[Construction methods of tissue engineered cell sheet].

OBJECTIVE: To summarize the recent progress of construction methods of engineered cell sheet and to forecast the possible prospect. METHODS: The recent original articles about investigation and application of engineered cell sheet were reviewed. Several common methods were selected and expounded. RESULTS: The construction methods of engineered cell sheet mainly include temperature-responsive culture dish, salmon atelocollagen, magnetic force, surface roughness, and polyelectrolytes, which may overcome the limits of traditional tissue engineering methods. CONCLUSION: The construction methods of engineered cell sheet are feasible and have a bright future in the clinical application.