Your browser doesn't support javascript.
loading
Follicular survival, activation of primordial follicles and DNA fragmentation after storage of goat ovaries at 35ºC in supplemented Minimal Essential Medium
Gonçalves, Rodrigo José de Sousa; Cavalcante, Agnes Yasmin Pitombeira; Gouveia, Bruna Bortoloni; Lins, Thae Lanne Barbosa Gama; Barberino, Ricássio de Sousa; Menezes, Vanúzia Gonçalves; Barros, Vanessa Raquel Pinto de; Santos, Luciana da Paz dos; Santos, Jamile Maiara da Silva; Figueiredo, José Ricardo de; Matos, Maria Helena Tavares de.
Afiliação
  • Gonçalves, Rodrigo José de Sousa; Universidade Federal do Vale de São Francisco. Laboratory of Cell Biology, Cytology and Histology. Nucleus of Biotechnology Applied to Ovarian Follicle Development. Petrolina. BR
  • Cavalcante, Agnes Yasmin Pitombeira; Universidade Federal do Vale de São Francisco. Laboratory of Cell Biology, Cytology and Histology. Nucleus of Biotechnology Applied to Ovarian Follicle Development. Petrolina. BR
  • Gouveia, Bruna Bortoloni; Universidade Federal do Vale de São Francisco. Laboratory of Cell Biology, Cytology and Histology. Nucleus of Biotechnology Applied to Ovarian Follicle Development. Petrolina. BR
  • Lins, Thae Lanne Barbosa Gama; Universidade Federal do Vale de São Francisco. Laboratory of Cell Biology, Cytology and Histology. Nucleus of Biotechnology Applied to Ovarian Follicle Development. Petrolina. BR
  • Barberino, Ricássio de Sousa; Universidade Federal do Vale de São Francisco. Laboratory of Cell Biology, Cytology and Histology. Nucleus of Biotechnology Applied to Ovarian Follicle Development. Petrolina. BR
  • Menezes, Vanúzia Gonçalves; Universidade Federal do Vale de São Francisco. Laboratory of Cell Biology, Cytology and Histology. Nucleus of Biotechnology Applied to Ovarian Follicle Development. Petrolina. BR
  • Barros, Vanessa Raquel Pinto de; Universidade Federal do Vale de São Francisco. Laboratory of Cell Biology, Cytology and Histology. Nucleus of Biotechnology Applied to Ovarian Follicle Development. Petrolina. BR
  • Santos, Luciana da Paz dos; Universidade Federal do Vale de São Francisco. Laboratory of Cell Biology, Cytology and Histology. Nucleus of Biotechnology Applied to Ovarian Follicle Development. Petrolina. BR
  • Santos, Jamile Maiara da Silva; Universidade Federal do Vale de São Francisco. Laboratory of Cell Biology, Cytology and Histology. Nucleus of Biotechnology Applied to Ovarian Follicle Development. Petrolina. BR
  • Figueiredo, José Ricardo de; Universidade Estadual do Ceará. Faculdade de Medicina Veterinária. Laboratory of Manipulation of Oocyte and Preantral Follicles. Fortaleza. BR
  • Matos, Maria Helena Tavares de; Universidade Federal do Vale de São Francisco. Laboratory of Cell Biology, Cytology and Histology. Nucleus of Biotechnology Applied to Ovarian Follicle Development. Petrolina. BR
Acta Vet. Brasilica ; 11(1): 50-56, mar. 2017. ilus, tab, graf
Article em En | VETINDEX | ID: biblio-1453075
Biblioteca responsável: BR68.1
Localização: BR68.1
ABSTRACT
This study evaluated the effect of caprine ovarian tissue transportation conditions (medium supplementation and transportation duration) on the morphology, DNA fragmentation and development of cultured and non-cultured preantral follicles. After the fragmentation of ovaries, one fragment was fixed (fresh control) while the remaining slices were placed individually in two different conservation media (Minimal Essential Medium - MEM without supplementation or supplemented MEM, i.e. MEM+) and stored at 35ºC for 6 or 12 h without (non-cultured) or with a subsequent 5-day in vitro culture in supplemented alfa-MEM. After transportation, followed or not by in vitro culture, the fragments were processed for histological and Terminal deoxynucleotidyl transferase (TdT) mediated dUTP nick-end labeling (TUNEL) examination. For the preserved and non-cultured fragments, the percentages of normal follicles after the storage of ovarian tissue in MEM+ for 6 h and the DNA fragmentation rates after preservation in MEM for 6 h and MEM+ for 6 or 12 h were maintained similar to the fresh control. However, all cultured treatments reduced the proportion of normal follicles and increased the percentage of TUNEL-positive cells as compared to the fresh control and non-cultured treatments. On the contrary, all culture conditions (except after preservation in MEM for 6 h) promoted an increase in primordial follicle activation. In conclusion, the use of an enriched medium (MEM+) during ovary transportation is preferable to maintain satisfactory rates of normal follicles after the preservation of caprine ovarian tissue at 35ºC for up to 6 h, without affecting the ability of the primordial follicle to grow in vitro.
Assuntos
Palavras-chave

Texto completo: 1 Base de dados: VETINDEX Limite: Animals Idioma: En Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Base de dados: VETINDEX Limite: Animals Idioma: En Ano de publicação: 2017 Tipo de documento: Article