A simple and high yield purification of Crotalus adamanteus phospholipases A2.
Biochim Biophys Acta
; 380(3): 501-5, 1975 Mar 24.
Article
em En
| MEDLINE
| ID: mdl-237535
ABSTRACT
A new, high yield, procedure for the purification of the phospholipases A2 of Crotalus adamanteus venom is described. The procedure involves precipitation of the bulk of venom proteins with 50% isopropanol, precipitation of the enzymes from the isopranol soluble material with neodynium chloride, and final purification on DEAE cellulose. An overall yield of approximately 80% is achieved.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Ano de publicação:
1975
Tipo de documento:
Article