Using a kinase screen to investigate the constituents of the sponge Stelletta clavosa obtained from diverse habitats.
Bioorg Med Chem
; 12(21): 5631-7, 2004 Nov 01.
Article
em En
| MEDLINE
| ID: mdl-15465341
ABSTRACT
Fourteen collections of the marine sponge Stelletta clavosa have been obtained from diverse Indo-Pacific locations in order to conduct a comparison of their major constituents. The dichloromethane extract of one collection (no. 00369) exhibited activity in a c-Raf-1 kinase assay. Bioactivity-directed isolation resulted in the known porphyrin analogs pyropheophorbide a (2) and purpurin 18 methyl ester (3). Further spectroscopic screening of the various sponge extracts resulted in the isolation of four swinholide polyketides, a carotenoid, and three diketopiperazines. Pyropheophorbide a (2) exhibited the best IC(50) among the porphyrin type compounds (IC(50)<0.31microg/mL). This prompted further screening of 2 against a panel of 85 kinases.
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01-internacional
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MEDLINE
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Animals
Idioma:
En
Ano de publicação:
2004
Tipo de documento:
Article