Propuesta de un panel MIRU-VNTR 5 para el tamizaje de aislamientos clínicos de Mycobacterium tuberculosis en México / Proposal of a five MIRU-VNTR panel to screen clinical isolates of Mycobacterium tuberculosis in Mexico
Enferm. infecc. microbiol. clín. (Ed. impr.)
; 33(9): 609-612, nov. 2015. tab, graf
Article
em Es
| IBECS
| ID: ibc-144637
Biblioteca responsável:
ES1.1
Localização: BNCS
RESUMEN
INTRODUCCIÓN:
La tuberculosis es un problema de salud pública que se distribuye de forma diferenciada en regiones de México. El presente trabajo propone un panel, con el mínimo número de MIRU-VNTR (mycobacterial interspersed repetitive unit-variable number tandem repeats), para la diferenciación preliminar de aislamientos clínicos de Mycobacterium tuberculosis (M. tuberculosis).MÉTODO:
Se realizó el panel completo de 24 MIRU-VNTR a 65 aislamientos clínicos de M. tuberculosis provenientes de diferentes regiones geográficas de México.RESULTADOS:
Se presenta un panel de cinco loci MIRU-VNTR para discriminar aislamientos clínicos de M. tuberculosis de tres diferentes regiones geográficas de México.CONCLUSIONES:
La utilización del panel MIRU-VNTR 5 podría utilizarse como tamizaje durante la caracterización genotípica de aislamientos clínicos de M. tuberculosis en MéxicoABSTRACT
INTRODUCTION:
Tuberculosis is a public health problem across Mexico. This paper aims to select a panel, with a minimum number of repetitive elements (MIRU-VNTR) for genotypic characterization of Mycobacterium tuberculosis (M. tuberculosis) clinical isolates.METHOD:
In this study, a full panel of 24 MIRU-VNTR loci was used to discriminate 65 clinical isolates of M. tuberculosis from three different geographical regions of Mexico. Those loci with the highest discriminatory power were subsequently selected.RESULTS:
The panel, including five loci, was obtained by selecting the highest values of allelic diversity among the genotypes obtained. The dendrogram, generated by the panel MIRU-VNTR 5, showed a high discriminatory power with 65 unique genotype profiles and formed clusters according to the geographical region of origin.CONCLUSIONS:
The panel MIRU-VNTR 5 can be useful for characterizing clinical isolates of M. tuberculosis in Mexico
Texto completo:
1
Coleções:
06-national
/
ES
Base de dados:
IBECS
Tipo de estudo:
Prognostic_studies
/
Screening_studies
Limite:
Humans
País/Região como assunto:
Mexico
Idioma:
Es
Ano de publicação:
2015
Tipo de documento:
Article