The action of fish peptide Orpotrin analogs on microcirculation
Journal of peptide science
; 17(3): 192-199, 23, Nov 2010.
Article
em En
| SES-SP, SESSP-IBPROD, SES-SP, SESSP-IBACERVO
| ID: biblio-1064390
Biblioteca responsável:
BR78.1
Localização: BR78.1
ABSTRACT
In order to investigate the relationshipbetween theprimary structure of Orpotrin, a vasoactive peptidepreviously isolated from the freshwater stingray Potamotrygon gr. orbignyi, and its microcirculatory effects, three Orpotrin analogs were synthesized. The analogs have a truncated N-terminal with a His residue deletion and two substituted amino acid residues, where one Nle is substituted for one internal Lys residue and the third analog has a substitution of a Pro for an Ala (Orp-desH1, Orp- Nle and Orp-Pro/Ala, respectively). Only Orp-desH1 could induce a lower vasoconstriction effect compared with the natural Orpotrin, indicating that besides the N-terminal, the positive charge of Lys and the Pro residues located at the center of the amino acid chain is crucial for this vasoconstriction effect. Importantly, the suggestions made with bioactive peptides were based on the molecular modeling and dynamics of peptides, the presence of key amino acids and shared activity in microcirculation, characterized by intravital microscopy. Moreover, this study has demonstrated that even subtle changes in the primary structure of Orpotrin alter the biological effects of this native peptide significantly, which could be of interest for biotechnological applications.
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Coleções:
06-national
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BR
Base de dados:
SES-SP
/
SESSP-IBACERVO
/
SESSP-IBPROD
Limite:
Animals
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article