Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease.
Genetics
; 194(4): 1029-35, 2013 Aug.
Article
em En
| MEDLINE
| ID: mdl-23709638
ABSTRACT
We have adapted a bacterial CRISPR RNA/Cas9 system to precisely engineer the Drosophila genome and report that Cas9-mediated genomic modifications are efficiently transmitted through the germline. This RNA-guided Cas9 system can be rapidly programmed to generate targeted alleles for probing gene function in Drosophila.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Limite:
Animals
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article