[Identification and clinical evaluation of lung cancer serum biomarker L-lactate dehydrogenase B].
Zhonghua Jie He He Hu Xi Za Zhi
; 30(8): 577-81, 2007 Aug.
Article
em Zh
| MEDLINE
| ID: mdl-17988549
ABSTRACT
OBJECTIVE:
To identify potential biomarkers related with lung cancer metastasis.METHODS:
Conditional media proteins collected from a primary non-small cell lung cancer cell line (NSCLC) NCI-H226 and its brain metastatic subline H226Br were analyzed by SDS-PAGE and MALDI-TOF-MS. Twelve biomarkers were identified, of which LDHB was significantly up-regulated in H226Br cell and was further validated using ELISA in sera including 105 lung cancer samples, 41 pneumonia and pulmonary tuberculosis samples and 65 healthy samples.RESULTS:
The levels of LDHB were specifically elevated in NSCLC sera [A value 0.485 (0 - 1.415)] compared with pneumonia and pulmonary tuberculosis [A value 0.187 (0 - 0.609), P < 0.01] and healthy group [A value, 0.159 (0 - 0.524), P < 0.01] and were progressively increased with the clinical stage. At the cutoff point 0.260 (A value) on the ROC curve, the sensitivity, specificity and total accuracy of LDHB were 81%, 70% and 76% respectively.CONCLUSION:
These findings demonstrated that secretome could open up a possibility to identify novel biomarkers related with cancer occurrence and metastasis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Adult
/
Aged
/
Female
/
Humans
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Male
/
Middle aged
Idioma:
Zh
Ano de publicação:
2007
Tipo de documento:
Article