[Chiral separation of fluvastatin enantiomers with in vitro cellular method].
Zhejiang Da Xue Xue Bao Yi Xue Ban
; 40(1): 12-6, 2011 01.
Article
em Zh
| MEDLINE
| ID: mdl-21319367
ABSTRACT
OBJECTIVE:
To establish a chiral separation method for determination of fluvastatin enantiomer with in vitro cellular model.METHODS:
The determination was performed on Chiralpak AD column (4.6 mm × 250 mm); and the phase consisted of hexane-isopropanol-trifluoroacetic acid (90100.1) at a flow rate of 0.5 ml/min with UV detection of 239 nm.RESULT:
The standard curve was linear over the concentration range of 20 µmol/L-300 µmol/L (r² = 0.9993, r² = 0.9997). The recovery for this assay was (99.4 ± 0.8)%, precision for inter-assay and intra-assay was <10 %.CONCLUSION:
The normal-phase HPLC chiral separation method was accurate and suitable for study on the stereoselectivity of fluvastatin with in vitro cellular model.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
Zh
Ano de publicação:
2011
Tipo de documento:
Article