Analysis of autophagy during infections of Cryptococcus neoformans.

ABSTRACT

Cryptococcus neoformans is a yeastlike fungus that causes a lethal meningoencephalitis in a broad spectrum of immunocompromised patients and has become the most common cause of meningitis due to AIDS-related infections in Africa. Key to the development of new agents to control and prevent this infection is the identification of cellular mechanisms required for pathogenesis. Survival of the fungus within the hostile and nutrient-deprived environments of the host has recently been shown to depend on the induction of autophagy, whereby the cell recycles nutrients by slowly digesting itself in a regulated fashion. Further study of the role of autophagy during infection by C. neoformans requires the use of markers of autophagy that are specially adapted to the fungus within the mammalian host.