[Aberrant expression of sperm protein 17 enhances migration of ovarian cancer cell line HO-8910].
Zhonghua Nan Ke Xue
; 14(11): 982-6, 2008 Nov.
Article
em Zh
| MEDLINE
| ID: mdl-19102497
ABSTRACT
OBJECTIVE:
To explore the effects of aberrant expression of sperm protein 17 (Sp17) on the migration of the ovarian cancer cell line HO-8910.METHODS:
The recombinant plasmid pEGFP-Sp17 containing Sp17 and enhanced green fluorescent protein gene was transfected into the human ovarian cancer cell line HO-8910 with Lipofectamine 2000. The expression of Sp17 was examined by RT-PCR and Western blot, and the cell migratory capability detected by Transwell chamber assays.RESULTS:
Sp17 was expressed as a fusion protein with EGFP after transfected. There was a significant difference in the migratory cell number of the transfected and the control cells (156.6 +/- 14.9/HP vs 39.3 +/- 8.53/HP, P < 0.05).CONCLUSION:
The aberrant expression of Sp17 greatly enhances the migration of ovarian cancer cells.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Limite:
Female
/
Humans
/
Male
Idioma:
Zh
Ano de publicação:
2008
Tipo de documento:
Article