Replication of Rocio virus in primary cultures of mouse neural cells
Souto, Adriano da Paixão; Casseb, Samir Mansour Moraes; Melo, Karla Fabiane Lopes de; Martins Filho, Arnaldo Jorge; Franco, Edna Cristina Santos.
SOUTO, Adriano da Paixão et al. Replication of Rocio virus in primary cultures of mouse neural cells. Einstein São Paulo, v. 21, n. eAO0160, 2023. DOI: https://doi.org/10.31744/einstein_journal/2023AO0160. Disponível em: https://www.scielo.br/j/eins/a/9RMpdqCBtCj6myN4LHVTQqb/?format=pdf&lang=en.
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em Inglês
| IED | ID: ied-6840
This study verified the replication efficiency of the Rocio virus in a primary culture of mouse neural cells.
Methods:
Mixed primary cultures (neurons/glia) obtained from the brains of newborn isogenic BALB/c mice were inoculated with Rocio virus on the 7th day of culture, and the development of cytopathogenic effects was monitored. The infection was confirmed via immunocytochemistry (anti-ROCV), while viral replication was quantified in infected primary cultures. The titration method used depended on the infection period.Results:
Rocio virus efficiently infected primary cultured neural cells, with the highest viral titer causing cytopathic changes was observed at 2 days post infection. The virus-infected primary culture survived for up to 7 days post infection, and viral load quantitation showed viral replication kinetics compatible with the cell death kinetics of cultures.Conclusion:
The findings of this study suggest that mouse neural cell primary cultures support Rocio virus replication and could be used as an alternative system for studying Flavivirus infection in the central nervous system. / Instituto Evandro Chagas/ Ministry of Health and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)/Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
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