The
association between
colorectal cancer and
human papillomavirus (HPV)
infection is still unproven. The aim of this study was to investigate the presence of high-
risk HPV (HR-HPV)
DNA in colorectal
tissues from Cuban
patients. A total of 63 colorectal
formalin-fixed
paraffin-embedded
tissues were studied (24
adenocarcinoma, 18
adenoma, and 21 colorectal
tissues classified as benign
colitis).
DNA from colorectal samples was analysed by
quantitative real-time polymerase chain reaction to detect the most clinically relevant high HR-HPV types (HPV-16, -18, -31, -33, -45, -52, and -58).
Associations between histologic findings and other
risk factors were also analysed. Overall, HPV
DNA was detected in 23.8% (15/63) of the samples studied.
Viral infections were detected in 41.7% of
adenocarcinoma (10/24) and 27.7% of
adenoma cases (5/18). HPV
DNA was not found in any of the negative cases. An
association between histological
diagnosis of
adenocarcinoma and
HPV infection was observed (odd ratio = 4.85, 95%
confidence interval = 1.40-16.80, p = 0.009). The only
genotypes identified were
HPV 16 and 33. Viral loads were higher in
adenocarcinoma, and these cases were associated with
HPV 16. This study provides molecular evidence of HR-
HPV infection in colorectal
adenocarcinoma tissues from Cuban
patients.