ABSTRACT It is widely known that
high fat diet (HFD) can contribute to the advent of
health problems. Recent studies have indicated that
obesity imposes a
hemodynamic overload to the
kidneys. In order to further investigate such
injuries, two groups of six
Swiss mice each were fed with a controlled AIN93G
diet or a high fat (AIN93G modified)
diet for eight weeks.
Blood samples were collected to determine the hormonal,
lipid profile,
glucose,
urea, and
creatinine levels. Histopathological and immunohistochemical
analysis were carried out to
analysis the
kidney damage. Fractions of renal
membranes were prepared to assess the Na,K-
ATPase activity,
lipid peroxidation, total
cholesterol, and
phospholipid content. The results indicated that the
blood lipid profile,
urea and
creatinine was not altered by the HFD. On the other
hand, it was observed in HFD
diet mice elevated
glucose blood levels along with an augment on
insulin and a decrease on
corticosterone release. HFD provoked a reduction in the diameter of the convoluted tubules and
cell volume in
Bowman's capsule and an increased number of positive
cells with Na,K-
ATPase, but reduced the Na,K-
ATPase activity and the
cholesterol content in the
kidney cell membrane but favored the
lipid peroxidation.