Your browser doesn't support javascript.
loading
Methods for growing and titrating African swine fever virus: field and laboratory samples.
Carrascosa, Angel L; Bustos, M Jose; de Leon, Patricia.
Afiliação
  • Carrascosa AL; Centro de Biologia Molecular "Severo Ochoa" (CSIC-UAM), Universidad Autonoma de Madrid, Madrid, Spain.
  • Bustos MJ; Centro de Biologia Molecular "Severo Ochoa" (CSIC-UAM), Universidad Autonoma de Madrid, Madrid, Spain.
  • de Leon P; Centro de Biologia Molecular "Severo Ochoa" (CSIC-UAM), Universidad Autonoma de Madrid, Madrid, Spain.
Curr Protoc Cell Biol ; Chapter 26: 26.14.1-26.14.25, 2011 Dec.
Article em En | MEDLINE | ID: mdl-22161547
Growing African swine fever virus (ASFV) isolates obtained mainly from the field, but also engineered in the laboratory, is a critical step for diagnosis, titration, or virus infection studies. This unit describes a set of methods and protocols to produce and titrate any ASFV strain in cell cultures. The procedures include (1) basic techniques to prepare virus-sensitive target cells; (2) strategies for growth, concentration, and purification of virus stocks; and (3) the semi-quantitative (end dilution) and quantitative (plaque) assays for the determination of viral titers, and the use of different ASFV-sensitive cells as targets for virus production and titration.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Guideline Limite: Animals Idioma: En Ano de publicação: 2011 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Guideline Limite: Animals Idioma: En Ano de publicação: 2011 Tipo de documento: Article