S. polyschides were quantified. Additionally, less frequent sampling of shallow subtidal reefs was conducted to compare intertidal and subtidal populations. KEY RESULTS: We recorded pronounced seasonality, with fairly consistent demographic patterns across sites and depths. By late summer, S. polyschides was a dominant habitat-former on both intertidal and subtidal reefs, with maximum standing stock exceeding 13 000 g wet weight m-2. CONCLUSIONS: Saccorhiza polyschides is a conspicuous and abundant member of rocky reef assemblages in the region, providing complex and abundant biogenic habitat for associated organisms and high rates of primary productivity. However, its short-lived pseudo-annual life strategy is in stark contrast to dominant long-lived perennial laminarian kelps. As such, any replacement or reconfiguration of habitat-forming macroalgae due to ocean warming will probably have implications for local biodiversity and community composition. More broadly, our study demonstrates the importance of high-resolution cross-habitat surveys to generate robust baselines of kelp population demography, against which the ecological impacts of climate change and other stressors can be reliably detected.

Syncopation and synchrony: Phenological dynamics of Pyropia nereocystis (Bangiophyceae) in central California.

Pyropia nereocystis is an annual northeastern Pacific-bladed bangialean species whose macroscopic stage epiphytized the annual canopy forming bull kelp Nereocystis luetkeana. I examined three in situ facets of these epiphyte-host dynamics in the central California region: (1) spatial and temporal variation in the presence of P. nereocystis epiphytes as a function of host density, (2) the relationship between individual host morphology and epiphytic P. nereocystis biomass, and (3) the ecophysiological growth ramifications for subtidal transplants of both life stages of P. nereocystis. Swath canopy surveys and whole host collections were conducted at five sites between November 2017 and February 2019. Additionally, transplants of P. nereocystis gametophytes and sporophytes were conducted across multiple subtidal depths. I observed temporal changes in the proportions of hosts epiphytized by P. nereocystis, with differences in seasonal persistence of P. nereocystis among sites and between years. Biomass of P. nereocystis was positively correlated with individual host stipe length, stipe surface area, and the primary principal component (PC) of stipe morphometrics denoted by principal component analysis (PCA). Gametogenesis in P. nereocystis epiphytes was spatially heterogeneous and limited for the 2018-2019 cohort due to comprehensive removal of hosts by the February 2019 sampling period. Transplants of P. nereocystis gametophytes yielded similar growth responses among depths, and sporophyte (conchocelis) transplant areal growth was positively correlated with transplant depth. These findings detail spatiotemporal complexity and multi-scale (individual, site, and whole region) phenological nuances for central Californian P. nereocystis epiphytes.

The effect of sedimentation on spore settlement and recruitment of the endemic Arctic kelp, Laminaria solidungula (Phaeophyceae).

Environmental changes associated with rapid climate change in the Arctic, such as the increased rates of sedimentation from climatic or anthropogenic sources, can enhance the impact of abiotic stressors on coastal ecosystems. High sedimentation rates can be detrimental to nearshore kelp abundance and distribution, possibly due to increased mortality at the spore settlement stage. Spore settlement and viability of the Arctic kelp Laminaria solidungula were examined through a series of lab-based sedimentation experiments. Spores were exposed to increasing sediment loads in three experimental designs simulating different sedimentation scenarios: sediment deposition above settled spores, settlement of spores on sediment-covered substrate, and simultaneous suspension of spores and sediments during settlement. Spore settlement was recorded upon completion of each experiment, and gametophyte abundance was assessed following a growth period with sediments removed to examine short-term spore viability via a gametophyte-to-settled-spore ratio. In all three types of sediment exposure, the addition of sediments caused a 30%-40% reduction in spore settlement relative to a no-sediment control. Spore settlement decreased significantly between the low and high sediment treatments when spores were settled onto sediment-covered substrates. In all experiments, increasing amounts of sediment had no significant effect on spore viability, indicating that spores that had settled under different short-term sediment conditions were viable. Our results indicate that depending on spore-sediment interaction type, higher rates of sedimentation resulting from increased sediment loading could affect L. solidungula spore settlement success with potential impacts on the long-term persistence of a diverse and productive benthic habitat.

Variation in body size drives spatial and temporal variation in lobster-urchin interaction strength.

How strongly predators and prey interact is both notoriously context dependent and difficult to measure. Yet across taxa, interaction strength is strongly related to predator size, prey size and prey density, suggesting that general cross-taxonomic relationships could be used to predict how strongly individual species interact. Here, we ask how accurately do general size-scaling relationships predict variation in interaction strength between specific species that vary in size and density across space and time? To address this question, we quantified the size and density dependence of the functional response of the California spiny lobster Panulirus interruptus, foraging on a key ecosystem engineer, the purple sea urchin Strongylocentrotus purpuratus, in experimental mesocosms. Based on these results, we then estimated variation in lobster-urchin interaction strength across five sites and 9 years of observational data. Finally, we compared our experimental estimates to predictions based on general size-scaling relationships from the literature. Our results reveal that predator and prey body size has the greatest effect on interaction strength when prey abundance is high. Due to consistently high urchin densities in the field, our simulations suggest that body size-relative to density-accounted for up to 87% of the spatio-temporal variation in interaction strength. However, general size-scaling relationships failed to predict the magnitude of interactions between lobster and urchin; even the best prediction from the literature was, on average, an order of magnitude (+18.7×) different than our experimental predictions. Harvest and climate change are driving reductions in the average body size of many marine species. Anticipating how reductions in body size will alter species interactions is critical to managing marine systems in an ecosystem context. Our results highlight the extent to which differences in size-frequency distributions can drive dramatic variation in the strength of interactions across narrow spatial and temporal scales. Furthermore, our work suggests that species-specific estimates for the scaling of interaction strength with body size, rather than general size-scaling relationships, are necessary to quantitatively predict how reductions in body size will alter interaction strengths.

Microclimate predicts kelp forest extinction in the face of direct and indirect marine heatwave effects.

Marine heatwaves threaten the persistence of kelp forests globally. However, the observed responses of kelp forests to these events have been highly variable on local scales. Here, we synthesize distribution data from an environmentally diverse region to examine spatial patterns of canopy kelp persistence through an unprecedented marine heatwave. We show that, although often overlooked, temperature variation occurring at fine spatial scales (i.e., a few kilometers or less) can be a critical driver of kelp forest persistence during these events. Specifically, though kelp forests nearly all persisted toward the cool outer coast, inshore areas were >3°C warmer at the surface and experienced extensive kelp loss. Although temperatures remained cool at depths below the thermocline, kelp persistence in these thermal refugia was strongly constrained by biotic interactions, specifically urchin populations that increased during the heatwave and drove transitions to urchin barrens in deeper rocky habitat. Urchins were, however, largely absent from mixed sand and cobble benthos, leading to an unexpected association between bottom substrate and kelp forest persistence at inshore sites with warm surface waters. Our findings demonstrate both that warm microclimates increase the risk of habitat loss during marine heatwaves and that biotic interactions modified by these events will modulate the capacity of cool microclimates to serve as thermal refugia.

Persistence of seaweed forests in the anthropocene will depend on warming and marine heatwave profiles.

Marine heatwaves (MHWs), discrete periods of extreme warm water temperatures superimposed onto persistent ocean warming, have increased in frequency and significantly disrupted marine ecosystems. While field observations on the ecological consequences of MHWs are growing, a mechanistic understanding of their direct effects is rare. We conducted an outdoor tank experiment testing how different thermal stressor profiles impacted the ecophysiological performance of three dominant forest-forming seaweeds. Four thermal scenarios were tested: contemporary summer temperature (22°C), low persistent warming (24°C), a discrete MHW (22-27°C), and temperature variability followed by a MHW (22-24°C, 22-27°C). The physiological performance of seaweeds was strongly related to thermal profile and varied among species, with the highest temperature not always having the strongest effect. MHWs were highly detrimental for the fucoid Phyllospora comosa, whereas the laminarian kelp Ecklonia radiata showed sensitivity to extended thermal stress and demonstrated a cumulative temperature threshold. The fucoid Sargassum linearifolium showed resilience, albeit with signs of decline with bleached and degraded fronds, under all conditions, with stronger decline under stable control and warming conditions. The varying responses of these three co-occurring forest-forming seaweeds under different temperature scenarios suggests that the impact of ocean warming on near shore ecosystems may be complex and will depend on the specific thermal profile of rising water temperatures relative to the vulnerability of different species.

On the ecology of Cystophora spp. forests.

Cystophora is the second largest genus of fucoids worldwide and, like many other forest-forming macroalgae, is increasingly threatened by a range of anthropogenic impacts including ocean warming. Yet, limited ecological information is available from the warm portion of their range (SW Western Australia), where severe range contractions are predicted to occur. Here, we provide the first insights on the abundance, diversity, productivity, and stand structure of Cystophora forests in this region. Forests were ubiquitous over more than 800 km of coastline and dominated sheltered and moderately-exposed reefs. Stand biomass and productivity were similar or greater than that of kelp forests in the temperate reef communities examined, suggesting that Cystophora spp. play a similarly important ecological role. The stand structure of Cystophora forests was, however, different than those of kelp forests, with most stands featuring an abundant bank of sub-canopy juveniles and only a few plants forming the canopy layer. Stand productivity followed an opposite seasonal pattern than that of kelps, with maximal growth in late autumn through early winter and net biomass loss in summer. Annually, stands contributed between 2.2 and 5.7 kg · m-2 (fresh biomass) to reef productivity depending on the dominant stand species. We propose that Cystophora forests play an important and unique role in supporting subtidal temperate diversity and productivity throughout temperate Australia, and urge a better understanding of their ecology and responses to anthropogenic threats.

SNP analyses reveal a diverse pool of potential colonists to earthquake-uplifted coastlines.

In species that form dense populations, major disturbance events are expected to increase the chance of establishment for immigrant lineages. Real-time tests of the impact of disturbance on patterns of genetic structure are, however, scarce. Central to testing these concepts is determining the pool of potential immigrants dispersing into a disturbed area. In 2016, a 7.8 magnitude earthquake occurred on the South Island of New Zealand. Affecting approximately 100 km of coastline, this quake caused extensive uplift (several metres high), extirpating many intertidal populations, including keystone intertidal kelp species. Following the uplift, we set out to determine the geographic origins of detached kelp specimens which rafted into the disturbed zone. Specifically, we used genotyping-by-sequencing (GBS) approaches to compare beach-cast southern bull-kelp (Durvillaea antarctica and Durvillaea poha) samples to established populations throughout the species' ranges, and thus infer the geographic origins of potential colonists reaching the disturbed coast. Our findings revealed an ongoing supply of diverse lineages dispersing to the newly uplifted coastline, suggesting potential for establishment of "exotic" lineages following disturbance. Furthermore, we found that some drifting individuals of each species came from far-distant regions, some >1,200 km away. These results show that diverse lineages - in many cases from very distant sources - can compete for new space in the wake of an exceptional disturbance event, illustrating the potential of long-distance dispersal as a key mechanism for reassembly of coastal ecosystems. Furthermore, our findings demonstrate that high-resolution genomic baselines can be used to robustly assign the provenance of dispersing individuals.

Responses of the kelp Saccharina latissima (Phaeophyceae) to the warming Arctic: from physiology to transcriptomics.

The Arctic region is currently facing substantial environmental changes due to global warming. Melting glaciers cause reduced salinity environments in coastal Arctic habitats, which may be stressful for kelp beds. To investigate the responses of the kelp Saccharina latissima to the warming Arctic, we studied the transcriptomic changes of S. latissima from Kongsfjorden (Svalbard, Norway) over a 24-hour exposure to two salinities (Absolute Salinity [SA ] 20 and 30) after a 7-day pre-acclimation at three temperatures (0, 8 and 15°C). In addition, corresponding physiological data were assessed during an 11-days salinity/temperature experiment. Growth and maximal quantum yield for photosystem II fluorescence were positively affected by increased temperature during acclimation, whereas hyposalinity caused negative effects at the last day of treatment. In contrast, hyposalinity induced marked changes on the transcriptomic level. Compared to the control (8°C - SA 30), the 8°C - SA 20 exhibited the highest number of differentially expressed genes (DEGs), followed by the 0°C - SA 20. Comparisons indicate that S. latissima tends to convert its energy from primary metabolism (e.g. photosynthesis) to antioxidant activity under hyposaline stress. The increase in physiological performance at 15°C shows that S. latissima in the Arctic region can adjust and might even benefit from increased temperatures. However, in Arctic fjord environments its performance might become impaired by decreased salinity as a result of ice melting.

Invasion-mediated effects on marine trophic interactions in a changing climate: positive feedbacks favour kelp persistence.

The interactive effects of ocean warming and invasive species are complex and remain a source of uncertainty for projecting future ecological change. Climate-mediated change to trophic interactions can have pervasive ecological consequences, but the role of invasion in mediating trophic effects is largely unstudied. Using manipulative experiments in replicated outdoor mesocosms, we reveal how near-future ocean warming and macrophyte invasion scenarios interactively impact gastropod grazing intensity and preference for consumption of foundation macroalgae ( Ecklonia radiata and Sargassum vestitum). Elevated water temperature increased the consumption of both macroalgae through greater grazing intensity. Given the documented decline of kelp ( E. radiata) growth at higher water temperatures, enhanced grazing could contribute to the shift from kelp-dominated to Sargassum-dominated reefs that is occurring at the low-latitude margins of kelp distribution. However, the presence of a native invader ( Caulerpa filiformis) was related to low consumption by the herbivores on dominant kelp at warmer temperatures. Thus, antagonistic effects between climate change and a range expanding species can favour kelp persistence in a warmer future. Introduction of species should, therefore, not automatically be considered unfavourable under climate change scenarios. Climatic changes are increasing the need for effective management actions to address the interactive effects of multiple stressors and their ecological consequences, rather than single threats in isolation.

Future climate change is predicted to affect the microbiome and condition of habitat-forming kelp.

Climate change is driving global declines of marine habitat-forming species through physiological effects and through changes to ecological interactions, with projected trajectories for ocean warming and acidification likely to exacerbate such impacts in coming decades. Interactions between habitat-formers and their microbiomes are fundamental for host functioning and resilience, but how such relationships will change in future conditions is largely unknown. We investigated independent and interactive effects of warming and acidification on a large brown seaweed, the kelp Ecklonia radiata, and its associated microbiome in experimental mesocosms. Microbial communities were affected by warming and, during the first week, by acidification. During the second week, kelp developed disease-like symptoms previously observed in the field. The tissue of some kelp blistered, bleached and eventually degraded, particularly under the acidification treatments, affecting photosynthetic efficiency. Microbial communities differed between blistered and healthy kelp for all treatments, except for those under future conditions of warming and acidification, which after two weeks resembled assemblages associated with healthy hosts. This indicates that changes in the microbiome were not easily predictable as the severity of future climate scenarios increased. Future ocean conditions can change kelp microbiomes and may lead to host disease, with potentially cascading impacts on associated ecosystems.

Seasonal and spatial variation in photosynthetic response of the kelp Ecklonia radiata across a turbidity gradient.

Understanding the photoacclimation response of macroalgae across broad spatial and temporal scales is necessary for predicting their vulnerability to environmental changes and quantifying their contribution to coastal primary production. This study investigated how the photosynthesis-irradiance response and photosynthetic pigment content of the kelp Ecklonia radiata varies both spatially and seasonally among seven sites located across a turbidity gradient in the Hauraki Gulf, north-eastern New Zealand. Photosynthesis-irradiance curves were derived under laboratory conditions for whole adult E. radiata using photorespirometry chambers. Lab-derived photosynthesis-irradiance curves in summer were also compared with in situ measurements made on kelp at each of the seven study sites. Photosynthetic parameters and pigments showed clear seasonal patterns across all sites as demonstrated by higher photosynthetic pigment levels and photosynthetic efficiency occurring in autumn and winter, and higher maximum rates of photosynthesis and respiration occurring in summer. Lamina biomass was similar across sites, yet thalli exhibited a clear photokinetic response to increasing turbidity. At turbid sites photosynthetic pigment levels and photosynthetic efficiency was higher, and respiration and saturation and compensation irradiances lower, compared to high-light sites. The results presented here further our understanding of low-light acclimation strategies in kelp and highlight the degree of seasonality in photosynthetic parameters. Though E. radiata demonstrates a clear capacity to photoacclimate to a degrading light environment, further research is needed to investigate the extent to which the observed acclimation can offset the likely negative effects of increasing turbidity on kelp forest primary production.

Mechanical properties of the wave-swept kelp Egregia menziesii change with season, growth rate and herbivore wounds.

The resistance of macroalgae to damage by hydrodynamic forces depends on the mechanical properties of their tissues. Although factors such as water-flow environment, algal growth rate and damage by herbivores have been shown to influence various material properties of macroalgal tissues, the interplay of these factors as they change seasonally and affect algal mechanical performance has not been worked out. We used the perennial kelp Egregia menziesii to study how the material properties of the rachis supporting a frond changed seasonally over a 2 year period, and how those changes correlated with seasonal patterns of the environment, growth rate and herbivore load. Rachis tissue became stiffer, stronger and less extensible with age (distance from the meristem). Thus, slowly growing rachises were stiffer, stronger and tougher than rapidly growing ones. Growth rates were highest in spring and summer when upwelling and long periods of daylight occurred. Therefore, rachis tissue was most resistant to damage in the winter, when waves were large as a result of seasonal storms. Herbivory was greatest during summer, when rachis growth rates were high. Unlike other macroalgae, E. menziesii did not respond to herbivore damage by increasing rachis tissue strength, but rather by growing in width so that the cross-sectional area of the wounded rachis was increased. The relative timing of environmental factors that affect growth rates (e.g. upwelling supply of nutrients, daylight duration) and of those that can damage macroalgae (e.g. winter storms, summer herbivore outbreaks) can influence the material properties and thus the mechanical performance of macroalgae.

Projected climate changes threaten ancient refugia of kelp forests in the North Atlantic.

Intraspecific genetic variability is critical for species adaptation and evolution and yet it is generally overlooked in projections of the biological consequences of climate change. We ask whether ongoing climate changes can cause the loss of important gene pools from North Atlantic relict kelp forests that persisted over glacial-interglacial cycles. We use ecological niche modelling to predict genetic diversity hotspots for eight species of large brown algae with different thermal tolerances (Arctic to warm temperate), estimated as regions of persistence throughout the Last Glacial Maximum (20,000 YBP), the warmer Mid-Holocene (6,000 YBP), and the present. Changes in the genetic diversity within ancient refugia were projected for the future (year 2100) under two contrasting climate change scenarios (RCP2.6 and RCP8.5). Models predicted distributions that matched empirical distributions in cross-validation, and identified distinct refugia at the low latitude ranges, which largely coincide among species with similar ecological niches. Transferred models into the future projected polewards expansions and substantial range losses in lower latitudes, where richer gene pools are expected (in Nova Scotia and Iberia for cold affinity species and Gibraltar, Alboran, and Morocco for warm-temperate species). These effects were projected for both scenarios but were intensified under the extreme RCP8.5 scenario, with the complete borealization (circum-Arctic colonization) of kelp forests, the redistribution of the biogeographical transitional zones of the North Atlantic, and the erosion of global gene pools across all species. As the geographic distribution of genetic variability is unknown for most marine species, our results represent a baseline for identification of locations potentially rich in unique phylogeographic lineages that are also climatic relics in threat of disappearing.

Under pressure: biomechanical limitations of developing pneumatocysts in the bull kelp (Nereocystis luetkeana, Phaeophyceae).

Maintaining buoyancy with gas-filled floats (pneumatocysts) is essential for some subtidal kelps to achieve an upright stature and compete for light . However, as these kelps grow up through the water column, pneumatocysts are exposed to substantial changes in hydrostatic pressure, which could cause complications as internal gases may expand or contract, potentially causing them to rupture, flood, and lose buoyancy. In this study, we investigate how pneumatocysts of Nereocystis luetkeana resist biomechanical stress and maintain buoyancy as they develop across a hydrostatic gradient. We measured internal pressure, material properties, and pneumatocyst geometry across a range of thallus sizes and collection depths to identify strategies used to resist pressure-induced mechanical failure. Contrary to expectations, all pneumatocysts had internal pressures less than atmospheric pressure, ensuring that thalli are always exposed to a positive pressure gradient and compressional loads, indicating that they are more likely to buckle than rupture at all depths. Small pneumatocysts collected from depths between 1 and 9 m (inner radius = 0.4-1.0 cm) were demonstrated to have elevated wall stresses under high compressive loads and are at greatest risk of buckling. Although small kelps do not adjust pneumatocyst material properties or geometry to reduce wall stress as they grow, they are ~3.4 times stronger than they need to be to resist hydrostatic buckling. When tested, pneumatocysts buckled around 35 m depth, which agrees with previous measures of lower limits due to light attenuation, suggesting that hydrostatic pressure may also define the lower limit of Nereocystis in the field.

Interactive effects of predator and prey harvest on ecological resilience of rocky reefs.

A major goal of ecosystem-based fisheries management is to prevent fishery-induced shifts in community states. This requires an understanding of ecological resilience: the ability of an ecosystem to return to the same state following a perturbation, which can strongly depend on species interactions across trophic levels. We use a structured model of a temperate rocky reef to explore how multi-trophic level fisheries impact ecological resilience. Increasing fishing mortality of prey (urchins) has a minor effect on equilibrium biomass of kelp, urchins, and spiny lobster predators, but increases resilience by reducing the range of predator harvest rates at which alternative stable states are possible. Size-structured predation on urchins acts as the feedback maintaining each state. Our results demonstrate that the resilience of ecosystems strongly depends on the interactive effects of predator and prey harvest in multi-trophic level fisheries, which are common in marine ecosystems but are unaccounted for by traditional management.

The variable routes of rafting: stranding dynamics of floating bull kelp Durvillaea antarctica (Fucales, Phaeophyceae) on beaches in the SE Pacific.

Dispersal on floating seaweeds depends on availability, viability, and trajectories of the rafts. In the southern hemisphere, the bull kelp Durvillaea antarctica is one of the most common floating seaweeds, but phylogeographic studies had shown low connectivity between populations from continental Chile, which could be due to limitations in local supply and dispersal of floating kelps. To test this hypothesis, the spatiotemporal dynamics of kelp strandings were examined in four biogeographic districts along the Chilean coast (28°-42°S). We determined the biomass and demography of stranded individuals on 33 beaches for three subsequent years (2013, 2014, 2015) to examine whether rafting is restricted to certain districts and seasons (winter or summer). Stranded kelps were found on all beaches. Most kelps had only one stipe (one individual), although we also frequently found coalesced holdfasts with mature males and females, which would facilitate successful rafting dispersal, gamete release, and reproduction upon arrival. High biomasses of stranded kelps occurred in the northern-central (30°S-33°S) and southernmost districts (37°S-42°S), and lower biomasses in the northernmost (28°S-30°S) and southern-central districts (33°S-37°S). The highest percentages and sizes of epibionts (Lepas spp.), indicative of prolonged floating periods, were found on stranded kelps in the northernmost and southernmost districts. Based on these results, we conclude that rafting dispersal can vary regionally, being more common in the northernmost and southernmost districts, depending on intrinsic (seaweed biology) and extrinsic factors (shore morphology and oceanography) that affect local supply of kelps and regional hydrodynamics.

Giant kelp vegetative propagation: Adventitious holdfast elements rejuvenate senescent individuals of the Macrocystis pyrifera "integrifolia" ecomorph.

Recent findings on holdfast development in the giant kelp highlighted its key importance for Macrocystis vegetative propagation. We report here for the first time the development of adventitious holdfasts from Macrocystis stipes. Swellings emerge spontaneously from different areas of the stipes, especially in senescent or creeping individuals. After being manually fastened to solid substrata, these swellings elongated into haptera, which became strongly attached after 1 month. Within 4 months, new thalli increased in size and vitality, and developed reproductive fronds. Our results suggest the usage of these structures for auxiliary attachment techniques. These could act as a backup, when primary holdfasts are weak, and thus improve the survival rate of the giant kelp in natural beds.