Evaluation of the impact of orally administered carbohydrates on postprandial blood glucose levels in different pre-clinical models
Braz. j. pharm. sci
; 52(4): 761-769, Oct.-Dec. 2016. tab
Article
em En
| LILACS
| ID: biblio-951870
Biblioteca responsável:
BR40.1
Localização: BR40.1
ABSTRACT
ABSTRACT We developed a pre-clinical model in which to evaluate the impact of orally administered carbohydrates on postprandial blood glucose levels. For this purpose, we compared the effects of different carbohydrates with well-established glycemic indexes. We orally administered (gavage) increasing amounts (0.2, 0.4, 0.6, 0.8, and 1.0 g/kg) of sucrose and lactose to rats which had been fasted for 6 h or 15 h, respectively. In part of the experiments we administered frutose (gavagem). Three different models were compared for measuring postprandial blood glucose levels a) evaluation of interstitial glucose concentrations by using a real time continuous glucose monitoring system; b) evaluation of glucose levels in blood obtained from the rat tail; c) evaluation of serum glucose levels in blood collected after decapitation. Our results showed that blood obtained from the tails of 15-h fasted rats was the best model in which to evaluate the effect of carbohydrates on postprandial blood glucose levels.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Limite:
Animals
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article