Construction of an amperometric D-amino acid biosensor based on D-amino acid oxidase/carboxylated mutliwalled carbon nanotube/copper nanoparticles/polyalinine modified gold electrode.
Anal Biochem
; 437(1): 1-9, 2013 Jun 01.
Article
em En
| MEDLINE
| ID: mdl-23399389
ABSTRACT
An improved D-amino acid biosensor was constructed based on covalent immobilization of D-amino acid oxidase onto carboxylated mutliwalled carbon nanotube/copper nanoparticles/polyalinine hybrid film electrodeposited on gold electrode. The biosensor exhibited an optimal response within 2s at pH 8.0 and 30°C when polarized at 0.09 V. There was a linear relationship between biosensor response (µA) and D-alanine concentration ranging from 0.001 to 0.7 mM. The sensitivity of the biosensor was 54.85 µA cm(-2) mM(-1) with a lower limit of detection of 0.2 µM (signal/noise=3). The enzyme electrode was used 150 times over a period of 4 months. The biosensor measured the d-amino acid level in fruit juices.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Limite:
Animals
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article