[Application of Ac/Ds transposon system to genetate marker gene free transgenic plants in rice].
Sheng Wu Gong Cheng Xue Bao
; 19(6): 668-73, 2003 Nov.
Article
em Zh
| MEDLINE
| ID: mdl-15971577
ABSTRACT
It is critical to generate marker gene free transgenic plants for retransformating or eliminating the potential harmfulness of marker gene and its product. In this study, Ac/Ds transposon system was developed for removal of hpt selection marker gene to obtain marker-free transgenic plants in rice ( Oryza sativa L.). Ds element containing the interesting gene bar was constructed next to the selection marker gene hpt to get Ds-T-DNA. Rice plants were transformed by Agrobacterium tumefaciens EHA105 containing Ac-T-DNA and Ds-T-DNA respectively. Rice plant containing single copy Ac-T-DNA was crossed with plant containing single copy Ds-T-DNA to obtain the F1 plant containing both Ac and Ds elements. F1 plant was self-crossed to produce F2 progeny in which T-DNA insert and transposed Ds element segregated independently. Two plants contained Ds element but no hpt marker gene in total 100 F2 plants. The result indicated that Ac/Ds transposon system could be used as a vector system for generating marker gene free transgenic plants in rice.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
Zh
Ano de publicação:
2003
Tipo de documento:
Article