Enzyme-electropolymer-based amperometric biosensors: an innovative platform for time-temperature integrators.
J Agric Food Chem
; 53(23): 8866-73, 2005 Nov 16.
Article
em En
| MEDLINE
| ID: mdl-16277377
ABSTRACT
A novel exogenous time-temperature integrator (TTI) based on an amperometric glucose oxidase biosensor is presented. The TTI consists of the enzyme entrapped within an electrochemically generated poly(o-phenylenediamine) (PoPD) thin film deposited on the interior wall of a platinum (Pt) or a platinized stainless steel (Pt-SS) capsule. After thermal treatment, the TTI is mounted in a continuous flow system and connected to a potentiostat for amperometric detection of residual enzyme activity. A measurement is completed within 10 min. Isothermal treatments were carried out between 70 and 79.7 degrees C. Thermal inactivation of the immobilized enzyme followed apparent first-order kinetics with z values of 6.2 +/- 0.6 and 6.6 +/- 0.8 degrees C for Pt and Pt-SS capsules, respectively. These z values suggest that the proposed TTIs have the potential to assess pasteurization processes that target microorganism such as Listeria monocytogenes and Escherichia coli O157H7.
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01-internacional
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MEDLINE
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article