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[Antibodies against hepatitis C virus. Experience with a second generation test]. / Anticuerpos contra el virus de la hepatitis C. Experiencias con una prueba de segunda generación.
Garassini, M A; Ortega, F; Alvarado, M; Valdés, A; Díaz, F; Dugarte, I; Serrano, L.
Afiliação
  • Garassini MA; Departamento de Gastroenterología, Inmunología y Nefrología, Hospital Militar Dr. Carlos Arvelo, Caracas.
G E N ; 45(3): 183-9, 1991.
Article em Es | MEDLINE | ID: mdl-1726913
A second generation recombinant immunoblot assay to detect antibodies against Hepatitis C virus (RIBA II, Ortho Diagnostic Systems) was applied to 30 serum samples repeatedly reactive to ELISA anti-HCV c100-3, 11 from hemodialysis patients, 11 from patients with chronic hepatitis and 8 from patients with cirrhosis. The assay detects individual antibodies directed to 4 antigenic components of the C virus, 2 antigens, c100 and 5-1-1 contained in the original ELISA assay and 2 new antigens, c33c and c22-3 the latter structural in nature. From the total of 30 samples, 23 (77%) were reactive, 4 (13%) indeterminate and 3 (10%) non reactive to the RIBA II assay. Much variation was observed regarding the response to each individual antigen. c22-3 was the most frequently reactive with the highest intensity. RIBA II assay confirmed a high percentage of the reactive results with the ELISA c-100-3, serum samples that were non reactive to RIBA showed a low optical density with ELISA. c22-3 was the most sensitive antigenic component.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: Es Ano de publicação: 1991 Tipo de documento: Article
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: Es Ano de publicação: 1991 Tipo de documento: Article