Shear stress modulates tumour cell adhesion to the endothelium.
Biorheology
; 40(1-3): 41-5, 2003.
Article
em En
| MEDLINE
| ID: mdl-12454385
ABSTRACT
The adhesion of breast adenocarcinoma cells (MDA-MB-231) to human umbilical vein endothelial cells (HUVEC) was studied in whole blood and under varying flow conditions. This study was done on HUVEC either kept under static conditions or pre-conditioned in flow for 2 hours at a shear stress of 5 or 13 dyn/cm(2). Coverslips coated by HUVEC were placed in a parallel plate perfusion chamber and perfused at a shear rate of 300 or 1500 sec(-1) with heparin-anticoagulated blood containing 111In labelled MDA-MB-231 cells. We report here the optimal conditions for studying the adhesion of MDA-MB-231 to endothelial cells under shear constraints corresponding to those observed into small and medium sized arteries.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Limite:
Female
/
Humans
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article