RESUMO
An accurate, economic, rapid, reliable spectrofluorimetric assay was developing for the assay of definite anti-depressant drugs namely Amisulpride and Milnacipran hydrochloride, in those pharmaceutical preparation and biological fluid. The suggested method was established on the detection of quenching process resulting from the action of AMS and MCN to the native fluorescent EosinY. A binary complex between selected medications and Eosin Y was established in acetate buffer (0.2â¯M) at pHâ¯3.3 & 4.0 for AMS and MCN respectively. The relative fluorescence capacity was determined at λexâ¯=â¯301.8â¯nm and λemâ¯=â¯542.7â¯nm. The calibration graphs had been linear through extent from 0.02 to 0.3 and 0.1-1⯵gâ¯mL-1, to both dugs respectively. Detection limits have been 0.0047 & 0.0188⯵gâ¯mL-1 while quantitation limits have been 0.0141 & 0.0569⯵gâ¯mL-1 to AMS & MCN respectively. Developed assay has been validated in agreement with ICH recommendations. Due to high sensitivity of the described assay, it allows the quantitation of anti-depressant drugs through biological fluid.