Your browser doesn't support javascript.
loading
T-lymphocyte reconstitution following rigorously T-cell-depleted versus unmodified autologous stem cell transplants.
Te Boekhorst, P A W; Lamers, C H J; Schipperus, M R; Hintzen, R Q; van der Holt, B; Cornelissen, J J; Löwenberg, B; Gratama, J W.
Afiliação
  • Te Boekhorst PA; Department of Hematology, Erasmus Medical Center, Rotterdam, The Netherlands. p.teboekhorst@erasmusmc.nl
Bone Marrow Transplant ; 37(8): 763-72, 2006 Apr.
Article em En | MEDLINE | ID: mdl-16518423
We compared the kinetics of T-cell recovery after extensive ex vivo and in vivo T-cell depleted autologous stem cell transplantation (SCT) for multiple sclerosis (MS; n=8) with unmodified SCT for hematological malignancies (HM; n=39). Both patient group showed a very protracted recovery of 'naive' CD4(+), 45R0(-) ( approximately CD45RA(+)) T-cells. Within the 'primed' CD4(+), 45R0(+) T-cells, the 'central memory' cells expressing the CD62L and CD27 markers were the slowest to recover. The repopulating T-cells were highly activated, as shown by increased expression of HLA-DR and the apoptosis marker CD95. The capability of CD4(+) and CD8(+) T-cells to produce IFN-gamma, IL-2 and TNF-alpha had reached normal ranges from 2 months post SCT onwards. Unexpectedly, the kinetics of T-cell recovery between 3 and 12 months post transplant was similar in T-depleted and unmodified SCT. Before SCT, the HM patients showed lymphopenia of all T-cell subsets, upregulated HLA-DR and CD95 expression and increased cytokine responses. We suggest that the similar kinetics of T-cell recovery in the two patient groups may be explained by the susceptibility to apoptosis of the activated CD4(+) T-cells in the autografts of the HM patients. This susceptibility to apoptosis would interfere with a swift and sustained CD4(+) T-cell regeneration post SCT.
Assuntos
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Limite: Adult / Female / Humans / Male / Middle aged Idioma: En Ano de publicação: 2006 Tipo de documento: Article
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Limite: Adult / Female / Humans / Male / Middle aged Idioma: En Ano de publicação: 2006 Tipo de documento: Article